金银花黄萎病的病原鉴定及生防菌剂防控
Pathogen identification and biological agent control of honeysuckle Verticillium wilt
查看参考文献47篇
|
文摘
|
2020年河北省金银花主产区发生了一种金银花新病害,本文针对此病害开展了病原菌分离、鉴定及生物防治研究。采用常规组织分离法进行病原菌分离,通过盆栽试验完成分离物的致病性测定,通过形态学观察和分子生物学方法对分离物进行鉴定,并确定金银花病原菌的生理型、生理小种和交配型。同时在田间条件下评价微生物杀菌剂"10亿芽胞/克枯草芽胞杆菌可湿性粉剂"对金银花新病害的防治效果。从金银花病株维管束中分离获得7个真菌纯培养物,柯赫氏法则证明这7株真菌均为金银花新病害的病原菌。显微观察发现这7株真菌的分生孢子梗均具轮状分枝,在培养基上均可形成微菌核,据此初步确定其为轮枝菌属真菌(Verticillium) 。rDNA-ITS序列比对和大丽轮枝菌特异性引物扩增证明这7株真菌均为大丽轮枝菌(V.dahliae) 。进一步采用相关特异性引物扩增证明,这7株大丽轮枝菌均为非落叶型菌株、2号生理小种、MAT1-2-1交配型。致病性测定结果表明,金银花黄萎菌还能够侵染棉花、茄子、马铃薯、向日葵等作物,在4种作物上均表现出较强的致病性。田间采用微生物杀菌剂"10亿芽胞/克枯草芽孢杆菌可湿性粉剂"穴施可有效减轻金银花黄萎病的发生,防治效果达86.36%,增产11.7%。在我国首次报道由大丽轮枝菌引起的金银花黄萎病,"10亿芽胞/克枯草芽孢杆菌可湿性粉剂"可用于该病害的有效防控。 |
|
其他语种文摘
|
A new disease of honeysuckle (Lonicera japonica) occurred in Julucounty of Hebei province in recent years.In order to effectively control the disease,the isolation,identification of the pathogen were carried out,and biological control of the new disease was conducted.The conventional tissue isolate method was used to isolate pathogen.Pathogenicities of the isolates were determined by pot testing in the greenhouse.The isolates were identified by morphological observation and molecular methods.And physiological type,physiological race and mating type of the isolates were identified by molecular methods.The effect of 1×10~9 spores·g~(-1) Bacillus subtilis WP against the new disease of honeysuckle was evaluated under field condition.Seven fungal isolates were obtained from the diseased honeysuckle plants,and these isolates were all confirmed as the pathogen of L.japonica by Koch's Rule testing.All isolates showed wheel branched conidiophores and could produce black radial microsclerotia on the medium.These characters were identical to the fungi of Verticillium genus.By internal transcribed spacer (ITS) sequence alignment and PCR amplification with V.dahliae specific primers,these isolates were identified as V.dahliae.By PCR amplification with specific primers,all the 7 isolates were further identified as nondefoliated strains,physiological race 2 and MAT1-2-1 mating type.Pathogenicity tests showed that V.dahliae from honeysuckle could infect cotton,eggplant,potato,sunflower and showed strong pathogenicity.Field experiment demonstrated that this microbial fungicide could significantly decrease honeysuckle Verticillium wilt and increase honeysuckle yields.The control effect was 86.36% and the yield increased 11.7%.To our knowledge,it is the first report of Verticillium wilt on L.japonica occurred in China.The 1×10~9 spores·g~(-1) B.subtilis WP was an excellent microbial preparation for honeysuckle Verticillium wilt control. |
|
来源
|
植物病理学报
,2023,53(4):642-654 【核心库】
|
|
DOI
|
10.13926/j.cnki.apps.000849
|
|
关键词
|
金银花
;
病原鉴定
;
大丽轮枝菌
;
生理型
;
生理小种
;
交配型
;
致病性
;
生物防治
|
|
地址
|
河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术中心, 农业农村部华北北部作物有害生物综合治理重点实验室, 保定, 071000
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0412-0914 |
|
学科
|
植物保护 |
|
基金
|
河北省农林科学院创新工程项目
;
河北省第四批高端人才发展规划项目
|
|
文献收藏号
|
CSCD:7616465
|
参考文献 共
47
共3页
|
|
1.
Gun S F.
Studies on occurrence and control technique of the major pests on honeysuckle in Han zhong area (in Chinese),2006
|
CSCD被引
1
次
|
|
|
|
|
2.
Yi S R. The control technology of pest and diseases to Lonicera japonica (in Chinese).
亚太传统医药,2011,7(7):23-25
|
CSCD被引
3
次
|
|
|
|
|
3.
Yao Y H. Diseases and pests of honeysuckle flower (Lonicera Japonica) and its integrated control (in Chinese).
凯里学院学报,2008,26(3):56-59
|
CSCD被引
9
次
|
|
|
|
|
4.
Jian M L. 广东省金银花主要病虫害调查初报.
广东农业科学,2011(14):74-75
|
CSCD被引
9
次
|
|
|
|
|
5.
Wu C F. Present situation and counter measures to control pests and diseases of Lonicera japonica in Henan (in Chinese).
湖北农业科学,2012,51(13):2732-2735
|
CSCD被引
2
次
|
|
|
|
|
6.
Hu Z C. Investigation on the pests and diseases of Lonicera macrathoides Hand-Maxx in Longhui county (in Chinese).
湖南林业科技,2005,32(1):47-48
|
CSCD被引
1
次
|
|
|
|
|
7.
Li Z M. Present situation and counter measures to control Lonicera japonica Thunb. pest and disease in Hunan (in Chinese).
中国农村小康科技,2010(12):66-68
|
CSCD被引
4
次
|
|
|
|
|
8.
Chen Y C. Preliminary study on green prevention and control techniques of honeysuckle disease and insect pests in Suiyang (in Chinese).
南方农业,2020,14(11):31-32,34
|
CSCD被引
1
次
|
|
|
|
|
9.
Yang Y L. Molecular identification of dieback pathogenic fungi in Lonicera macranthoides(in Chinese).
西南农业学报,2015,28(5):2017-2111
|
CSCD被引
1
次
|
|
|
|
|
10.
Cen Y H. Occurrence characteristics and control methods of Lonicera japonica Thunb. Seedling blight (in Chinese).
北方园艺,2013(8):135-137
|
CSCD被引
1
次
|
|
|
|
|
11.
Bellemain E. ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases.
BMC Microbiology,2010,10(1):1-9
|
CSCD被引
46
次
|
|
|
|
|
12.
Mercado-Blanco J. Simultaneous detection of the defoliating and nondefoliating Verticillium dahliae pathotypes in infected olive plants by duplex, nested polymerasechain reaction.
Plant Disease,2003,87(12):1487-1494
|
CSCD被引
11
次
|
|
|
|
|
13.
Mercado-Blanco J. Detection of the defoliating pathotype of Verticillium dahliae in infected olive plants by nested PCR.
European Journal of Plant Pathology,2002,108:1-13
|
CSCD被引
10
次
|
|
|
|
|
14.
Mercado-Blanco J. Detection of the nonde foliating pathotype of Verticillium dahliae in infected olive plants by nested PCR.
Plant Pathology,2001,50:609-619
|
CSCD被引
14
次
|
|
|
|
|
15.
Ronnie D J. Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing.
Proceedings of the National Academy of Sciences of the United States of America,2012,109(13):5110-5115
|
CSCD被引
1
次
|
|
|
|
|
16.
Short D P. Verticillium dahliae race 2-specific PCR reveals a high frequency of race 2 strains in commercial spinach seed lots and delineates race structure.
Phytopathology,2014,104(7):779-785
|
CSCD被引
6
次
|
|
|
|
|
17.
Usami T. A sexual fungus Verticillium dahliae is potentially heterothallic.
Journal of General Plant Pathology,2009,75(6):422-427
|
CSCD被引
7
次
|
|
|
|
|
18.
Fang Z D.
Research method for plant pathology (2nd Ed) (in Chinese),1998:63
|
CSCD被引
1
次
|
|
|
|
|
19.
Lu X Y. 一种丹参新病害的病原菌鉴定及致病力测定.
植物病理学报,2021,51(2):163-170
|
CSCD被引
4
次
|
|
|
|
|
20.
Fang H L. Research progress in germplasm resources and breeding of Lonicera japonica(in Chinese).
江西农业学报,2017,29(9):45-50
|
CSCD被引
2
次
|
|
|
|
|
了解气象卫星更多信息，请访问