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锐顶镰孢致病性及苜蓿品种间的抗病性研究
Study on the pathogenicity of Fusarium acuminatum and disease resistance of alfalfa varieties

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郭玉霞 1   郭志鹏 1   张靖雪 1   王苗利 1   曲根 1   严学兵 1 *   张猛 2 *  
文摘 为了探明锐顶镰孢菌对接种后苜蓿种苗的致病性,本研究采用菌饼接种法,将分离自4个地点的15个锐顶镰孢菌株接种于14个紫花苜蓿品种的种子上,接种后14 d测定相对根长、苗高、发芽率,并计算病情指数.结果表明,参试的15个菌株均显著降低了苜蓿种苗的根长(P<0.05).总体来看,各菌株之间的致病性有所不同,从苜蓿品种WL-323HQ中分离出来的5号菌株的致病性最强,从WL-525HQ中分离出来的13号菌株的致病性最弱,从WL-525HQ、Legacy、Affinity和Millionaire中分离出来的12号、10号、8号和6号菌株的致病性较强,从Aohan、Farmers、WL-323ML和Dingdian中分离出来的1号、4号、7号和9号菌株的致病性较弱;参试的14个苜蓿品种之间的抗病性也各不相同,Amerimultileaf的抗病性最强,Amerileaf721、Victoria、Powerplant和Legacy的抗病性较强,Sandy、Phabulous、Hunter river和Alfaking的抗病性较弱, Vernal的抗病性最弱.
其他语种文摘 The objective of this study was to evaluate the pathogenicity of Fusarium acuminatum isolates to various alfalfa varieties. Fourteen varieties of alfalfa were inoculated with 15 F. acuminatum isolates obtained from alfalfa roots collected at 4 locations. The relative root length,relative seedling height,relative germination rate and disease index were assayed at 14 days after inoculation. The results indicated that the root length of alfalfa seedlings was significantly reduced by all of the 15 isolates (P<0.05). Overall,the pathogenicity of each isolate varied among the 15 isolates. The isolate from‘WL-323HQ'exhibited the strongest pathogenicity,while the isolate from‘WL-525HQ'(planted in experimental area of Neihuang County of Anyang City )showed the weakest pathogenicity. The isolates from ‘WL-525HQ'(planted in experimental area in Zhengzhou City ), ‘Legacy',‘Affinity'and ‘Millionaire'showed strong pathogenicity,while the isolates from ‘Aohan', ‘Farmers',‘WL-323ML'and‘Dingdian'showed weak pathogenicity. The disease resistance of the 14 alfalfa varieties also varied,among of them,‘Amerimultileaf'showed the strongest resistance. ‘Amerileaf721', ‘Victoria',‘Powerplant'and ‘Legacy'exhibited high-level resistance. ‘Sandy',‘Phabulous',‘Hunter river'and‘Alfaking'showed low-level resistance.‘Vernal'had the weakest resistance.
来源 植物病理学报 ,2018,48(2):213-222 【核心库】
DOI 10.13926/j.cnki.apps.000129
关键词 紫花苜蓿 ; 锐顶镰孢 ; 根腐病 ; 致病性 ; 抗病性
地址

1. 河南农业大学牧医工程学院, 郑州, 450002  

2. 河南农业大学植物保护学院, 郑州, 450002

语种 中文
文献类型 研究性论文
ISSN 0412-0914
学科 植物保护
基金 国家自然科学基金项目 ;  河南省高等学校重点科研项目 ;  国家公益行业项目
文献收藏号 CSCD:6220425

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引证文献 7

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