山东地区海水养殖区常见抗生素耐药菌及耐药基因分布特征
Distribution characteristics of antibiotic resistance bacteria and related resistance genes in mariculture area of Shandong
查看参考文献34篇
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文摘
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对山东地区海水养殖海域常见抗生素耐药菌及耐药基因分布进行调查,结果表明所调查5个海水养殖区水样中四环素类、磺胺类、β-内酰胺类耐药菌比例显著(p<0.05)高于氯霉素类和喹诺酮类耐药菌;此外用RT-PCR方法对共计15种耐药基因在水样中的丰度测定表明磺胺类(sul、dfra/16S rRNA= 10~(-6) ~10~(-2))、喹诺酮类(qnr/16S rRNA=10~(-6)~ 10~(-2))以及四环素类耐药基因(tet/16S rRNA= 10~(-7) ~10~(-2))在各水样中丰度差异不显著,而氯霉素耐药基因(cata、cmle/16S rRNA=10~(-8)~10~(-2))在不同水样中丰度差异显著,且catal和cmlel丰度与可培养氯霉素耐药菌比例存在相关线性关系。实验数据说明山东海水养殖区水样中存在一定的抗生素耐药菌和耐药基因污染。 |
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其他语种文摘
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Antibiotic resistance bacteria and related resistance genes in five mariculture areas of Shandong were detected,and in both five samples it was found that the percentage of tetracycline-,Sulfonamides- and β-lactam-resistant bacteria were significantly (p < 0.05) higher than those chloromycetin-and quinolone-resistant bacteria. The abundance of 15 antimicrobial resistant genes was determined by RT-PCR methods. It was obtained that the abundance of sulfonamides- (sul,dfra/16S rRNA= 10~(-6)~ 10~(-2)), quinolone-(qnr/16S rRNA= 10~(-6) ~10~(-2)),and tetracycline-resistance genes (tet/16S rRNA= 10 ~(-7) ~ 10~(-2)) has no significant differences among the five water samples,but the abundance of chloramphenicol - resistant genes (cata, cmle/16S rRNA= 10~(-8) ~ 10~(-2)) has significant differences among the five water samples and the abundance of the cata1 and cmle1 has significant correction (p <0.05) with the percentage of chloramphenicol-resistant bacteria. All the results indicated the emergence pollution of antimicrobial resistant bacteria and related resistance genes in mariculture environments of Shandong, China. |
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来源
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海洋环境科学
,2016,35(1):55-62 【核心库】
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关键词
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海水养殖区
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耐药菌
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耐药基因
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RT-PCR定量
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地址
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中国海洋大学,生物多样性与进化研究所, 山东, 青岛, 266003
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国家海洋局第一海洋研究所, 山东, 青岛, 266061
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1007-6336 |
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学科
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环境污染及其防治 |
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基金
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国家海洋公益性行业科研专项
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国家科技支撑计划项目
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文献收藏号
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CSCD:5633340
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