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基于Dickeya dadantii特有标志基因检测甘薯茎腐病菌
Detection of stem and root rot pathogen of sweet potato based on conserved signature genes specific to Dickeya dadantii

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吴秀芹 1   罗金燕 2   孙国昌 3   易建平 4   李斌 1   安千里 1 *  
文摘 甘薯细菌性茎腐病是由达旦提狄克氏菌(Dickeya dadantii)引起的一种检疫性病害,近年来在我国多地发生,严重威胁我国甘薯产业的发展。建立特异灵敏的检测D. dadantii的方法对于鉴定检疫病原菌、田间监测病原菌和防控病害有重要意义。本研究对Dickeya属菌株的全基因组序列进行比较基因组学分析,筛选到D. dadantii特有的标志基因,针对标志基因设计引物,其中针对编码登录号为WP_077245517未知蛋白基因的1对引物Dad1-F(5'-CATATCAACCAGACCAGCCGTT-3')和Dad1-R(5'-CGGCCTGCTTTTAAACAACGTATTA-3')能只从D. dadantii扩增到167 bp目的片段。由此建立了特异灵敏的常规PCR和实时荧光定量PCR检测D. dadantii的方法,为鉴定检疫甘薯茎腐病病原菌和田间监测病害提供了有效方法。
其他语种文摘 Bacterial stem and root rot of sweet potato caused by Dickeya dadantii is a new quarantine plant disease in China. Recent outbreaks of this disease threaten sweet potato production in China. Specific and sensitive detection of D. dadantii is critical for the pathogen identification,distribution monitoring and the disease control. Here,conserved signature genes specific to D. dadantii were found by the whole genome sequences analysis of the strains belonging to the genus Dickeya. Primers targeting to the conserved signature genes were designed and tested by PCR. One set of primers Dad1-F (5'-CATATCAACCAGACCAGCCGTT-3') and Dad1-R (5'-CGGCCTGCTTTTAAACAACGTATTA-3'), which targets to a conserved signature gene encoding an unknown protein with the accession number WP_077245517,specifically amplified a 167 bp fragment from D. dadantii by conventional PCR and real-time fluorescent quantitative PCR,providing a specific and sensitive method for identification and monitoring of D. dadantii in the field.
来源 植物病理学报 ,2019,49(5):688-698 【核心库】
DOI 10.13926/j.cnki.apps.000298
关键词 软腐病 ; 甘薯茎腐病 ; 狄克氏菌 ; 保守标志蛋白 ; 种特异基因
地址

1. 浙江大学生物技术研究所, 农业部作物病虫分子生物学重点实验室;;浙江省作物病虫生物学重点实验室, 杭州, 310058  

2. 上海市农业技术推广服务中心, 上海, 201103  

3. 浙江省农业科学院植物保护与微生物研究所, 杭州, 310021  

4. 上海出入境检验检疫局, 上海, 200135

语种 中文
文献类型 研究性论文
ISSN 0412-0914
学科 植物保护
基金 浙江省重点研发项目 ;  浙江省自然科学基金 ;  上海市农业基础性研究项目
文献收藏号 CSCD:6697473

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引证文献 1 篇

1 张健男 利用实时荧光定量PCR和数字PCR检测鉴定水稻细菌性条斑病菌 浙江大学学报. 农业与生命科学版,2023,49(1):55-64
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