番茄褐色皱果病毒胶体金免疫试纸条的研制
Development of colloidal gold immunochromatographic strip for detection of tomato brown rugose fruit virus
查看参考文献26篇
|
文摘
|
番茄褐色皱果病毒(tomato brown rugose fruit virus, ToBRFV)是一种新发病毒,严重威胁番茄的安全生产。为了快速、简便地检测该病毒,我们制备了ToBRFV胶体金免疫试纸条。本研究以ToBRFV粒子为免疫原,通过杂交瘤技术制备了17个抗ToBRFV的单克隆抗体。将不同单抗两两组合分别作为胶体金标记抗体和硝酸纤维素膜检测线上的捕获抗体,共获得272个配对组合的胶体金试纸条。通过特异性测定筛选到一组配对抗体制备的试纸条能够在5 min内特异识别ToBRFV,而与番茄斑驳花叶病毒、番茄花叶病毒、烟草花叶病毒、黄瓜花叶病毒、辣椒轻斑驳病毒、马铃薯X病毒、马铃薯Y病毒、番茄褪绿病毒、番茄斑萎病毒、番茄黄化曲叶病毒等无交叉反应。灵敏度分析表明,该试纸条可从稀释12 800倍的番茄叶片病汁液中检测到ToBRFV,也可检测到50 ng ToBRFV粒子。本研究制备的胶体金试纸条使用方便,灵敏度高,特异性强,适合田间大批量样品检测,可用于ToBRFV的精准监测及早期预警。 |
|
其他语种文摘
|
Tomato brown rugose fruit virus(ToBRFV)is an emerging virus and seriously threatens the safety of tomato production. To achieve rapid and simple detection of the virus, we developed a colloidal gold immunochromatographic strip for ToBRFV. Here, we used ToBRFV particles as the immunogen and produced 17 monoclonal antibody lines by hybridoma technology. Two different monoclonal antibodies were individually used for the colloidal gold-labeled antibody and the capture antibody on the detection line of the nitrocellulose membrane and combined to prepare colloidal gold immunochromatographic strips with 272 paired combinations. The immunochromatographic strip prepared with one paired antibodies could specifically recognize ToBRFV within 5 minutes, without reacting with tomato mottle mosaic virus, tomato mosaic virus, tobacco mosaic virus, cucumber mosaic virus, pepper mild mottle virus, potato virus X, potato virus Y,PVY, tomato chlorosis virus, tomato spotted wilt virus, or tomato yellow leaf curl virus. The sensitivity test results showed that the minimum detection threshold was 12 800 times for ToBRFV-infected tomato leaf extracts and 50 ng ToBRFV particles. The resultant colloidal gold immunochromatographic strip, which is sensitive and specific for ToBRFV, easy to use, and competent for large-scale sample detection, can be applied in the precise monitoring and early warning of ToBRFV. |
|
来源
|
植物病理学报
,2022,52(6):976-983 【核心库】
|
|
DOI
|
10.13926/j.cnki.apps.000606
|
|
关键词
|
胶体金免疫试纸条
;
检测
;
特异性
;
番茄褐色皱果病毒
|
|
地址
|
1.
山东农业大学植物保护学院, 山东省农业微生物重点实验室, 泰安, 271018
2.
山东省农业技术推广中心, 济南, 250014
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0412-0914 |
|
学科
|
植物保护 |
|
基金
|
国家自然科学基金
;
泰山学者建设工程
|
|
文献收藏号
|
CSCD:7415492
|
参考文献 共
26
共2页
|
|
1.
Yan Z Y. Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection.
Journal of Integrative Agriculture,2021,20(7):1871-1879
|
CSCD被引
4
次
|
|
|
|
|
2.
Luria N. A new Israeli Tobamovirus isolate infects tomato plants harboring Tm-22 resistance genes.
PLoS One,2017,12(1):e0170429
|
CSCD被引
11
次
|
|
|
|
|
3.
Salem N. A new tobamovirus infecting tomato crops in Jordan.
Archives of Virology,2016,161(2):503-506
|
CSCD被引
10
次
|
|
|
|
|
4.
van de Vossenberg B T L H. Real-time tracking of tomato brown rugose fruit virus (ToBRFV) outbreaks in the Netherlands using Nextstrain.
PLoS One,2020,15(10):e0234671
|
CSCD被引
3
次
|
|
|
|
|
5.
Beris D. First report of tomato brown rugose fruit virus infecting tomato in Greece.
Plant Disease,2020,104(7):2035
|
CSCD被引
3
次
|
|
|
|
|
6.
Amer M A. First report of tomato brown rugose fruit virus on tomato in Egypt.
New Disease Reports,2020,41:24
|
CSCD被引
5
次
|
|
|
|
|
7.
Alfaro-Fernandez A. First report of tomato brown rugose fruit virus in tomato in Spain.
Plant Disease,2021,105(2):515
|
CSCD被引
1
次
|
|
|
|
|
8.
Skelton A. First report of tomato brown rugose fruit virus in tomato in the United Kingdom.
New Disease Reports,2019,40:12
|
CSCD被引
4
次
|
|
|
|
|
9.
Fidan H. First report of tomato brown rugose fruit virus on tomato in Turkey.
New Disease Reports,2019,39:18
|
CSCD被引
5
次
|
|
|
|
|
10.
Panno S. First report of tomato brown rugose fruit virus on tomato crops in Italy.
Plant Disease,2019,103(6):1443
|
CSCD被引
5
次
|
|
|
|
|
11.
Ling K S. First report of tomato brown rugose fruit virus infecting greenhouse tomato in the United States.
Plant Disease,2019,103(6):1439
|
CSCD被引
7
次
|
|
|
|
|
12.
Camacho-Beltran E. Occurrence of tomato brown rugose fruit virus infecting tomato crops in Mexico.
Plant Disease,2019,103(6):1440
|
CSCD被引
3
次
|
|
|
|
|
13.
Yan Z Y. First report of tomato brown rugose fruit virus infecting tomato in China.
Plant Disease,2019,103(11):2973
|
CSCD被引
8
次
|
|
|
|
|
14.
Menzel W. First report of tomato brown rugose fruit virus infecting tomato in Germany.
New Disease Reports,2019,39:1
|
CSCD被引
8
次
|
|
|
|
|
15.
Alkowni R. Molecular identification of tomato brown rugose fruit virus in tomato in Palestine.
Journal of Plant Pathology,2019,101(3):719-723
|
CSCD被引
7
次
|
|
|
|
|
16.
Gao W Z. Detection and homology analysis of tomato brown rugose fruit virus in some areas of Beijing, Shandong and Shaanxi provinces(in Chinese).
中国蔬菜,2021(4):48-52
|
CSCD被引
1
次
|
|
|
|
|
17.
Ma Z Y. Molecular characterization and pathogenicity of an infectious cDNA clone of tomato brown rugose fruit virus.
Phytopathology Research,2021,3(1):14
|
CSCD被引
3
次
|
|
|
|
|
18.
Davino S. Tomato brown rugose fruit virus: seed transmission rate and efficacy of different seed disinfection treatments.
Plants,2020,9(11):1615
|
CSCD被引
4
次
|
|
|
|
|
19.
Levitzky N. The bumblebee Bombus terrestris carries a primary inoculum of Tomato brown rugose fruit virus contributing to disease spread in tomatoes.
PLoS One,2019,14(1):e0210871
|
CSCD被引
7
次
|
|
|
|
|
20.
Chanda B. Comparative analysis of host range, ability to infect tomato cultivars with Tm-22 gene and real-time RT-PCR detection of tomato brown rugose fruit virus.
Plant Disease,2021,105(11):3643-3652
|
CSCD被引
3
次
|
|
|
|
|
了解气象卫星更多信息，请访问