多花黄精炭疽病菌的鉴定、生物学特性及防治药剂筛选
Identification,biological characterization,and fungicide screening of the pathogen causing Polygonatum cyrtonema Hua anthracnose
查看参考文献65篇
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文摘
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炭疽菌属(Colletotrichum spp.)引起的多花黄精炭疽病,给多花黄精生产造成了较大经济损失。本文从重庆市万州区多花黄精种植基地采集具有典型炭疽病症状的多花黄精病叶,采用组织分离法分离、单孢分离法纯化病原菌。依据致病性测定证明其均有致病性,结合病原菌形态学特征及其ITS、ACT、CHS、TUB和MAT序列的多基因联合分析,确定其病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides)、果生炭疽菌(C.fructicola)和冬麦炭疽菌(C.liriopes),其中胶孢炭疽菌(C.gloeosporioides)为优势种,冬麦炭疽菌(C.liriopes)侵染黄精引起炭疽病是首次报道。通过平板培养和产孢量统计,测定冬麦炭疽菌(C.liriopes)在不同培养基、温度、碳源、氮源、pH值和光照条件下的生物学特性;采用菌丝生长速率法测定3种炭疽病菌对6种杀菌剂的敏感性。冬麦炭疽菌(C.liriopes)最佳生长温度为25~ 28 ℃,最适生长pH为6.0 ~ 7.0,全光照利于菌丝生长;PDA最利于病原菌菌丝生长和产孢;葡萄糖利于菌丝生长和产孢,甘油利于菌丝生长但不利于产孢;最佳氮源是酵母。参试的6种药剂对3种炭疽病菌菌丝生长均有抑制效果,其中苯醚甲环唑10% WP和嘧鲜胺45% ME对3种炭疽病菌菌丝生长的抑制作用较强,可作为防治多花黄精炭疽病的优选药剂。本研究为多花黄精炭疽病的识别与防治提供了科学依据。 |
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其他语种文摘
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Anthracnose caused by Colletotrichum spp.on Polygonatum cyrtonema Hua has occurred heavily,which are responsible for important economic loss on the production of P.cyrtonema.In this study,the sample with typical anthracnose symptoms on P.cyrtonema leaves were collected from P.cyrtonema plantations in Chongqing,China.Isolation made from the diseased tissues were inoculated into PDA plates and three fungal strains were purified by single spore isolation.The pathogenic assays on the detected leaves of P.cyrtonema plants were conducted which had fulfilled the Koch's postulates.Combined with the morphological characteristics and molecular analysis of the ITS,ACT,CHS,TUB,and MAT sequences,the pathogens were identified as Colletotrichum gloeosporioides,C.fructicola and C.liriopes in which C.gloeosporioides was the dominant species,and this is the first report of C.liriopes caused anthracnose on P.cyrtonema plants in China mainland.To study C.liriopes's biological characteristics,mycelial growth and sporulation quantity of C.liriopes were measured under different medium,temperatures,carbon and nitrogen sources,pH values,and light conditions on plate cultures.The sensitivities of the tested strains to 6 fungicides were determined based on mycelial growth inhibition method.The results of characteristics for C.liriopes showed that optimum growth at 28~30 ℃,pH 6.0~7.0,and full illumination;PDA was optimum to mycelium growth and spore production;glucose was beneficial to mycelium growth and spore production,while glycerol was good for mycelium growth but bad for spore production;the suitable nitrogen source for mycelium growth and spore production was yeast extracts.All C.gloeosporioides,C.fructicola and C.liriopes were sensitive to 6 tested fungicides in which Difenoconazole 10% WP and Prochloraz 45% ME had better inhibition effects against the three species.The results have provided scientific evidence and reference for screening fungicides to control Colletotrichum spp.anthracnose on P.cyrtonema. |
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来源
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植物病理学报
,2023,53(5):796-809 【核心库】
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DOI
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10.13926/j.cnki.apps.001020
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关键词
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多花黄精
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炭疽菌
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病原鉴定
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冬麦炭疽菌
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药剂敏感性
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地址
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1.
重庆三峡学院生物与食品工程学院, 重庆, 404120
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三峡库区道地药材绿色种植与深加工重庆市工程实验室, 三峡库区道地药材绿色种植与深加工重庆市工程实验室, 重庆, 404120
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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重庆市技术创新与应用发展专项重点项目
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重庆市教委重点项目
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文献收藏号
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CSCD:7616486
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