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佛手叶部褐斑病病原菌鉴定及防治药剂筛选
Identification of the pathogen causing leaf brown spot on Citrus medica var.sarcodactylis and screening of fungicides for the control of the disease

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窦涛 1,2   范安阳 1,2   崔广林 1,2   李隆云 1,2 *   宋旭红 1,2 *  
文摘 为明确引起佛手叶部褐斑病的病原,本研究从重庆市垫江区采集病叶,采用组织分离法分离并纯化得到佛手叶部褐斑病菌菌株CQACMM 22001,依据科赫氏法则验证其致病性。根据病原菌形态学特征及分子生物学鉴定结果,将该菌株鉴定为棉链格孢(Alternaria gossypina)。生物学特性研究表明,该菌对光照不敏感,生长最适培养基为燕麦琼脂培养基,最适生长温度为30 ℃,最适pH为7.0,最适碳源为可溶性淀粉和乳糖,最适氮源为氯化铵。杀菌剂毒力测定结果表明,咪鲜胺、苯醚甲环唑、异菌脲和啶酰菌胺对该菌有较强的抑制作用,EC_(50)分别为0.008、2.024、4.700和6.294 mg·L~(-1) 。本研究为佛手叶部褐斑病的有效防控提供了科学依据。
其他语种文摘 In order to identify the pathogen causing leaf brown spot on Citrus medica var.sarcodactylis,leaves with typical symptoms of brown spot were collected from Dianjiang District of Chongqing.The fungal strain CQACMM 22001 was isolated by tissue separation and then was purified,and its pathogenicity was verified according to Koch's postulates.Based on morphological characteristics and the result of molecular identification,the strain was identified as Alternaria gossypina.Research on biological characteristics of the pathogen demonstrated that it is insensitive to light;OA medium is the most suitable medium for its mycelial growth;the optimal temperature for the growth of the pathogen is 30 ℃,the optimal pH is 7.0,the optimal carbon sources are soluble starch and lactose,and the optimal nitrogen source is ammonium chloride.Toxicity test results showed that prochloraz,difenoconazole,iprodione,and boscalid had stronger inhibitory effect on A.gossypina,with EC_(50) valuesof 0.008,2.024,4.700,and 6.294 mg·L~(-1),respectively.This study provides scientific basis for the prevention and control of leaf brown spot of C.medica var.sarcodactylis.
来源 植物病理学报 ,2023,53(5):769-778 【核心库】
DOI 10.13926/j.cnki.apps.001606
关键词 佛手 ; 叶片褐斑病 ; 棉链格孢 ; 生物学特性 ; 毒力测定
地址

1. 重庆市中药研究院, 重庆, 400065  

2. 中国中医科学院中药资源中心重庆分中心, 重庆, 400065

语种 中文
文献类型 研究性论文
ISSN 0412-0914
学科 植物保护
基金 国家中药材产业技术体系 ;  重庆市科研机构激励引导专项 ;  重庆市现代山地特色高效农业技术体系
文献收藏号 CSCD:7616483

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1 李润根 龙牙百合褐斑病病原的鉴定、生物学特性及室内药剂筛选 核农学报,2024,38(8):1487-1495
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