生防链霉菌YC2-3的筛选、鉴定及其对小麦茎基腐病的防治效果
Screening,identification and detection of biocontrol effect of strain YC2-3 on wheat crown rot
查看参考文献35篇
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文摘
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由假禾谷镰刀菌(Fusarium pseudograminearum,Fp)引起的小麦茎基腐病是小麦生产上一种根茎部的真菌病害,严重影响小麦的产量和品质。为了开发防治小麦茎基腐病的链霉菌生防资源,本研究采用稀释涂布平板法和皿内对峙法从健康植株根际土壤中分离、筛选高效拮抗假禾谷镰刀菌的链霉菌。结果表明,10份供试土样中共分离到69株链霉菌,其中菌株YC2-3对假禾谷镰刀菌的拮抗作用最强,抑菌带宽度大于14 mm。基于系统发育分析、形态特征、培养特征和生理生化特性对YC2-3进行多相分类鉴定,将菌株YC2-3鉴定为直丝紫链霉菌(Streptomyces rectiviolaceus)。抑菌谱测试结果表明YC2-3具有广谱的抗真菌活性。温室盆栽试验结果表明,菌株YC2-3处理的治疗组和预防组均可以降低小麦茎基腐病的发病率和病情指数,生防效果分别为69.01%和73.94%。菌株YC2-3可以产生多种真菌细胞壁降解酶、铁载体和ACC脱氨酶,具有溶解有机磷的能力,还具有多种抗生素合成的核心基因。实时定量PCR(RT-qPCR)试验结果显示,YC2-3灌根处理12 h后,小麦茎基部组织中过氧化物酶基因TaPOD被激活表达。以上结果表明S.rectiviolaceus YC2-3具有开发成为小麦茎基腐病生防制剂的潜力。 |
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其他语种文摘
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Crown rot of wheat,caused by the pathogenic fungal Fusarium pseudograminearum (Fp),is a devastating disease of wheat,which dramatically affects wheat production and its quality.To screen effective candidate biocontrol agents against Fp,streptomycetes were isolated from rhizosphere soil samples using dilution plating method and plate confrontation method.In this study,69 streptomycetes were obtained from 10 soil samples.Among them,strain YC2-3 demonstrated the strongest inhibitory activity against Fp,producing a radius of inhibition zone >14 mm.Strain YC2-3 was identified as Streptomyces rectiviolaceus based on phylogenetic analyses,morphological characteristics,cultural characteristics,and biochemical characteristics.Strain YC2-3 exhibited a broad-spectrum antifungal activity.Under greenhouse conditions,the strain YC2-3 inoculated at 24 h before (protective effect) and 24 h after (curative effect) Fp inoculation,significantly reduced the incidence rate and disease index of wheat crown rot,and the biocontrol efficacy were 69.01% and 73.94%,respectively.Bioassays revealed that strain YC2-3 can produce various extracellular hydrolytic enzymes,siderophore,ACC deaminase,and has the ability to solubilize organic phosphate.Expression profiling of defense-related marker genes showed that the peroxidases gene TaPOD was activated at 12 h after the wheat was treated with YC2-3.These results suggest that S.rectiviolaceus YC2-3 has the potential to be used as a biological agent for the control of wheat crown rot. |
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来源
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植物病理学报
,2023,53(3):473-484 【核心库】
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DOI
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10.13926/j.cnki.apps.000805
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关键词
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生物防治
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小麦茎基腐病
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假禾谷镰刀菌
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直丝紫链霉菌
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地址
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西北农林科技大学植物保护学院, 杨凌, 712100
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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陕西省重点研发计划
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文献收藏号
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CSCD:7616442
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