淡水磷酸盐氧同位素前处理方法的优化
Optimization approach of pretreatment methods for phosphate oxygen isotopic analysis in freshwater
查看参考文献30篇
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文摘
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磷是地表水体中的关键性营养盐,在水生生态系统的物质循环与能量流动方面发挥着重要作用,研究水体中磷的来源、转化与归趋对于了解水环境的演变过程与科学保护具有重要意义.近年来,磷酸盐氧同位素(δ~(18)O_P)技术已逐渐应用于淡水环境中磷的来源示踪与生物地球化学循环研究,其样品前处理主要沿用海水方法体系.相比而言,淡水样品中PO_4~(3-)浓度通常较低,有机质和干扰离子含量却较高,复杂的样品前处理过程极大地制约了δ~(18)O_P分析在淡水环境体系的广泛应用.为此,本研究针对现有海水样品δ~(18)O_P前处理方法在地表淡水环境的适用性加以检验,并进行了三点优化改进: ①将MAGIC沉淀步骤使用的MgCl_2替换为Mg(NO_3)_2,避免了Cl-的干扰,减少AgCl杂质的生成;②调节生成Ag_3PO_4溶液pH值为8.0,保证Ag_3PO_4沉淀快速完全;③对Ag_3PO_4沉淀过程采用避光处理,降低了AgNO_3及Ag_3 PO_4可能的光解影响,提高了Ag_3PO_4的纯度,使δ~(18)O_P的测试结果更为准确.本改进方法为后续利用δ~(18)O_P技术深入探究淡水环境中磷的生物地球化学循环与生态环境效应提供了有益的方法借鉴. |
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其他语种文摘
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Phosphorus(P)is one of the key nutrients in surface water,and plays a vital role in the material cycle and energy flow in aquatic ecosystems.Hence,it is of great significance to explore the source,transformation,and fate in water body for understanding its evolution process and scientific protection of water environment.Recently,phosphate oxygen isotope technology(δ~(18)O_P)has been gradually applied in tracing the P source and studying the biogeochemical cycle in freshwater environment,which most used the sample pretreatment of seawater methods.By contrast,the concentrations of PO_4~(3-) in freshwater samples are usually low,whereas the contents of organic matter and interference ions are high.The pretreatment complexity restricted the extensive application of δ~(18)O_P analysis in freshwater ecosystems.This study examined the applicability of the existing pretreatment methods of seawater sample in surface freshwater environment,and further carried out three optimization improvements: ①We replaced MgCl_2 solution with Mg(NO_3)_2 solution in the MAGIC precipitation step to avoid the interference of Cl-and the impurities of AgCl.②The solution pH value of Ag_3PO_4 precipitation was adjusted to 8.0 to ensure the rapid and complete precipitation.③Possible photolysis effects of AgNO_3 and Ag_3 PO_4 were inhibited by using light avoidance.This modification improved the purity of Ag_3PO_4,and attained more accurate δ~(18)O_P results.The present method gives a valuable reference to further study of the biogeochemical cycle and eco-effects of P in freshwater environment using δ~(18)O_P analysis. |
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来源
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湖泊科学
,2021,33(1):319-326 【核心库】
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DOI
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10.18307/2021.0127
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关键词
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淡水环境
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磷
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磷酸盐氧同位素
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前处理方法
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改进
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地址
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1.
中国科学院地球化学研究所, 贵阳, 550081
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中国科学院大学, 北京, 100049
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1003-5427 |
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学科
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畜牧、动物医学、狩猎、蚕、蜂 |
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基金
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国家自然科学基金项目
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国家自然科学基金委员会-贵州喀斯特科学研究中心项目
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文献收藏号
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CSCD:6892512
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