两株木霉共培养发酵提高对小麦苗期茎基腐病的防治效果
Co-culturation of two Trichoderma strains enhanced control efficiency against wheat crown rot at seedling stage
查看参考文献35篇
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文摘
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通过平板对峙培养法、孢子萌发法和温室盆栽法筛选高效防治小麦苗期茎基腐病的木霉共培养菌株。从17株供试菌株中,筛选出3株平板抑制率较高的木霉菌株:深绿木霉HB20111、哈茨木霉LTR-2和TW21990,并进行两两组合共培养发酵。孢子萌发试验表明,HB20111+TW21990共培养发酵滤液对假禾谷镰孢孢子萌发抑制率最高,达71.83%,较单菌株HB20111和TW21990分别提高了6.13%和19.53%(P<0.05)。温室条件下,HB20111+TW21990共培养发酵液500×稀释浸种效果最好,对小麦苗期茎基腐病防治效果和幼苗质量增长率分别达78.81%和130.80%,较单菌株HB20111和TW21990的分别提高了17.88%、30.46%和50.05%、61.22%(P<0.05);荧光定量检测结果表明,与单菌株相比,HB20111+TW21990共培养发酵显著降低假禾谷镰孢在小麦幼苗根围土、根际土、根系和茎基部中的拷贝数(P<0.05),且假禾谷镰孢拷贝数与病情指数呈正相关,与幼苗鲜质量呈负相关。本研究发现HB20111+TW21990共培养发酵具有增效作用,对小麦苗期茎基腐病的防治效果显著高于单菌株。 |
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其他语种文摘
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We evaluated the inhibitory efficiency of 17 Trichoderma strains against Fusarium pseudograminearum(Fp), the predominant causal agent of wheat crown rot through dual plate confrontation assay. Among them, T. atroviride HB20111, T. harzianum LTR-2 and TW21990 exhibited higher inhibition rates, which were selected for co-culturing. The fermentation filtrate of HB20111 +TW21990 had 71.83% inhibition rate for Fp spore germination, which significantly increased by 6.13% and 19.53% respectively, compared with single strain HB20111 and TW21990. In greenhouse condition, the 500×dilution fermentation broth was the most effective treatment to control wheat seedling crown rot and increase the weight of seedling fresh at rate of 78.81% and 130.80%. Compared with the single strain HB20111 and TW21990, the efficiencies of the paired were significantly increased by 17.88%, 30.46% and 50.05%, 61.22%, respectively. As expected, real-time quantitative PCR(qPCR) analysis showed that co-culturing fermentation treatment could significantly reduce the Fp DNA copy numbers in the bulk soil, rhizosphere soil, root and basal stem, which were positively correlated with the disease index while negatively with the seedlings fresh weight. Therefore, the co-culturation of HB20111 + TW21990 had a synergistic effect on control of wheat crown rot to a greater extent than the single strain. |
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来源
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植物病理学报
,2022,52(3):425-433 【核心库】
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DOI
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10.13926/j.cnki.apps.000759
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关键词
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深绿木霉HB20111
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哈茨木霉TW21990
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共培养发酵
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小麦茎基腐病
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防治效果
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地址
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1.
齐鲁工业大学(山东省科学院)生态研究所, 济南, 250103
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齐鲁工业大学(山东省科学院)生物工程学院, 济南, 250353
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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山东省重点研发计划项目(重大科技创新类竞争择优类第一批)
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山东省重点研发计划(重大科技创新工程)
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山东省自然基金博士基金
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济南市泉城高端项目
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山东省科学院院地产学研协同创新基金
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山东省科学院科教产融合创新试点工程(国际合作)项目
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文献收藏号
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CSCD:7233910
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