西瓜噬酸菌效应蛋白AopK的功能研究
Functional study of effector protein AopK of Acidovorax citrulli
查看参考文献45篇
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文摘
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瓜类细菌性果斑病是西甜瓜产业中最重要病害之一,其病原菌西瓜噬酸菌的Ⅲ型分泌系统效应蛋白在致病过程中起重要作用。XopK为黄单胞菌效应蛋白,具有E3泛素连接酶活性,其在西瓜噬酸菌中的同源基因aopK鲜有研究。为鉴定效应蛋白AopK并探究其功能,本研究构建了西瓜噬酸菌AAC00-1菌株的aopK缺失突变株和互补菌株,发现基因aopK的缺失显著降低病原菌在西瓜幼苗上的致病力。通过AopK在本氏烟中的亚细胞定位实验,显示AopK定位于植物的细胞质膜。转录水平、外泌性及BAX共表达试验,证明aopK的表达受HrpX和HrpG正调控,其产物能够通过Ⅲ型分泌系统分泌到细胞外,并且能够抑制烟草叶片细胞中活性氧产生、胼胝质沉积以及细胞坏死产生,具有潜在抑制植物防卫反应的毒性功能。以上结果表明AopK为西瓜噬酸菌的Ⅲ型效应蛋白,研究结果为进一步探究其与寄主植物间的互作机理奠定了基础。 |
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其他语种文摘
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The type Ⅲsecreted effectors (T3Es) play important roles in the pathogenesis of Acidovorax citrulli,the causal agent of bacterial fruit blotch in watermelon and melon plants.XopK is a T3E with E3 ubiquitin ligase activity in Xanthomonas spp.The homolog of XopK,designated as AopK,in A.citrulli has not been investigated.To explore the function of AopK,the aopK deletion mutant and complementary strain were constructed in A.citrulli wild-type strain AAC00-1.The pathogenicity assay showed that the deletion of the gene aopK significantly reduces the virulence of A.citrulli on watermelon seedlings.Subcellular localization analysis of AopK was performed using Nicotiana benthamiana.Results revealed that AopK was delivered to the cytoplasmic membrane of N.benthamiana.The transcriptional expression level,secretory and BAX co-expression analyses showed that the expression of aopK was positively regulated by HrpX and HrpG.AopK was secreted into extracellular matrix through the Type Ⅲ secretion system.AopK can inhibit the production of reactive oxygen species,callose deposition and cell necrosis in N.benthamiana,and affects the virulence via inhibiting plant defense responses.In summary,our data indicated that AopK is a T3E in A.citrulli.Our data laid a foundation for further exploring the mechanism of interaction between A.citrulli and host plants. |
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来源
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植物病理学报
,2023,53(3):436-446 【核心库】
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DOI
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10.13926/j.cnki.apps.000644
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关键词
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西瓜噬酸菌
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效应蛋白
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AopK
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功能研究
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地址
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1.
吉林农业大学植物保护学院, 长春, 130118
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中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京, 100193
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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国家西甜瓜产业技术体系
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国家重点研发计划
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国家自然科学基金国家杰出青年科学基金
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北京青年基金
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中央级公益性科研院所基本科研业务专项基金
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中国农业科学院创新工程
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文献收藏号
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CSCD:7616438
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