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Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BK_(Ca) channels and reducing intracellular Ca~(2+) levels

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文摘 Aim: Sustained pulmonary vasoconstriction as experienced at high altitude can lead to pulmonary hypertension (PH). The main purpose of this study is to investigate the vasorelaxant effect of echinacoside (ECH), a phenylethanoid glycoside from the Tibetan herb Lagotis brevituba Maxim and Cistanche tubulosa, on the pulmonary artery and its potential mechanism. Methods: Pulmonary arterial rings obtained from male Wistar rats were suspended in organ chambers filled with Krebs-Henseleit solution, and isometric tension was measured using a force transducer. Intracellular Ca~(2+) levels were measured in cultured rat pulmonary arterial smooth muscle cells (PASMCs) using Fluo 4-AM. Results: ECH (30-300 μmol/L) relaxed rat pulmonary arteries precontracted by noradrenaline (NE) in a concentration-dependent manner, and this effect could be observed in both intact endothelium and endothelium-denuded rings, but with a significantly lower maximum response and a higher EC_(50) in endothelium-denuded rings. This effect was significantly blocked by L-NAME, TEA, and BaCl_2 However, IMT, 4-AP, and Gli did not inhibit ECH-induced relaxation. Under extracellular Ca~(2+)-free conditions, the maximum contraction was reduced to 24.54%±2.97% and 10.60%±2.07% in rings treated with 100 and 300 μmol/L of ECH, respectively. Under extracellular calcium influx conditions, the maximum contraction was reduced to 112.42%±7.30%, 100.29%±8.66%, and 74.74%±4.95% in rings treated with 30, 100, and 300 μmol/L of ECH, respectively. After cells were loaded with Fluo 4-AM, the mean fluorescence intensity was lower in cells treated with ECH (100 μmol/L) than with NE. Conclusion: ECH suppresses NE-induced contraction of rat pulmonary artery via reducing intracellular Ca~(2+) levels, and induces its relaxation through the NO-cGMP pathway and opening of K~+ channels (BK_(Ca) and K_(IR)).
来源 Acta Pharmacologica Sinica ,2015,36(5):587-596 【核心库】
DOI 10.1038/aps.2014.126
关键词 Tibetan herb ; echinacoside ; pulmonary hypertension ; high altitude ; vasorelaxation ; NO ; BK_(Ca) ; K_(IR) ; artery ring ; pulmonary arterial smooth muscle cells
地址

1. Department of Pharmacology, School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016  

2. Qinghai Entry-Exit Inspection and Quarantine Bureau, Xining, 810001  

3. Research Center for High Altitude Medicine, Medical College of Qinghai University, Xining, 810001

语种 英文
文献类型 研究性论文
ISSN 1671-4083
学科 药学
基金 supported by the National Program on Key Basic Research Project of China ;  国家自然科学基金 ;  the Program of International Science and Technology Cooperation of China ;  国家自然科学基金 ;  the Key Laboratories Development Program of Qinghai Province ;  Qinghai-Utah Joint Research Key Lab for High Altitude Medicine ;  the Ministry of Education New Century Outstanding Talents Support Program of China ;  the Natural Science Foundation of Qinghai
文献收藏号 CSCD:5425432

参考文献 共 29 共2页

1.  Hultgren H N. High Altitude Medicine,1997 CSCD被引 1 次    
2.  Humbert M. Treatment of pulmonary arterial hypertension. N Engl J Med,2004,351:1425-1436 CSCD被引 38 次    
3.  Ge R L. Life on the Qinghai-Tibetan Plateaus. Chinese,2007 CSCD被引 1 次    
4.  Xin Y. Determination of echinacoside and acteoside in Tibetan herb Lagotis brevituba Maxim. Chin J Pharm Anal,2012,32:1183-1185 CSCD被引 1 次    
5.  Zhao Q. Neurotrophic and neurorescue effects of Echinacoside in the subacute MPTP mouse model of Parkinson's disease. Brain Res,2010,1346:224-236 CSCD被引 10 次    
6.  Yang X L. Efficacy and safety of echinacoside in a rat osteopenia model. Evid Based Complement Alternat Med,2013,2013:1-10 CSCD被引 1 次    
7.  He W J. Echinacoside elicits endothelium-dependent relaxation in rat aortic rings via an NO-cGMP pathway. Planta Med,2009,75:1400-1404 CSCD被引 2 次    
8.  Warshaw D M. Mechanical and morphological properties of arterial resistance vessels in young and old spontaneously hypertensive rats. Circ Res,1979,45:250-259 CSCD被引 1 次    
9.  Shen M. The vasorelaxing effect of resveratrol on abdominal aorta from rats and its underlying mechanisms. Vascul Pharmacol,2012,58:64-70 CSCD被引 3 次    
10.  Gai X Y. Antiproliferative effect of echinacoside on rat pulmonary artery smooth muscle cells under hypoxia. J Pharmacol Sci,2014,126:155-163 CSCD被引 3 次    
11.  Mandal S K. Tissue factor trafficking in fibroblasts: involvement of protease-activated receptor-mediated cell signaling. Blood,2007,110:161-170 CSCD被引 3 次    
12.  Toque H A. Vardenafil, but not sildenafil or tadalafil, has calcium-channel blocking activity in rabbit isolated pulmonary artery and human washed platelets. Br J Pharmacol,2008,154:787-796 CSCD被引 1 次    
13.  Fonseca-Magalhaes P A. Inhibitory effects of sertraline in rat isolated perfused kidneys and in isolated ring preparations of rat arteries. J Pharm Pharmacol,2011,63:1186-1194 CSCD被引 1 次    
14.  Berridge M J. Inositol trisphosphate and calcium signalling mechanisms. Biochim Biophys Acta,2009,1793:933-940 CSCD被引 26 次    
15.  Hu F. Involvements of calcium channel and potassium channel in Danshen and Gegen decoction induced vasodilation in porcine coronary LAD artery. Phytomedicine,2012,19:1051-1058 CSCD被引 3 次    
16.  Matsumoto T. Vasodilator effect of Cassiarin A, a novel antiplasmodial alkaloid from Cassia siamea, in rat isolated mesenteric artery. Biol Pharm Bull,2010,33:844-848 CSCD被引 2 次    
17.  Dubuis E. Continuous inhalation of carbon monoxide attenuates hypoxic pulmonary hypertension development presumably through activation of BK_(Ca) channels. Cardiovasc Res,2005,65:751-761 CSCD被引 5 次    
18.  Moudgil R. The role of K~+ channels in determining pulmonary vascular tone, oxygen sensing, cell proliferation, and apoptosis: implications in hypoxic pulmonary vasoconstriction and pulmonary arterial hypertension. Microcirculation,2006,13:615-632 CSCD被引 17 次    
19.  Eichhorn B. Vascular large conductance calcium-activated potassium channels: functional role and therapeutic potential. Naunyn Schmiedebergs Arch Pharmacol,2007,376:145-155 CSCD被引 8 次    
20.  Ko E A. Physiological roles of K~+ channels in vascular smooth muscle cells. J Smooth Muscle Res,2008,44:65-81 CSCD被引 29 次    
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