不同品种和植期甘蔗白叶病自然发病率调查
Investigation on natural incidence of sugarcane white leaf in different varieties and crop cycles
查看参考文献23篇
文摘
|
甘蔗白叶病(sugarcane white leaf,SCWL)是由植原体引起的甘蔗毁灭性病害,对甘蔗生产危害极大。为明确不同品种不同植期SCWL的发病规律,2018年对我国SCWL发病最为严重的耿马芒翁和贺派蔗区进行了SCWL田间发病情况调查和巢式PCR检测分析。田间调查结果表明,不同品种田间自然发病率不同,其中粤糖60号平均发病率最高,为73.50%,柳城05-136平均发病率最低,为13.67%;不同植期田间自然发病率也有差异,新植蔗田间发病率最低,为32.38%,3年宿根蔗的田间发病率最高,为64.33%。病原检测结果表明,所有品种的阳性检出率均在90%以上,其中盈育91-59阳性检出率最低,为90.95%,柳城05-136阳性检出率最高,为96.67%,无白叶症状样品的阳性检出率为81.53%,2年和3年宿根的阳性检出率最高,均为96.67%。本研究结果表明SCWL发病率随宿根年限增加而升高,依据白叶症状进行的田间病害调查不能准确反映SCWL的真实发生情况。 |
其他语种文摘
|
Sugarcane white leaf(SCWL)is a devastating disease caused by phytoplasmas,which is extremely harmful to sugarcane production.In order to clarify the occurrence regularity of SCWL in different cultivars and different crop cycles,in 2018,we conducted field investigation and nested PCR detection of SCWL in caneplanting areas of Mangweng and Hepai in Gengma,where SCWL was the most serious in China.The results of field investigation showed that the natural incidence rate of different varieties was different.The average disease incidence rate in Yuetang60 was the highest(73.50%)while Liucheng05-136 had the lowest(13.67%).The natural incidence rate in newly planted sugarcane was the lowest(32.38%),whereas,in the three-year ratoon sugarcane it was the highest(64.33%).The results of pathogen detection showed that the positive detection rate in all varieties was above 90%.The positive detection rate of Yingyu91-59 was the lowest(90.95%)and Liucheng05-136 was the highest(96.67%),the positive detection rate of asymptomatic samples was 81.53%,and the two and three-year ratoon sugarcane had the highest positive detection rate(96.67%).The results of this study showed that the incidence of SCWL increased with the increase of crop cycle,and the field investigation based on white leaf symptoms could not accurately reflect the actual occurrence of SCWL. |
来源
|
植物病理学报
,2020,50(3):345-350 【核心库】
|
DOI
|
10.13926/j.cnki.apps.000430
|
关键词
|
植原体
;
甘蔗白叶病
;
自然发病率
;
耿马
;
植期
|
地址
|
云南省农业科学院甘蔗研究所, 云南省甘蔗遗传改良重点实验室, 开远, 661699
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
0412-0914 |
学科
|
植物保护 |
基金
|
国家自然科学基金项目
;
国家现代农业产业技术体系(糖料)建设专项资金
;
云岭产业技术领军人才培养项目
;
云南省农业基础研究联合专项
;
云南省现代农业产业技术体系建设专项资金
|
文献收藏号
|
CSCD:6747225
|
参考文献 共
23
共2页
|
1.
Zhang R Y. Group 16SrXI phytoplasma strains,including subgroup 16SrXI-B and a new subgroup,16SrXI-D,are associated with sugarcane white leaf.
International Journal of Systematic and Evolutionary Microbiology,2016,66(1):487-491
|
CSCD被引
2
次
|
|
|
|
2.
Huang Y K.
Color illustration of diagnosis and control for modern sugarcane diseases,pests,and weeds,2018
|
CSCD被引
3
次
|
|
|
|
3.
Hanboonsong Y. Transovarial transmission of sugarcane white leaf phytoplasma in the insect vector Matsumuratettix hiroglyphicus (Matsumura).
Insect Molecular Biology,2002,11(1):97-103
|
CSCD被引
5
次
|
|
|
|
4.
Hanboonsong Y. Transmission of sugarcane white leaf phytoplasma by Yamatotettix flavovittatus, a new leafhopper vector.
Journal Economic Entomology,2006,99(5):1531-1537
|
CSCD被引
6
次
|
|
|
|
5.
Rao G P. A century progress of research on phytoplasma diseases in India.
Phytopathogenic Mollicutes,2017,7(1):1-38
|
CSCD被引
3
次
|
|
|
|
6.
Tiwari A K. New efficient natural leafhopper vectors of sugarcane grassy shoot phytoplasma in India.
Sugar Tech,2017,19(2):191-197
|
CSCD被引
2
次
|
|
|
|
7.
Marcone C. Phytoplasma diseases of sugarcane.
Sugar Tech,2002,4(3/4):79-85
|
CSCD被引
6
次
|
|
|
|
8.
Wongkaew P. Differentiation of phytoplasmas associated with sugarcane and gramineous weed white leaf disease and sugarcane grassy shoot disease by RFLP and sequencing.
Theoretical and Applied Genetics,1997,95(4):660-663
|
CSCD被引
4
次
|
|
|
|
9.
Kumarasinghe N C. Identification of white leaf disease of sugarcane in Sri Lanka.
Sugar Tech,2001,3(1):55-58
|
CSCD被引
5
次
|
|
|
|
10.
Thein M M. Dispersal of leafhoppers Matsumuratettix hiroglyphicus and Yamatotettix flavovittatus (Homoptera: cicadellidae), vectors of sugarcane white leaf disease.
Applied Entomology and Zoology,2012,47(3):255-262
|
CSCD被引
5
次
|
|
|
|
11.
Wang X Y. Identification of sugarcane white leaf phytoplasma in fields and quaratine sugarcane samples in Yunnan province, China.
Sugar Tech,2015,17(1):85-88
|
CSCD被引
4
次
|
|
|
|
12.
Li W F. First report of sugarcane white leaf phytoplasma in Yunnan province, China.
Canadian Journal of Plant Pathology,2013,35(3):407-410
|
CSCD被引
6
次
|
|
|
|
13.
Zhang R Y. Molecular identification of sugarcane white leaf in Puer,Yunnan province,China.
Sugar Tech
|
CSCD被引
1
次
|
|
|
|
14.
Li W F. Occurrence and control strategies of quarantine disease sugarcane white leaf (in Chinese).
中国糖料,2014(3):66-68
|
CSCD被引
1
次
|
|
|
|
15.
Li W F. A quarantining sugarcane white leaf disease caused by phytoplasma found in sugarcane field in Yunnan (in Chinese).
植物病理学报,2014,44(5):556-560
|
CSCD被引
2
次
|
|
|
|
16.
Deng S. Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes.
Journal of Microbiological Methods,1991,14(1):53-61
|
CSCD被引
15
次
|
|
|
|
17.
Lee I. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences.
International Journal of Systematic Bacteriology,1998,48(4):1153-1169
|
CSCD被引
66
次
|
|
|
|
18.
Wangkeeree J. Candidates for symbiotic control of sugarcane white leaf disease.
Applied and Environmental Microbiology,2012,78(19):6804-6811
|
CSCD被引
3
次
|
|
|
|
19.
Ling K C. White leaf disease of sugarcane.
Taiwan Sugar,1962(9):1-5
|
CSCD被引
2
次
|
|
|
|
20.
Leu L S. An insectary method for testing sugarcane varieties for resistance to white leaf disease.
Proceedings of the XV International Society of Sugarcane Technologists Congress,1974:266-274
|
CSCD被引
2
次
|
|
|
|
|