应用real-time PCR评价三种杀菌剂对小麦条锈病的防治效果
Efficacy evaluation of three kinds of fungicides on wheat stripe rust using real-time PCR
查看参考文献21篇
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文摘
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为更加精确有效地评估杀菌剂对小麦条锈病的防效,本研究利用real-time PCR检测潜育阶段的分子病情指数(molecular disease index,MDI)并结合田间病情分析,对三种杀菌剂的防效进行评价。春季返青后在品种铭贤169和京0045试验小区的诱发中心接种小麦条锈菌夏孢子,待充分发病后将其铲除。设置播种前2%立克秀拌种,接种后21和28 d分别喷施25%阿米西达和30%苯甲-丙环唑1 500倍液三种处理。发病后调查不同处理的普遍率和严重度,计算病情指数和AUDPC 。结果表明,阿米西达和苯甲-丙环唑对小麦条锈病具有良好的防效且能够明显抑制发病中心的扩展,而经立克秀拌种对该病害的防治效果和发病中心扩展的抑制作用均不理想。对京0045于发病中心接种后24和33 d采样,经双重real-time PCR定量检测,计算MDI。结果显示潜育检测获得的MDI与AUDPC之间均具有极显著的相关性;阿米西达和苯甲-丙环唑对潜育期小麦条锈菌的扩展具有明显的持续抑制作用,而立克秀拌种仅对小麦条锈菌潜伏初期的扩展表现出一定的抑制作用。 |
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其他语种文摘
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For earlier and more accurate assessment efficacy of three kinds of fungicides on wheat stripe rust, the molecular disease index(MDI) of latent period obtained by real-time PCR and field actual disease were analyzed in this study. The induction centers among wheat cultivars Mingxian 169 and Jing 0045 experimental plots were inoculated with urediniospore of Puccinia striiformis f. sp. tritici(Pst) during the returning green-jointing stage and eradicated when these plants showed disease fully. Seed treatment with 2% Tebuconazole was conducted before sowed. 25% Azoxystrobin and 30% difenoconazole propiconazole were sprayed at 21 and 28 d after inoculation,respectively. Disease incidence and severity of different treatments were assessed,and disease index(DI),area under disease development curve(AUDPC) were calculated. The results revealed that plots treated with azoxystrobin and difenoconazole·propiconazole showed light symptoms of wheat stripe rust and disease development was restricted within certain ranges,while Tebuconazole treated plots showed severe symptoms with larger ranges. Samples of wheat cultivar Jing 0045 were collected at 24 and 33 d after the induction centers inoculation, respectively. Duplex real-time PCR was applied to quantify and MDI was obtained. The results indicated that the regressions between MDI and AUDPC were significant; Azoxystrobin and difenoconazole propiconazole had continued inhibition effect to Pst in latent period,while seed treatment of Tebuconazole showed inhibition effect just at the beginning of latent period. |
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来源
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植物病理学报
,2017,47(4):532-540 【核心库】
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DOI
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10.13926/j.cnki.apps.000059
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关键词
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小麦条锈病
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药效评价
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双重real-time PCR
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地址
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中国农业大学植物病理学系, 农业部植物病理学重点开放实验室, 北京, 100193
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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国家自然科学基金
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国家重点研发计划
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文献收藏号
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CSCD:6088560
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参考文献 共
21
共2页
|
|
1.
Wan A M. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002.
Plant Disease,2004,88(8):896-904
|
CSCD被引
164
次
|
|
|
|
|
2.
Li Z Q.
The rust disease in China (in Chinese),2001
|
CSCD被引
2
次
|
|
|
|
|
3.
Zhang P Z. Progress of research on fungicide triadimefon in agriculture of China (in Chinese).
浙江科技学院学报,2004,16(1):28-33
|
CSCD被引
2
次
|
|
|
|
|
4.
Bai X. Research and application of triazoles (in Chinese).
化学研究与应用,2007,19(7):721-729
|
CSCD被引
1
次
|
|
|
|
|
5.
Li X Z. Research advances on triazole compounds bearing biological activities (in Chinese).
农药,2003,42(1):4-5
|
CSCD被引
1
次
|
|
|
|
|
6.
Liu C D. Research advances in triazole fungicides and used to control wheat diseases (in Chinese).
山东农业大学学报(自然科学版),2005,36(1):157-160
|
CSCD被引
1
次
|
|
|
|
|
7.
Cao X R. Monitoring of resistance of Blumeria graminis f. sp. tritici isolates to triadimefon in 2007 (in Chinese).
植物保护,2008,34(6):74-77
|
CSCD被引
5
次
|
|
|
|
|
8.
Mumford R A. Detection of potato mop top virus and tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay.
Phytopathology,2000,90(5):448-453
|
CSCD被引
12
次
|
|
|
|
|
9.
Vandemark G J. QuantifyingPhytophthora medicaginis in susceptible and resistant alfalfa with a real-time fluorescent PCR assay.
Journal of Phytopathology,2003,151(11/12):577-583
|
CSCD被引
4
次
|
|
|
|
|
10.
Jackson E J. A quantitative assay of Puccinia coronata f. sp. avenae DNA in Avena sativa.
Plant Disease,2006,90(5):629-636
|
CSCD被引
1
次
|
|
|
|
|
11.
Aljawasim B. Evaluation of polymerase chain reaction (PCR) -based methods for rapid,accurate detection and monitoring of Verticillium dahliae in woody hosts by Real-time PCR.
Plant Disease,2015,99(6):866-873
|
CSCD被引
2
次
|
|
|
|
|
12.
Pan J J. Quantification of latent infections of wheat stripe rust by using Real-time PCR (in Chinese).
植物病理学报,2010,40(5):504-510
|
CSCD被引
5
次
|
|
|
|
|
13.
Yan J H. Quantification of latent infection of wheat stripe rust in the fields using Realtime PCR (in Chinese).
植物病理学报,2011,41(6):618-625
|
CSCD被引
4
次
|
|
|
|
|
14.
Pan Y. Study on relationship between the quantity of Puccinia striiformis f. sp. tritici in latent during overwinter and the disease index in Xiangyang area (in Chinese).
植物病理学报,2016,46(5):679-685
|
CSCD被引
1
次
|
|
|
|
|
15.
Guo J R. AReal-time PCR assay for quantitative and accurate assessment of fungicide eects on Mycosphaerella graminicola leaf blotch.
Journal of Phytopathology,2007,155(8):482-487
|
CSCD被引
1
次
|
|
|
|
|
16.
Enjalbert J. Isolation of twelve microsatellite loci,using an enrichment protocol, in the phytopathogenic fungus Puccinia striiformis f. sp. tritici.
Molecular Ecology,2002,2(4):563-565
|
CSCD被引
33
次
|
|
|
|
|
17.
Pan Y. Establishment and application of duplex Real-time PCR quantitative determination method on latent infection of wheat stripe rust (in Chinese).
植物病理学报,2016,46(4):485-491
|
CSCD被引
2
次
|
|
|
|
|
18.
Chen W Q. Integrated management of diseases and insert pests on wheat (in Chinese).
植物保护,2013,39(5):16-24
|
CSCD被引
2
次
|
|
|
|
|
19.
Bai Y L. The progresses of research and development on strobilurin fungicides (in Chinese).
农药,2007,46(5):289-296
|
CSCD被引
1
次
|
|
|
|
|
20.
Xie B H. Study on the technique of controlling powdery mildew of cucumber with amistar (in Chinese).
安徽农业科学,2011,39(3):1421-1423
|
CSCD被引
1
次
|
|
|
|
|
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