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帕金森病患者肠道菌群变化的研究
Structural changes of gut microbiota in patients with Parkinson's disease

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赵程 1   于会艳 1   李薇 2   时晶 3   秦斌 1 *  
文摘 目的探究帕金森病患者肠道菌群的状况。方法选择2015年来北京医院就诊的帕金森病患者24例作为帕金森病组,同时选择14名年龄、性别匹配的健康居民作为健康对照组。采集受试者一般临床资料,采用帕金森病相关量表对所有受试者进行评估,采集粪便后提取DNA并进行细菌16S rRNA高通量测序,对两组之间菌群状况进行分析。结果在门水平上,放线菌门在帕金森病组相对丰度明显高于健康对照组[分别为0.76% (0.13%,1.85%)、0.14% (0.07% ,0.30%),Z = 2.784,P <0.01],而在拟杆菌门中帕金森病组的菌群相对丰度却显著降低[分别为57.28% (48.75% ,64.95%)、63.78% (56.72%,68.21%) ,Z = -4.963,P <0.01]。在纲水平上,芽孢杆菌纲[分别为0.52% (0.11% ,2.10%)、0.13% (0.05%,0.16%) ,Z = 2.693 <0.01]、革兰阴性菌纲[分别为5.04% (2.93% ,14.02%)、2.87% (1.46% ,4.43%) ,Z = 2.360,P = 0.018]、放线菌纲[分别为0.60% (0.10%,1.59%)、0.12% (0.04% , 0.20%), Z = 2.512, P = 0.011]、γ-变形菌纲[分别为1.72% (0.58% ,5.46%)、0.43% (0.24% ,2.19%) ,Z = 2.179,P = 0.029]在帕金森病组的相对丰度明显高于健康对照组。在科水平上,韦荣球菌科[分别为3.78% (0.53% ,13.82%)、0.49% (0.08% , 3.14%),Z = 2.754,P<0.01]、链球菌科[分别为0.33% (0.09% ,0.69%)、0.19% (0.14% ,0.24%), Z = 1.770,P = 0.004]、肠杆菌科[分别为1.04% (0.40% ,4.95%), 0.20% (0.10% ,0.45%),Z = 2.784,P<0.01]、乳杆菌科[分别为0.079% (0.014%,0.575%)、0.003% (0.002% ,0.028%),Z = 3.119,P <0.01]、双歧杆菌科[分别为0.60% (0.09%,1.57%)、0.11% (0.03% ,0.19%) ,Z = 2.481, P=0.012]在帕金森病组的相对丰度均有明显增高;而巴斯德杆菌科[分别为0.009% (0.002%, 0.047%)、0.110% (0.022% ,0.898%),Z= -2.545,P = 0.010]在帕金森病组中相对丰度却明显低于健康对照组。结论帕金森病组和健康对照组中菌群结构在门、纲、科水平上均有明显的差异。这些变化可能与帕金森病的发生发展有关。
其他语种文摘 Objective To investigate the structural changes of gut microbiota in patients with Parkinson's disease (PD) . Methods Twenty-four PD patients and 14 healthy controls from Beijing Hospital in 2015 were recruited in this cross-sectional study. The general clinical information was collected and all subjects were assessed with Parkinson's disease related scales. The gut microbiota status between two groups was analyzed after extracting feces' DNA and carrying out high-throughput sequencing of bacterial 16S rRNA. Results At the phylum level, actinobacteria (0.76% (0.13% , 1.85%) vs 0.14% (0.07%, 0.30%), Z = 2.784, P< 0.01) were significantly increased and bacteroidetes (57.28% (48.75% , 64.95%) vs 63.78% (56.72% , 68.21%), Z= -4.963, P<0.01) were significantly decreased in PD patients compared to healthy controls. At the class level, bacilli (0.52% (0.11%,2.10%) vs 0.13% (0.05% , 0.16%) , Z=2.693 , P< 0.01) , negativicutes (5.04% (2.93% , 14.02%) vs 2.87% (1.46% , 4.43%), Z= 2.360, P= 0.018) , actinobacteria (0.60% (0.10%, 1.59%) vs 0.12% (0.04%, 0.20%), Z= 2.512, P=0.011), gammaproteobacteria (1.72% (0.58%, 5.46%) vs 0.43% (0.24%,2.19%), Z = 2.179, P = 0.029) were significantly increased in PD patients compared to healthy controls. At the family level, veillonellaceae (3.78% (0.53%,13.82%) vs 0.49% (0.08%, 3.14%), Z = 2.754, P<0.01), streptococcaceae (0.33% (0.09%,0.69%) vs 0.19% (0.14%, 0.24%), Z = 1.770, P = 0.004), enterobacteriaceae (1.04% (0.40%,4.95%) vs 0.20% (0.10%, 0.45%), Z= 2.784, P< 0.01), lactobacillaceae (0.079% (0.014%,0.575%) vs 0.003% (0.002%, 0.028%), Z = 3.119, P<0.01), bifidobacteriaceae (0.60% (0.09%,1.57%)vs 0.11 % (0.03%,0.19%),Z = 2.481,P=0.012) were significantly increased and pasteurellaceae (0.009% (0.002%,0.047%) vs 0.110% (0.022%,0.898%),Z= - 2.545, P= 0.010) were significantly decreased in PD patients compared to healthy controls. Conclusions The structures of gut microbiota in PD patients and healthy controls were significantly different at the levels of phylum, class,and family. All these changes are potentially associated with the development of PD pathology.
来源 中华神经科杂志 ,2018,51(7):498-503 【核心库】
DOI 10.3760/cma.j.issn.1006-7876.2018.07.004
关键词 帕金森病 ; 肠道菌群 ; 高通量核苷酸测序 ; 肠道菌群紊乱
地址

1. 北京医院神经内科,国家老年医学中心, 100730  

2. 中国科学院心理研究所, 中国科学院心理健康重点实验室  

3. 北京中医药大学东直门医院脑病三科

语种 中文
文献类型 研究性论文
ISSN 1006-7876
学科 医药、卫生
基金 首都卫生发展科研专项
文献收藏号 CSCD:6272938

参考文献 共 20 共1页

1.  Braak H. Staging of brain pathology related to sporadic Parkinson's disease. Neurobiol Aging,2003,24(2):197-211 CSCD被引 240 次    
2.  Rietdijk C D. Exploring Braak's Hypothesis of Parkinson's Disease. Front Neurol,2017,8:37 CSCD被引 6 次    
3.  Braak H. Neuropathological Staging of Brain Pathology in Sporadic Parkinson's disease: Separating the Wheat from the Chaff. J Parkinsons Dis,2017,7(s1):S71-S85 CSCD被引 17 次    
4.  Schepeijans F. Gut microbiota are related to Parkinson's disease and clinical phenotype. Mov Disord,2015,30(3):350-358 CSCD被引 108 次    
5.  Gibb W R. The relevance of the Lewy body to the pathogenesis of idiopathic Parkinson's disease. J Neurol Neurosurg Psychiatry,1988,51(6):745-752 CSCD被引 47 次    
6.  Blaxter M. Defining operational taxonomic units using DNA barcode data. Philos Trans R Soc Lond B Biol Sci,2005,360(1462):1935-1943 CSCD被引 97 次    
7.  Tuomisto H. A diversity of beta diversities: straightening up a concept gone awry. Part 1. Defining beta diversity as a function of alpha and gamma diversity. Ecography,2010,33(1):2-22 CSCD被引 44 次    
8.  Kadam P D. Erratum to: Rectocutaneous fistula with transmigration of the suture: a rare delayed complication of vault fixation with the sacrospinous ligament. Int Urogynecol J,2016,27(3):505 CSCD被引 20 次    
9.  Nakamura A. Relationship between urinary sodium excretion and pioglitazone-induced edema. J Diabetes Investig,2010,1(5):208-211 CSCD被引 31 次    
10.  Shannon K M. Alpha-synuclein in colonic submucosa in early untreated Parkinson's disease. Mov Disord,2012,27(6):709-715 CSCD被引 21 次    
11.  Bencsik A. Early and persistent expression of phosphorylated alpha-synuclein in the enteric nervous system of A53T mutant human alpha-synuclein transgenic mice. J Neuropathol Exp Neurol,2014,73(12):1144-1151 CSCD被引 2 次    
12.  Keshavarzian A. Colonic bacterial composition in Parkinson's disease. Mov Disord,2015,30(10):1351-1360 CSCD被引 50 次    
13.  German J B. Saturated fats: what dietary intake?. Am J Clin Nutr,2004,80(3):550-559 CSCD被引 5 次    
14.  Vinolo M A. Regulation of inflammation by short chain fatty acids. Nutrients,2011,3(10):858-876 CSCD被引 68 次    
15.  Unger M M. Short chain fatty acids and gut microbiota differ between patients with Parkinson's disease and age-matched controls. Parkinsonism Relat Disord,2016,32:66-72 CSCD被引 53 次    
16.  Cavanagh J B. The neurotoxin of Shigella shigae ; a comparative study of the effects produced in various laboratory animals. Br J Exp Pathol,1956,37(3):272-278 CSCD被引 1 次    
17.  Huycke M M. Enterococcus faecalis produces extracellular superoxide and hydrogen peroxide that damages colonic epithelial cell DNA. Carcinogenesis,2002,23(3):529-536 CSCD被引 20 次    
18.  Schaffer J N. Proteus mirabilis and Urinary Tract Infections. Microbiol Spectr,2015,3(5) CSCD被引 8 次    
19.  Al Iliev. Rapid microtubule bundling and stabilization by the Streptococcus pneumoniae neurotoxin pneumolysin in a cholesterol-dependent, non-lytic and Src-kinase dependent manner inhibits intracellular trafficking. Mol Microbiol,2009,71(2):461477 CSCD被引 1 次    
20.  Sampson T R. Gut Microbiota Regulate Motor Deficits and Neuroinflammation in a Model of Parkinson's Disease. Cell,2016,167(6):1469-1480 CSCD被引 167 次    
引证文献 4 篇

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