微流控芯片在单细胞捕获中的应用
Microfluidic chips for single-cell trapping
查看参考文献35篇
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文摘
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单细胞捕获是单细胞水平研究的前提和重要组成部分。微流控芯片通常具有与细胞尺寸相当的微通道结构,并能操控纳升至皮升级的极小体积流体,非常适用于高通量的单细胞捕获,加上微流控芯片能够将其他多种操作单元集成在一起,为单细胞分析提供了一种效率高、消耗低的研究平台。概述并对比了多种涉及流体力学、光、电、磁、声等领域的微流控单细胞捕获技术的原理和应用,展望了其未来的研究方向。 |
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其他语种文摘
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In recent years, the application of the microfluidics in the single cell analysis has attracted more and more attention. The singlecell trapping is the basic and key component for the analysis at the single-cell level. The microfluidic chips usually have single-cell matched microstructures and could achieve the control of extremely small liquid volumes at the nanoliter and picoliter scale. Thus, the microfluidic chips are particularly suitable for the specific or high-throughput single-cell trapping. Moreover, the components for the posttrapping analysis of single cells could be integrated on the chip to build a high-efficiency and low-cost microfluidic single-cell analysis platform. This paper reviews and compares several microfluidic single-cell trapping methods, including hydrodynamic, optical, electrical, magnetic, and acoustic techniques. Future research in the single-cell trapping and analysis on the microfluidic chips is also discussed. |
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来源
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科技导报（北京）
,2018,36(16):39-45 【扩展库】
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DOI
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10.3981/j.issn.1000-7857.2018.16.004
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关键词
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微流控芯片
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单细胞捕获
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液滴微流控
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介电电泳
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光镊
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地址
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1.
南方科技大学材料科学与工程系, 深圳, 518055
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南方科技大学前沿与交叉科学研究院, 深圳, 518055
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-7857 |
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学科
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电子技术、通信技术 |
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基金
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深圳市科创委基础研究项目
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文献收藏号
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CSCD:6318124
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