小西葫芦绿斑驳花叶病毒生物学特性及传播方式
Biological characteristics and transmission modes of zucchini green mottle mosaic virus
查看参考文献34篇
文摘
|
小西葫芦绿斑驳花叶病毒(Zucchini green mottle mosaic virus, ZGMMV)是我国新报道的侵染葫芦科作物的烟草花叶病毒属病毒。从感染病毒的葫芦叶片中分离出ZGMMV,摩擦接种西瓜(Citrullus lanatus)、葫芦(Lagenaria siceraria)、西葫芦(Cucurbita pepo)、南瓜(Cucurbita moschata)、丝瓜(Luffa cylindrica)、黄瓜(Cucumis sativus)、甜瓜(Cucumis melo)、西方烟(Nicotiana occidentalis)、本生烟(Nicotiana benthamiana)和心叶烟(Nicotiana glutinosa),通过症状观察、血清学和分子生物学检测明确症状特点和寄主范围;研究病毒的种子传播,以及整枝打杈和滴灌对于病毒的传播。结果表明:ZGMMV可侵染所有供试植物;叶片症状主要为花叶、斑驳和褪绿斑,西瓜茎蔓出现坏死斑、果实倒瓤,甜瓜果实表皮出现褪绿斑; ZGMMV可通过整枝打杈等农事操作机械传播,也可通过滴灌的水在土壤中传播。该病毒在西瓜、葫芦和甜瓜上可以种传,种传率均约为1%。研究结果有助于制订该病害的综合防控策略。 |
其他语种文摘
|
Zucchini green mottle mosaic virus(ZGMMV), belonging to the genus Tobamovirus, was newly reported from China in 2018. In this study, we purified ZGMMV from field-grown bottle gourd sample, and inoculated bottle gourd(Lagenaria siceraria), watermelon(Citrullus lanatus), muskmelon(Cucumis melo), cucumber(C. sativus), zucchini(Cucurbita pepo), pumpkin(Cucurbita moschata), luffa(Luffa cylindrica), Nicotiana benthamiana, N. glutinosa and N. occidentalis at the seedling stage. Through the symptom observation, serological and molecular detection, the host symptoms and range were investigated. The results showed that ZGMMV could infect all tested plants. The symptoms were mosaic, mottle and chlorotic spots on the leaves of all inoculated plants. In addition, necrotic spots on stem and flesh decay were observed in watermelon, and chlorotic spots appeared on the fruit surface of muskmelon. We studied the transmission modes through pruning, drip irrigation and seeds. Results showed that ZGMMV can be transmitted by mechanical pruning, drip irrigation water and seeds. It was confirmed that one percent of seed transmission occurred in bottle gourd, watermelon and muskmelon. These results will help us to design effective prevention and control strategy for the virus. |
来源
|
植物病理学报
,2022,52(1):68-76 【核心库】
|
DOI
|
10.13926/j.cnki.apps.000570
|
关键词
|
小西葫芦绿斑驳花叶病毒
;
摩擦接种
;
甜瓜
;
西瓜
;
传播
;
倒瓤
|
地址
|
中国农业科学院郑州果树研究所, 郑州, 450009
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
0412-0914 |
学科
|
植物保护 |
基金
|
国家西甜瓜产业技术体系
;
中央级公益性科研院所基本科研业务专项基金
|
文献收藏号
|
CSCD:7233870
|
参考文献 共
34
共2页
|
1.
Christenhusz M J M. The number of known plants species in the world and its annual increase.
Phytotaxa,2016,261(3):201-217
|
CSCD被引
87
次
|
|
|
|
2.
Dombrovsky A. Cucumber green mottle mosaic virus: rapidly increasing global distribution, etiology, epidemiology, and management.
Annual Review of Phytopathology,2017,55(1):231-256
|
CSCD被引
9
次
|
|
|
|
3.
Antignus Y. Biological and molecular characterization of a new cucurbit-infecting tobamovirus.
Phytopathology,2001,91(6):565-571
|
CSCD被引
7
次
|
|
|
|
4.
Ainsworth G C. Mosaic disease of cucumber.
Annals of Applied Biology,1935,22(1):55-67
|
CSCD被引
20
次
|
|
|
|
5.
Orita H. Molecular and serological characterization of cucumber mottle virus, a new cucurbit-infecting tobamo-like virus.
Plant Disease,2007,91(12):1574-1578
|
CSCD被引
1
次
|
|
|
|
6.
Tan S H. The genome structure of kyuri green mottle mosaic tobamovirus and its comparison with that of cucumber green mottle mosaic tobamovirus.
Archives of Virology,2000,145(6):1067-1079
|
CSCD被引
10
次
|
|
|
|
7.
Ryu K H. Zucchini green mottle mosaic virus is a new tobamovirus; comparison of its coat protein gene with that of kyuri green mottle mosaic virus.
Archives of Virology,2000,145(11):2325-2333
|
CSCD被引
1
次
|
|
|
|
8.
Cheng Y H. Identification and characterisation of watermelon green mottle mosaic virus as a new cucurbit-infecting tobamovirus.
Annals of Applied Biology,2018,174:31-39
|
CSCD被引
1
次
|
|
|
|
9.
Li X D. Identification of tobacco mosaic virus infecting watermelon (in Chinese).
山东农业大学学报,1996,27(2):181-184
|
CSCD被引
1
次
|
|
|
|
10.
Al-Dosary N S. A novel zucchini green mottle mosaic virus (ZGMMVSA) in cucurbita pepo from Saudi Arabia.
Fifth Saudi Science Conference,2012:1-2
|
CSCD被引
1
次
|
|
|
|
11.
Li Z. First report of natural infection of zucchini green mottle mosaic virus on bottle gourd in Guangxi, China.
Plant Disease,2018,102(11):2384
|
CSCD被引
2
次
|
|
|
|
12.
Yoon J Y. Genome structure and production of biologically active in vitro transcripts of cucurbit-infecting zucchini green mottle mosaic virus.
Phytopathology,2002,92(2):156-163
|
CSCD被引
2
次
|
|
|
|
13.
Li J X. Research progress on the transmission of cucumber green mottle mosaic virus (in Chinese).
中国蔬菜,2015(1):19-24
|
CSCD被引
1
次
|
|
|
|
14.
Wu H J. The rate of seed contamination and transmission of cucumber green mottle mosaic virus in watermelon and melon (in Chinese).
中国农业科学,2011,44(7):1527-1532
|
CSCD被引
2
次
|
|
|
|
15.
Broadbent L. The epidemiology of tomato mosaic: IV. Persistence of virus on clothing and glasshouse structures.
Annals of Applied Biology,1963,52(2):233-241
|
CSCD被引
2
次
|
|
|
|
16.
Reingold V. Epide-miological study of Cucumber green mottle mosaic virus in greenhouses enables reduction of disease damage in cucurbit production.
Annals of Applied Biology,2016,168(1):29-40
|
CSCD被引
1
次
|
|
|
|
17.
Dombrovsky A. Seed transmission of toba-moviruses: aspects of global disease distribution.
Advances in Seed Biology,2017,12(6):233-260
|
CSCD被引
1
次
|
|
|
|
18.
Li J X.
Transmission modes of cucumber green mottle mosaic virus and selection of resistance inducers (in Chinese),2015
|
CSCD被引
1
次
|
|
|
|
19.
Liu H W. Pollen and seed transmission of Cucumber green mottle mosaic virus in cucumber.
Plant Pathology,2014,63(1):72-77
|
CSCD被引
7
次
|
|
|
|
20.
Choi G S. Occurrence of twotobamovirus diseases in cucurbits and control measures in Korea.
Plant Pathology Journal,2001,17:243-248
|
CSCD被引
14
次
|
|
|
|
|