帮助 关于我们

返回检索结果

基于皮质酮-炎性反应-线粒体网络研究抑郁症肝郁脾虚病机及醒脾解郁方干预效应
Study on syndrome of stagnation of liver qi and spleen deficiency in depression based on corticosterone-inflammation-mitochondrial network and effects of Xingpi Jieyu Decoction

查看参考文献19篇

袁清洁 1   郭建友 2   王建伟 1   李阳 1   邓潇斐 2   郭蓉娟 3 *  
文摘 目的:从皮质酮-炎性反应-线粒体网络动态变化研究抑郁症肝郁脾虚病机及醒脾解郁方干预效应。方法:采用6周慢性不可预见性温和应激(CUMS)方法建立抑郁症肝郁脾虚大鼠模型,应激同时灌胃给药。于2、4、6周末采集各组血清,ELISA法检测皮质酮(CORT)、肿瘤坏死因子-α (TNF-α)、干扰素-γ(IFN-γ)、白介素-4 (IL-4)、白介素-6 (IL-6)及白介素-10 (IL-10)含量,于2、6周末行前额叶、肝脏取材,透射电镜观察线粒体形态。结果:应激2周时,CUMS大鼠出现CORT、TNF-α、IFN-γ、IL-6含量显著升高(P<0.01),IL-10含量显著降低(P<0.05),前额叶神经元、肝细胞线粒体轻微损伤;应激6周时,CUMS大鼠出现CORT、TNF-α、IFN-γ、IL-6含量显著升高(P<0.01),IL-4、 IL-10含量显著降低(P<0.01),前额叶神经元、肝细胞线粒体明显损伤。中药醒脾解郁方可早期拮抗CORT升高,抑制炎性反应及线粒体损伤。结论:应激早期可致CORT含量显著升高及炎性反应网络部分紊乱,应激后期皮质酮-炎性反应-线粒体网络明显失衡,表现为抑郁症肝郁脾虚证,中药保护作用明显。
其他语种文摘 Objective: To study the pathogenesis of stagnation of liver qi and spleen deficiency and the effect of Xingpi Jieyu Decoction on depression based on the changes in corticosterone-inflammation-mitochondrial network. Methods: A 6-week chronic unpredictable mild stress (CUMS) method was used to establish a rat model of depression which met the syndrome of stagnation of liver qi and spleen deficiency. The serum was collected at end of the 2nd,4th and 6th week, and the levels of CORT, TNF-α,IFN-γ,IL-4, IL-6 and IL-10 in serum were detected by Elisa. At 2 and 6 weeks, the prefrontal cortex and the liver tissue were harvested for the observation of the mitochondrial ultrastructure under transmission electron microscopy. Results: The levels of CORT,TNF-α,IFN-γ and IL-6 in serum were significantly increased (P<0.01) and IL-10 levels decreased (P<0.05) when rats were suffered from 2-week CUMS. Meanwhile, the mitochondria in the neuron of prefrontal cortex and the liver cell were slightly damaged. The levels of CORT, TNF-α,IFN--γ and IL-6 in serum were significantly increased (P<0.01) and the levels of IL-4 and IL-10 were significantly decreased (P<0.01) when rats were suffered from 6-week CUMS. Meanwhile, the mitochondria in the neuron of the prefrontal cortex and the liver cell were obviously damaged. Xingpi Jieyu Decoction early antagonized the increase of corticosterone and inhibited inflammation and mitochondrial damage. Conclusion: The CORT content is increased obviously and the inflammatory network is partially disturbed at the early stage of stress. The corticosterone-inflammation-mitochondrial network was imbalanced obviously at the late stage of stress, which manifested the syndrome of stagnation of liver qi and spleen deficiency. The Chinese medicine compound formula Xingpi Jieyu Decoction played obvious protective effect on the stressed rats.
来源 中华中医药杂志 ,2017,32(5):2241-2245 【核心库】
关键词 皮质酮 ; 炎性反应 ; 线粒体 ; 抑郁症 ; 肝郁脾虚 ; 肝气郁结 ; 肝脾相关
地址

1. 北京中医药大学, 北京, 100029  

2. 中国科学院心理研究所, 中国科学院心理健康重点实验室, 北京, 100101  

3. 北京中医药大学东方医院, 北京, 100078

语种 中文
文献类型 研究性论文
ISSN 1673-1727
学科 中国医学
基金 国家自然科学基金项目
文献收藏号 CSCD:5980332

参考文献 共 19 共1页

1.  Ledford Heidi. Medical research:lf depression were cancer. Nature,2014,515:182-184 CSCD被引 23 次    
2.  Kulkarni S K. Current investigational drugs for major depression. Expert Opin Investig Drugs,2009,18(6):767-788 CSCD被引 7 次    
3.  郭静. “郁病”从中焦论治. 中国中医基础医学杂志,2007,13(9):686-687 CSCD被引 3 次    
4.  姚玉璞. 从抑郁症的主要证型探讨“见肝之病,知肝传脾”. 中华中医药杂志,2013,28(4):1081-1083 CSCD被引 2 次    
5.  李玉波. 抑郁症肝郁脾虚证大鼠中枢及外周5-羟色胺的变化规律研究. 中华中医药杂志,2013,28(5):1267-1271 CSCD被引 6 次    
6.  王椿野. 醒脾解郁方对抑郁模型大鼠抑郁行为和血清5-羟色胺、皮质酮的影响. 世界中医药,2014(12):1633-1635,1639 CSCD被引 2 次    
7.  王椿野. 醒脾解郁方对抑郁模型大鼠学习记忆行为及炎性因子水平的影响. 北京中医药,2014(7):503-506 CSCD被引 2 次    
8.  Stetler C. Depression and hypothalamic-pituitary-adrenal activation:a quantitative summary of four decades of research. Psychosom Med,2011,73(2):114-126 CSCD被引 32 次    
9.  Bhagwagar Z. Increase in concentration of waking salivary corticosterone in recovered patients with depression. Am J Psychiatry,2003,160(10):1890-1891 CSCD被引 4 次    
10.  Holsen L M. HPA-axis hormone modulation of stress response circuitry activity in women with remitted major depression. Neuroscience,2013,250:733-742 CSCD被引 4 次    
11.  Maes M. Evidence for an immune response in major depression:a review and hypothesis. Prog Neuro-Psychopharmacol Biol Psychiat,1995,19(1):11-38 CSCD被引 27 次    
12.  Lichtblau N. Cytokines as biomarkers in depressive disorden:current standing and prospects. Int Rev Psychiat,2013,25(5):592-603 CSCD被引 12 次    
13.  Patel A. Review:the role of inflammation in depression. Psychiatria Danubina,2013,25(2):216-223 CSCD被引 4 次    
14.  林文娟. 抑郁症的心理神经免疫学研究：细胞因子的作用. 心理科学进展,2008,16(3):404-410 CSCD被引 18 次    
15.  徐说. 抗炎性细胞因子与抑郁症. 生物化学与生物物理进展,2014,41(11):1099-1108 CSCD被引 9 次    
16.  朱颖. 血清IL-6、IL-10及IFN-α水平对首发抑郁患者双侧海马区细胞代谢影响的磁共振波谱研究. 中风与神经疾病杂志,2013,30(10):891-894 CSCD被引 2 次    
17.  臧雨馨. 慢性不可预见性温和应激模型大鼠的肝脏药物代谢酶活性变化研究. 药学学报,2015,50(3):319-325 CSCD被引 3 次    
18.  徐舒. "肝郁证"大鼠模型的建立及代谢组学的初步研究. 中华中医药杂志,2009,24(6):787-791 CSCD被引 22 次    
19.  丁霞. 论脾虚及能量代谢障碍是抑郁症发病的关键病机. 中医杂志,2016,57(11):924-926 CSCD被引 12 次    
引证文献 15 篇

1 姜默琳 近5年中药复方抗抑郁机制研究进展 中华中医药杂志,2018,33(10):4571-4574
CSCD被引 3 次

2 于姚 抑郁症的线粒体能量代谢障碍刍议 北京中医药大学学报,2019,42(7):602-606
CSCD被引 4 次

显示所有15篇文献

论文科学数据集
PlumX Metrics
相关文献

 作者相关
 关键词相关
 参考文献相关

版权所有 ©2008 中国科学院文献情报中心 制作维护：中国科学院文献情报中心
地址：北京中关村北四环西路33号 邮政编码：100190 联系电话：(010)82627496 E-mail:cscd@mail.las.ac.cn 京ICP备05002861号-4 | 京公网安备11010802043238号