陕西省华中五味子根腐病病原鉴定与防治药剂筛选
Pathogen identification and effective fungicides screening of root rot of Schisandra sphenanthera in Shaanxi Province
查看参考文献21篇
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文摘
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陕西省柞水、石泉、镇安县等地的华中五味子种植业,已成为当地乡村振兴的支柱产业。随着人工种植面积的不断扩大,其根部腐烂病十分突出,严重影响华中五味子产业发展。为明确引起华中五味子根腐病的病原,本研究从陕西省商洛市的柞水县、镇安县和宝鸡市的陇县五味子种植基地采集病株标本,经组织分离法和致病性检验获得华中五味子根腐病病原菌,并根据形态学以及ITS-TEF-1α-β-Tubulin序列串联分析,将其鉴定为层出镰刀菌(Fusarium proliferatum),杀菌剂室内抑菌活性测定结果表明,供试11种杀菌剂中吡唑醚菌酯、叶菌唑、咯菌腈和戊唑醇对其抑菌作用最强,EC_(50)值分别为0.021 0、0.083 3、0.380 7和0.403 5 μg·mL~(-1) 。本研究为陕西地区华中五味子根腐病的有效防控提供重要指导依据。 |
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其他语种文摘
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Schisandra chinensis planting has become a pillar industry for the local rural revitalization for Zhashui,Shiquan and Zhen'an counties in Shaanxi Province.With the continuous increasing of artificial planting areas,root rot has become a main problem which seriously affects the development of S.chinensis industry in Shaanxi Province.In order to identify the pathogen of S.chinensis root rot in Shaanxi Province,the specimens of diseased plants were collected from S.chinensis planting bases in Zhashui County and Zhen' an County in Shangluo City and Long County in Baoji City in Shaanxi Province.The pathogen of S.chinensis root rot was obtained by tissue isolation and pathogenicity testing.Pathogenicity test and phylogenetic analysis based on ITS-TEF-1α-β-Tubulin sequences showed that Fusarium proliferatum was the pathogen.The laboratory antifungal activity screening test showed that the selected 11 fungicides had certain inhibitory effects on the pathogen,among which Pyraclostro-bin,Metconazole,Saphire and Tebuconazole exhibited relative higher inhibitory effects,with EC_(50) of 0.021 0,0.083 3,0.380 7 and 0.403 5 μg·mL~(-1) respectively.This study provides an important guiding basis for the effective prevention and control of S.chinensis root rot in Shaanxi Province. |
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来源
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植物病理学报
,2023,53(3):491-497 【核心库】
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DOI
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10.13926/j.cnki.apps.000834
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关键词
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华中五味子
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根腐病
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分子鉴定
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药剂筛选
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地址
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西北农林科技大学, 杨凌, 712100
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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国家重点研发计划
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陕西林业科技创新重点专项
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文献收藏号
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CSCD:7616444
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