基于小RNA测序技术和RT-PCR检测云南省大樱桃病毒
Identification of viruses infecting sweet cherry in Yunnan Province by small RNA sequencing and RT-PCR
查看参考文献26篇
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文摘
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我国是大樱桃的主要产区,目前种植面积位居世界首位,随着种植面积的不断扩大,病毒病严重制约了大樱桃生产。本研究对云南3个大樱桃主要繁育基地(陆良、宣威和丽江)的6个大樱桃品种的病毒病发生情况调查,发现主要症状为植株矮化、小叶、叶片皱缩、畸形、斑驳、叶片褐色斑点等症状,发病率在10% ~ 30%之间。对每个品种混合样品进行小RNA测序,并利用RT-PCR对小RNA测序分析得到的病毒种类进行验证。结果表明,在陆良样品中检测到樱桃病毒A(cherry virus A,CVA)、李属坏死环斑病毒(prunus necrotic ringspot virus,PNRSV)、李树皮坏死与茎痘伴随病毒(plum bark necrosis stem pitting-associated virus,PBNSPaV)、柑橘叶斑病毒(citrus leaf blotch virus,CLBV)和樱桃小果病毒-1(little cherry virus-1,LChV-1);宣威样品中检测到CVA和PNRSV;丽江样品中检测到CVA、PNRSV、PBNSPaV、CLBV和樱桃小果病毒-2(little cherry virus-2,LChV-2)。序列分析表明,这5种病毒分别与GenBank中登录注册的病毒核苷酸序列均具有较高的一致性。其中,PNRSV在不同种植区的品种中检出率较高,推测有可能是目前云南大樱桃种植区的主要病毒种类,其危害情况有待于进一步研究。 |
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其他语种文摘
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China is now the world's biggest sweet cherry producing country by growth area.severe viral diseases curbed cherry production in Yunnan province,China.A survey on the occurrence of cherry viral diseases in Lijiang,Luliang and Xuanwei counties of Yunnan province showed that viral diseases occurred in 3 cultivation areas on 6 varieties,with infection rates of 10%-30%.The major symptoms included dwarfing,malformation,leaf narrowing,leaf shrinking,and mottled,mosaic and brown spots on leaves.Mixed samples from 3 areas were collected for each variety,and the presence of viruses was tested by small RNA deep sequencing combined with RT-PCR.The results showed that 5 viruses were detected from Luliang.They were Cherry virus A (CVA),prunus necrotic ningspot virus (PNRSV),little cherry virus-1 (LChV-1),plum bark necrosis and pitting-associated virus (PBNSPaV) and citrus leaf blotch virus (CLBV).There were also CVA and PNRSV detected in Xuanwei.CVA,PNRSV,PBNSPaV,CLBV and LChV-2 were detected in Lijiang.Sequence analyses showed that the nucleotide sequences of these virus displayed high homology to related viruses in GenBank.PNRSV showed a higher relevance ratio than other viruses.Thus,we speculate that it is a dominant virus in cherry-producing areas of Yunnan. |
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来源
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植物病理学报
,2023,53(5):950-958 【核心库】
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DOI
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10.13926/j.cnki.apps.001311
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关键词
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云南
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大樱桃
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小RNA深度测序
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病毒种类
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地址
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1.
云南省农业科学院高山经济植物研究所, 丽江, 674199
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云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室;;农业部西南农业基因资源与种质创制重点实验室, 昆明, 650223
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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云南实验室建设专项
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云南省基础研究专项重大项目
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云南省农业科学院云南省农业生物技术重点实验室基金
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文献收藏号
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CSCD:7616500
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