携带IL-10基因的双歧杆菌对感染后肠易激综合征的治疗作用
The therapeutic effect of Bifidobacterium expressing human IL-10 on post-infectious irritable bowel syndrome
查看参考文献8篇
|
文摘
|
目的探讨转基因双歧杆菌对感染后肠易激综合征(PI-IBS)小鼠的治疗作用。方法50只成年雄性SD小鼠,随机选取10只作为空白对照组(NC组),其余40只PI-IBS模型小鼠随机分为IBS组(IBS组)、双歧杆菌治疗组(BL组)、空质粒双歧杆菌治疗组(BL-0组)和BL-hIL-10治疗组(每组10只)。NC组和IBS组每天予0.2 mL PBS灌胃1次;其余三组每天灌胃溶有经0.2% L-Arb诱导24 h后的BL、BL-0、BL-hIL-10菌液(双歧杆菌浓度达5×10~8个/mL)的PBS液0.2 mL,每天1次。所有小鼠干预7 d后处死,然后行血液及结肠组织学检验。结果(1)通过评估小鼠疾病活动指数、小鼠脾脏指数和结肠组织HE染色病理学变化,证明口服BL-hIL-10能显著减轻PI-IBS小鼠的炎症症状;(2)BL-hIL-10能升高IL-10比例并降低IL-12的比例,维持Th平衡。结论口服转基因双歧杆菌可以缓解PI-IBS小鼠的炎症反应,其机制可能是通过调节Th平衡来实现。 |
|
其他语种文摘
|
Objective To explore the possible therapeutic effect of BL-hIL-10 for post-infectious irritable bowel syndrome (PI-IBS). Methods 50 mice were randomly divided into five groups (n=10): normal control (NC) group, IBS group, BL group, BL-0 group and BL-hIL-10 group.All the mice were given 0.2 mL of phosphate buffered saline (PBS) containing none or differently treated Bifidobacteria (BL, BL-0 and BL-hIL-10; 5×10~8).After seven days of treatment, the specimens of blood, spleens and colons of mice were collected for histological detection. Results (1) BL-hIL-10 treatment reduced the DAI and the degree of inflammation in PI-IBS mice.(2) After BL-hIL-10 treatment, the proportion of IL-10 increased while that of IL-12 reduced, leading to the reconstruction of Th balance. Conclusion Oral administration of BL-hIL-10 can alleviate the inflammation responses of PI-IBS in murine models possibly through the restoration of Th balance. |
|
来源
|
中国微生态学杂志
,2019,31(2):138-140,145 【扩展库】
|
|
DOI
|
10.13381/j.cnki.cjm.201902003
|
|
关键词
|
肠易激综合征
;
双歧杆菌
;
IL-10
|
|
地址
|
深圳市人民医院,南方科技大学附属第一医院, 暨南大学第二临床医学院消化内科, 广东, 深圳, 518020
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1005-376X |
|
学科
|
内科学 |
|
基金
|
深圳市卫生计生系统科研项目
;
广东省医学科学技术研究基金
;
深圳市人民医院三名合作科研培育项目
|
|
文献收藏号
|
CSCD:6436420
|
参考文献 共
8
共1页
|
|
1.
Liebregts T. Immune activation in patients with irritable bowel syndrome.
Gastroenterology,2007,132(3):913-920
|
CSCD被引
27
次
|
|
|
|
|
2.
Yao J. Treatment of mice with dextran sulfate sodium-induced colitis with human interleukin 10 secreted by transformed Bifidobacterium longum.
Mol Pharm,2011,8(2):488-497
|
CSCD被引
6
次
|
|
|
|
|
3.
Zhang D. Interleukin-10 gene-carrying bifidobacteria ameliorate murine ulcerative colitis by regulating regulatory T cell/T helper 17 cell pathway.
Exp Biol Med,2015,240(12):1622-1629
|
CSCD被引
6
次
|
|
|
|
|
4.
张定国. 携带IL-10基因双歧杆菌载体的构建及鉴定.
中国基层医药,2015,22(8):1136-1138
|
CSCD被引
3
次
|
|
|
|
|
5.
龙艳芹. 感染后肠易激综合征模型小鼠肠道组织中Th1/Th2的漂移.
中华消化杂志,2010,30(1):24-27
|
CSCD被引
1
次
|
|
|
|
|
6.
Spiller R. Postinfectious irritable bowel syndrome.
Gastroenterology,2009,136(6):1979-1988
|
CSCD被引
22
次
|
|
|
|
|
7.
O'Mahony L. Lactobacillus and Bifidobacterium in irritable bowel syndrome: Symptom responses and relationship to cytokine profiles.
Gastroenterology,2005,128(3):541-551
|
CSCD被引
39
次
|
|
|
|
|
8.
Gonsalkorale W M. Interleukin 10 genotypes in irritable bowel syndrome: Evidence for an inflammatory component?.
Gut,2003,52(1):91-93
|
CSCD被引
13
次
|
|
|
|
|
了解气象卫星更多信息，请访问