闽楠叶斑病病原鉴定及其生物学特性测定
Pathogen identification of Phoebe bournei leaf spot and determination of its biological characteristics
查看参考文献31篇
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文摘
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闽楠是我国特有珍稀优良用材乡土树种,市场前景广阔。叶斑病是闽楠推广种植过程中新出现的叶部病害,可影响树木正常生长。闽楠叶斑病病原鉴定及生物学特性等研究,有助于明确病原分类地位和病害发生流行规律,从而科学有效地指导防治。通过形态学特征观测和rDNA-ITS序列分析,确定闽楠叶斑病病原为小孢拟盘多毛孢(Pestalotiopsis microspora)。生物学特性测定结果表明:闽楠小孢拟盘多毛孢ZZ-03菌株以Czapek或PDA+PBL培养基(pH 6.0) 28℃黑暗恒温培养有利于菌丝生长;以PDA培养基(pH 8.0) 28℃黑暗恒温培养有利于菌株产孢;以pH 8.0、28℃、全黑暗有利于分生孢子萌发;以葡萄糖为C源、牛肉浸膏为N源有利于菌丝生长,以葡萄糖为C源、蛋白胨为N源有利于产孢;分生孢子萌发与相对湿度呈正相关。 |
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其他语种文摘
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Phoebe bournei,one of the national second-class endangered plants,is the excellent precious timer species with broad market prospect.Leaf spot was newly found leaf disease,which resulted in stress and abnormal growth of plants in the extension of Ph.bournei plantation.The causing agent of the disease was identified as Pestalotiopsis microspora by morphological characteristics and rDNA-ITS sequences analysis.The result of the biological test showed that the optimal conditions for mycelia growth of Pe.microspora strain ZZ-03 were Czapek agar or PDA+PBL(pH 6.0) at 28℃ in dark.The spore production was optimal on PDA medium(pH 8.0) at 28℃ in dark.Conidia germination was positively correlated with relative humidity at 28℃ with pH 8.0 in dark.The best carbon and nitrogen sources for mycelia growth were glucose and beef extract,respectively,while glucose and peptone were the best for conidiation,respectively. |
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来源
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植物病理学报
,2018,48(3):313-323 【核心库】
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DOI
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10.13926/j.cnki.apps.000133
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关键词
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闽楠
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叶斑病
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病原鉴定
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小孢拟盘多毛孢
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生物学特性
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地址
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1.
福建农林大学金山学院, 福州, 350002
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福建农林大学林学院, 福州, 350002
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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林业 |
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基金
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福建省教育厅中青年教师教育科研项目
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福建农林大学金山学院科研启动经费
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福建省财政厅项目
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文献收藏号
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CSCD:6260765
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参考文献 共
31
共2页
|
|
1.
Zeng Z H. Development strategy of Phoebe trees base in Ganzhou City of Jiangxi Province (in Chinese).
南方林业科学,2015,43(5):55-57
|
CSCD被引
1
次
|
|
|
|
|
2.
Zhu L. Research on resistance physiology of Phoebe bournei and Phoebe nanmu under the saline-alkaline stress (in Chinese).
湖北林业科技,2015,44(5):14-16,72
|
CSCD被引
2
次
|
|
|
|
|
3.
Chu X L. 不同生境、造林模式闽楠人工林生长及林分分化.
林业科学研究,2014,27(4):445-453
|
CSCD被引
20
次
|
|
|
|
|
4.
Wang Z X. 闽楠幼树光合特性及生物量分配对光环境的响应.
生态学报,2012,32(12):3841-3848
|
CSCD被引
61
次
|
|
|
|
|
5.
Huang X R. Effect of water stress on growth and physiological characteristics of Phoebe zhennan S. Lee et F. N. Wei (in Chinese).
北方园艺,2015(7):68-71
|
CSCD被引
7
次
|
|
|
|
|
6.
Sheng J. 闽楠家系苗期光合特性的研究.
中南林业科技大学学报,2015,35(6):45-49
|
CSCD被引
6
次
|
|
|
|
|
7.
Wang D G. 氮素营养对闽楠幼苗生长及光合特性的影响.
林业科学研究,2013,26(1):70-75
|
CSCD被引
21
次
|
|
|
|
|
8.
Ren W J. Study on chemical components of the essential oil from the peel of Phoebe zhennan (in Chinese).
天然产物研究与开发,1990,2(3):59-62
|
CSCD被引
5
次
|
|
|
|
|
9.
Liu B. 21个闽楠种源叶片光合色素含量及叶绿素荧光参数分析.
江西农业大学学报,2014,36(1):115-121
|
CSCD被引
12
次
|
|
|
|
|
10.
Guan B. Identification of pathogen for leaf spot disease of Photinia fraseri and pathogenicity test (in Chinese).
西部林业科学,2013,42(2):56-61
|
CSCD被引
8
次
|
|
|
|
|
11.
Chen Q Z. 福建省桉树焦枯病菌分类鉴定.
福建林学院学报,2013,33(2):176-182
|
CSCD被引
13
次
|
|
|
|
|
12.
Cui C Y. Identification of the pathogen causing brown spot disease of Acer rubrum'October Glory'(in Chinese).
林业科学,2015,51(10):142-147
|
CSCD被引
1
次
|
|
|
|
|
13.
Qu W W. 山东省危害核桃的链格孢属真菌鉴定及其系统发育.
植物保护学报,2012,39(2):121-128
|
CSCD被引
18
次
|
|
|
|
|
14.
Li X X. Identification and the internal transcribed spacer region analysis of pathogen causing loquat anthracnose in Guizhou (in Chinese).
中国森林病虫,2012,31(2):7-10
|
CSCD被引
1
次
|
|
|
|
|
15.
Fang Z D.
Research methods for plant disease (2rd Ed.) (in Chinese),1998
|
CSCD被引
1
次
|
|
|
|
|
16.
Chen Q Z. 桉树焦枯病菌(Calonectria pseudoreteaudii)生物学特性测定.
福建林学院学报,2014,34(4):328-332
|
CSCD被引
10
次
|
|
|
|
|
17.
Chen N Q. Biological characteristics of Pestalotiopsis microspora (in Chinese).
保鲜与加工,2016,16(3):5-10
|
CSCD被引
9
次
|
|
|
|
|
18.
Zhao G C. Thirty-four species of Pestalotiopsis in Yunnan (in Chinese).
东北林业大学学报,1995,23(4):21-27
|
CSCD被引
10
次
|
|
|
|
|
19.
Ge Q X.
Flora fungorum sinicorum (Vol. 38) (in Chinese),2009
|
CSCD被引
2
次
|
|
|
|
|
20.
Yu J Y. 石楠叶斑病病原鉴定及对药物敏感性测定.
植物病理学报,2017,47(4):440-447
|
CSCD被引
7
次
|
|
|
|
|
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