等离子体诱变灵芝选育高产纳米硒菌株
Plasma mutagenesis of Ganoderma lingzhi for breeding the high‐nanoselenium strains
查看参考文献30篇
|
文摘
|
本研究应用介质阻挡放电(DBD)等离子体对一种富硒灵芝的原生质体进行诱变,并筛选出高产纳米硒菌株。通过实验,得到优化的等离子体放电条件,如:电压15.6kV、电流1.8mA、放电频率1.8kHz、处理2.5min等,在此条件进行诱变处理,通过随机扩增多态性DNA标记(RAPD)法鉴定,筛选出6株突变菌株。对突变菌株进行传代培养,富硒培养测定菌丝体中硒含量发现突变菌株(H10)与出发菌株相比,菌丝体中纳米硒含量提高了大约30%,从而得到富硒的优良突变菌株。本研究为灵芝提供了一种方便可行的诱变方法,并为灵芝纳米硒相关产品的开发和应用提供了优良菌株。 |
|
其他语种文摘
|
Dielectric barrier discharge (DBD) plasma was applied to treat the protoplast of Ganoderma lingzhi for obtaining high‐nano‐selenium mutant. Under the condition of experimental parameters of voltage 15.6kV, current 1.8mA, discharge frequency 1.8kHz, and the treatment duration of 2.5min, the optimal effect of DBDP mutagenesis was obtained, and six mutants were screened by random amplified polymorphic DNA (RAPD) labeling approach. Multiple‐generation culture showed that mutant H10 produced the highest yield of nano‐selenium. Compared with the original strain, the selenium content in the mycelium of H10 increased by about 30%. This study proves that plasma mutagenesis seems feasible for obtaining excellent Ganoderma lingzhi mutant strains with the high‐yield of nano‐selenium. |
|
来源
|
菌物学报
,2020,39(1):13-22 【核心库】
|
|
DOI
|
10.13346/j.mycosystema.190297
|
|
关键词
|
灵芝
;
低温等离子体
;
诱变
;
纳米硒
|
|
地址
|
1.
中国科学院合肥物质科学研究院技术生物与农业工程研究所, 安徽, 合肥, 230031
2.
安徽科技学院农学院, 安徽, 凤阳, 233100
3.
合肥学院, 安徽, 合肥, 230601
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1672-6472 |
|
学科
|
农作物 |
|
基金
|
国家自然科学基金
|
|
文献收藏号
|
CSCD:6676765
|
参考文献 共
30
共2页
|
|
1.
Benko I. Subacute toxicity of nano-selenium compared to other selenium species in mice.
Environmental Toxicology and Chemistry,2012,31(12):2812-2820
|
被引
10
次
|
|
|
|
|
2.
Cao Y. Species clarification of the prize medicinal Ganoderma mushroom "Lingzhi".
Fungal Diversity,2012,56(1):49-62
|
被引
108
次
|
|
|
|
|
3.
Dhanjal S. Aerobic biogenesis of selenium nanospheres by Bacillus cereus isolated from coalmine soil.
Microbial Cell Factories,2010,9(1):52
|
被引
16
次
|
|
|
|
|
4.
Forootanfar H. Antioxidant and cytotoxic effect of biologically synthesized selenium nanoparticles in comparison to selenium dioxide.
Journal of Trace Elements in Medicine & Biology Organ of the Society for Minerals & Trace Elements,2014,28(1):75-79
|
被引
6
次
|
|
|
|
|
5.
He Y. Toxicity of selenium nanoparticles in male Sprague-Dawley rats at supranutritional and nonlethal levels.
Life Sciences,2014,115(1/2):44-51
|
被引
4
次
|
|
|
|
|
6.
Jia X. A subchronic toxicity study of elemental nano-Se in Sprague-Dawley rats.
Life Sciences,2005,76(17):1989-2003
|
被引
16
次
|
|
|
|
|
7.
Jiang S H. Mercury capture into biogenic amorphous selenium nanospheres produced by mercury resistant Shewanella putrefaciens 200.
Chemosphere,2009,74(4):621-624
|
被引
1
次
|
|
|
|
|
8.
Nie S. Current development of polysaccharides from Ganoderma: isolation, structure and bioactivities.
Bioactive Carbohydrates and Dietary Fibre,2013,1(1):10-20
|
被引
10
次
|
|
|
|
|
9.
Poluboyarinov P A. Elemental selenium formation upon destruction of the organoselenium compound DAFS-25 molecule by growing fungal mycelium.
Moscow University Biological Sciences Bulletin,2009,64(4):164-168
|
被引
1
次
|
|
|
|
|
10.
Su C X. Mutation breeding of chitosanase-producing strain Bacillus sp. S65 by low-energy ion implantation.
Journal of Industrial Microbiology & Biotechnology,2006,33(12):1037-1042
|
被引
9
次
|
|
|
|
|
11.
Wangeline A L. Characterization of rhizosphere fungi from selenium hyperaccumulator and nonhyperaccumulator plants along the eastern Rocky Mountain Front Range.
American Journal of Botany,2011,98(7):1139-1147
|
被引
1
次
|
|
|
|
|
12.
Wang T. Extracellular biosynthesis and transformation of selenium nanoparticles and application in H_2O_2 biosensor.
Colloids & Surfaces Biointerfaces,2010,80(1):94-102
|
被引
5
次
|
|
|
|
|
13.
Wang Y. Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities.
Bioorganic & Medicinal Chemistry,2002,10(4):1057-1062
|
被引
2
次
|
|
|
|
|
14.
Zeng H. Selenium as an anticancer nutrient: roles in cell proliferation and tumor cell invasion.
The Journal of Nutritional Biochemistry,2008,19(1):1-7
|
被引
8
次
|
|
|
|
|
15.
Zhang J S. Comparison of short-term toxicity between nano-Se and selenite in mice.
Life Sciences,2005,76(10):1099-1109
|
被引
13
次
|
|
|
|
|
16.
Zhang J S. Elemental selenium at nano size (nano-Se) as a potential chemopreventive agent with reduced risk of selenium toxicity: comparison with se-methylselenocysteine in mice.
Toxicological Sciences,2008,101(1):22-31
|
被引
8
次
|
|
|
|
|
17.
Zhao L. Selenium distribution in a Se-enriched mushroom species of the genus Ganoderma.
Journal of Agricultural and Food Chemistry,2004,52(12):3954-3959
|
被引
16
次
|
|
|
|
|
18.
戴玉成. 中国灵芝学名之管见.
菌物学报,2013,32(6):947-952
|
被引
100
次
|
|
|
|
|
19.
龚定芳. 常压室温等离子体诱变选育DHA高产菌株.
中国油脂,2019,44(3):118-122
|
被引
5
次
|
|
|
|
|
20.
林志彬. 灵芝抗肿瘤作用的免疫学机制及其临床应用.
中国药理学与毒理学杂志,2015,29(6):865-882
|
被引
12
次
|
|
|
|
|
了解气象卫星更多信息，请访问