纯钛表面涂层(Ca+Zn)/P 比值与成骨细胞生物活性的关系
Study of relationship between (Ca+Zn)/P of pure titanium surface coating and biological activity of osteoblasts
查看参考文献16篇
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文摘
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目的:研究纯钛表面不同(Ca+Zn)/P 比值对成骨细胞(MC3T3-E1)黏附、增殖和分化的影响,确定纯钛表面生物活性最佳的 Ca、P、Zn 含量。方法:在微弧氧化的电解液中加入一定浓度的 Ca、P 和5种不同浓度的 Zn(0、0.01、0.03、0.04和0.06 mol/L),使 Ca、P 元素的摩尔比值接近羟基磷灰石的比值。在纯钛材料表面制备5种不同(Ca+Zn)/P 比值的生物活性涂层,分别记为 S_0、S_1、S_2、S_3和 S_4组。应用 XPS 和 SEM 分析样品表面的元素组成、存在形式、(Ca+Zn)/P 比值以及表面形貌;应用 SEM、MTT 法和 ALP 活性测定法分析材料表面培养的MC3T3-E1细胞黏附、增殖和分化情况。采用 SPSS13.0软件包对数据进行统计学分析。结果:随着电解液中 Zn 浓度的增加,S_0~S_4组(Ca+Zn)/P 比值分别为2.94、2.64、2.71、2.87和2.72,其中,在 S_3组时达到最大,S_4组时降低。MC3T3-E1细胞在5组材料表面黏附、增殖和分化能力,依次为 S_3>S_4>S_2>S_1>S_0,各组之间差异具有显著性(P<0.05)。结论:应用微弧氧化法在纯钛表面制备的不同含量Ca、P、Zn 涂层,随着(Ca+Zn)/P比值的增大,成骨细胞的黏附、增殖和分化能力随之增强。其中,(Ca+Zn)/P比值为2.87时,细胞的生物活性最佳。 |
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其他语种文摘
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PURPOSE: To study osteoblast (MC3T3-E1 cells) adhesion, proliferation and differentiation activity on the surface of pure titanium with different (Ca+Zn)/P ratios,and determine Ca,P,Zn contents offering best biological activity on pure titanium surface. METHODS: In the micro-arc oxidation electrolyte, Ca, P with a certain concentration, and 5 different concentrations of Zn (0,0.01,0.03,0.04, and 0.06 mol/L) were added, which made the mole ratio of Ca and P element close to the ratio of hydroxyapatite. (Ca +Zn)/P ratio of bioactive coating on 5 different pure titanium material surfaces were prepared, which named S_0 and S_1, S_2, S_3 and S_4 group, respectively. The surface elements, existence form, (Ca + Zn)/P ratio and surface morphology were analyzed by XPS and SEM; MC3T3 -E1 cells adhesion, proliferation and differentiation cultured on the material surface were analyzed by SEM, MTT method and ALP activity assay. RESULTS: The data was analyzed with SPSS13.0 software package. With the increase of Zn content in the electrolyte, (Ca+Zn)/P ratios were 2.94, 2.64 2.71,2.87 and 2.72 in S_0 ~ S_4 group, respectively. (Ca+Zn)/P ratio was maximal in S 3 group and decreased in S_4 group. MC3T3-E1 cell adhesion, proliferation and differentiation capacity on material surface of 5 groups changed in the following order: S_3 > S_4 > S_2 > S_1 > S_0, with significant differences among 5 groups (P <0.05). CONCLUSIONS: Coating with different concentrations of Ca, P, Zn on pure titanium surface by micro-arc oxidation technique, the adhesion, proliferation and differentiation ability of osteoblast increase as (Ca+Zn)/P ratio increase, and cell biological activity is the best when the (Ca+Zn)/P ratio reached 2.87. Supported by Natural Science Foundation of Liaoning Province (20092093), Science and Technology Project of Liaoning Province (2013225049), and Science and Technology Project of Shenyang City (F11-264-1-25). |
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来源
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上海口腔医学
,2015,24(1):46-51 【扩展库】
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关键词
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微弧氧化
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钛
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锌
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钙
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磷
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成骨细胞
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地址
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中国医科大学附属口腔医院种植中心,辽宁省口腔医学研究所口腔种植研究室,辽宁省口腔疾病转化医学研究中心, 辽宁, 沈阳, 110002
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中国科学院金属腐蚀与防护研究所, 辽宁, 沈阳, 110016
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中国医科大学口腔医学院中心实验室, 辽宁, 沈阳, 110002
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1006-7248 |
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学科
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口腔科学 |
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基金
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辽宁省自然科学基金
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辽宁省科技计划项目
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辽宁省沈阳市科技计划项目
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文献收藏号
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CSCD:5357635
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