Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis
查看参考文献43篇
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文摘
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The cottonmealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution. |
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来源
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Insect Science
,2015,22(6):761-767 【核心库】
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DOI
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10.1111/1744-7917.12181
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关键词
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expressed sequence tags
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microsatellite marker
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normalized cDNA library
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Phenacoccus solenopsis
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地址
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1.
College of Agronomy and Plant Protection, Qingdao Agricultural University, Key Lab of Integrated Crop Pest Management of Shandong Province, Shandong, Qingdao
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Shenyang Entry-Exit Inspection and Quarantine Bureau, Shenyang
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College of Agronomy and Plant Protection, Qingdao Agricultural University, Key Lab of Integrated Crop Pest Management of Shandong Province;;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Shandong, Qingdao
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1672-9609 |
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学科
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遗传学 |
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基金
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the Taishan Mountain Scholar Constructive Engineering Foundation of Shandong, and the Shandong Modern Agricultural Technology & Industry System
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the Science and Technology Development Planning Program of Qingdao
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supported by the National Science and Technology Support Program
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文献收藏号
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CSCD:5582479
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