外源性音猥因子对成年大鼠脊髓损伤后室管膜区神经干细胞增殖的影响
Effects of exogenous sonic hedgehog on proliferation of neural stem cells in ependymal area after spinal cord injury in adult rats
查看参考文献21篇
|
文摘
|
目的研究外源性音猥因子(sonic hedgehog, Shh)对成年大鼠脊髓损伤(spinal cord injury, SCI)后室管膜区神经干细胞(neural stem cells, NSCs)的增殖及运动功能恢复的影响。方法雌性SD大鼠55只,任意选取5只作为正常对照组,余50只采用改良Allen打击法于T10节段制作大鼠急性SCI模型,按随机数字表法分为Shh组和SCI组,每组25只。分别于伤后1,3,7,14,28 d采用改良Tarlov评分评价大鼠后肢运动功能恢复程度;免疫荧光双标染色观察大鼠SCI后Brdu和Nestin双阳性细胞数量的变化情况。结果术后7 d起,Shh组和SCI组Tarlov评分比较差异有统计学意义(P<0.05)。免疫荧光双标染色显示,Shh组自术后3 d起各时相点Brdu/Nestin 双阳性细胞数量分别为97. 20 ± 18. 23,153. 60 ± 25. 76,133. 80 ± 21. 02,89. 80 ± 15. 42,较SCI组72.60±15.60,112.20 ±23.63, 94.20±18.70, 43.40±10.62明显增多(P均 <0. 05) 。 结论外源性Shh可促进成体大鼠SCI伤后室管膜区NSCs增殖以及运动功能的恢复。 |
|
其他语种文摘
|
Objective To investigate effects of exogenous sonic hedgehog (Shh) on proliferation of neural stem cells (NSCs) in ependymal area and recovery of motor function after spinal cord injury (SCI) in adult rats. Methods Fifty-five female SD rats were involved in the study : five were selected as normal control group and fifty as Shh group (n = 25) and SCI group (n = 25) after being subjected to SCI at T10 segment using the modified Allen ' s method according to the random number table. At 1,3,7, 14,and 28 days after operation, restoration of hindlimb motor function of SD rats was assessed with modified Tarlov scale and changes of double positive cells of Brdu and Nestin with double-stained immunofluorescence. Results Tarlov scale revealed statistical difference between Shh and SCI groups since days 7 postoperatively (P <0. 05). In the double-staining test, number of double positive cells of Brdu and Nestin was greater in Shh Group than in SCI Group since day 3 postoperatively [ (97. 20 ± 18. 23) vs (72.60±15.60), (153.60 ±25.76) vs (112.20 ±23.63), (133. 80 ±21.02) vs (94.20 ± 18.70),(89.80 ±15.42) vs (43.40 ± 10. 62),P<0.05]. Conclusion Exogenous Shh is conducive to the proliferation of ependymal NSCs and the recovery of motor function in SCI rats. |
|
来源
|
中华创伤杂志
,2014,30(7):740-743 【核心库】
|
|
关键词
|
脊髓损伤
;
干细胞
;
音猥因子
|
|
地址
|
兰州大学第二医院骨科, 甘肃省骨与关节疾病重点实验室, 兰州, 730030
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1001-8050 |
|
学科
|
医药、卫生;基础医学 |
|
基金
|
甘肃省自然科学基金
|
|
文献收藏号
|
CSCD:5207807
|
参考文献 共
21
共2页
|
|
1.
Bambakidis N C. Endogenous stem cell proliferation after central nervous system injury: alternative therapeutic options.
Neurosurg Focus,2005,19(3):E1
|
CSCD被引
4
次
|
|
|
|
|
2.
Briscoe J. Homeobox gene Nkx-2 and specification of neuronal identity by graded sonic hedgehog signalling.
Nature,1999,398(6728):622-627
|
CSCD被引
8
次
|
|
|
|
|
3.
胡慧敏. 音猬因子体外诱导人骨髓基质干细胞转化为神经元样细胞的研究.
中国脊柱脊髓杂志,2006,16(5):380-383
|
CSCD被引
2
次
|
|
|
|
|
4.
Sloane-Stanley G H. Intrathecal injections in rats by percutaneous lumbar puncture.
J Pharm Pharmacol,1981,33(7):480-482
|
CSCD被引
4
次
|
|
|
|
|
5.
Gale K. Spinal cord contusion in the rat: behavioral analysis of functional neurologic impairment.
Exp Neurol,1985,88(1):123-134
|
CSCD被引
43
次
|
|
|
|
|
6.
Dorota C. Sonic hedgehog signaling in the developing and adult brain.
J Physiol,2002,96(1):9-16
|
CSCD被引
2
次
|
|
|
|
|
7.
Britto J. A critical role for sonic hedgehog signaling in the early expansion of the developing brain.
Nat Neurosci,2002,5(2):103-110
|
CSCD被引
5
次
|
|
|
|
|
8.
许汉鹏. 音猬因子的功能受体斑片在培养神经干细胞中的表达.
解剖学报,2002,33(6):561-564
|
CSCD被引
6
次
|
|
|
|
|
9.
侯天勇. 转录调控因子Shh促进胎鼠脊髓神经干细胞体外增殖的效应.
中国临床康复,2004,8(32):7162-7163,7241
|
CSCD被引
5
次
|
|
|
|
|
10.
曲静. SHH方案诱导胚胎干细胞分化为神经干细胞移植促进小鼠损伤脊髓结构与功能的恢复.
中华神经外科杂志,2004,20(3):196-201
|
CSCD被引
2
次
|
|
|
|
|
11.
Ciaroni S. Impairment of neural precursor proliferation increases survival of cell progeny in the adult rat dentate gyrus.
Mech Ageing Dev,2002,123(10):1341-1352
|
CSCD被引
11
次
|
|
|
|
|
12.
Lendahl U. CNS stem cells express a new class of intermediate filament protein.
Cell,1990,60(4):585-595
|
CSCD被引
228
次
|
|
|
|
|
13.
Bambakidis N C. Transplantation of oligodendrocyte precursors and sonic hedgehog results in improved function and white matter sparing in the spinal cords of adult rats after contusion.
Spine J,2004,4(1):16-26
|
CSCD被引
15
次
|
|
|
|
|
14.
Tsuboi K. Intrastriatal injection of sonic hedgehog reduces behavioral impairment in a rat model of Parkinson's disease.
Exp Neurol,2002,173(1):95-104
|
CSCD被引
1
次
|
|
|
|
|
15.
Bambakidis N C. Improvement of neurological recovery and stimulation of neural progenitor cell proliferation by intrathecal administration of Sonic hedgehog.
J Neurosurg,2012,116(5):1114-1120
|
CSCD被引
10
次
|
|
|
|
|
16.
戎祯祥. Sonic hedgehog信号通路Smo蛋白及其下游转录因子Glil蛋白在胃癌组织中的表达及其意义.
南方医科大学学报,2006,26(12):1728-1731
|
CSCD被引
8
次
|
|
|
|
|
17.
Lai K. Sonic hedgehog regulates adult neural progenitor proliferation in vitro and in vivo.
Nat Neurosci,2003,6(1):21-27
|
CSCD被引
38
次
|
|
|
|
|
18.
Ruizi Altaba A. Hedgehog-Gli signaling and the growth of the brain.
Nat Rev Neurosci,2002,3(1):24-33
|
CSCD被引
12
次
|
|
|
|
|
19.
Xie J. A role of PDGFRalpha in basal cell carcinoma proliferation.
Proc Natl Acad Sci USA,2001,98(16):9255-9259
|
CSCD被引
6
次
|
|
|
|
|
20.
Hahn H. Patched target Igf2 is indispensable for the formation of medulloblastoma and rhabdomyosarcoma.
J Biol Chem,2000,275(37):28341-28344
|
CSCD被引
2
次
|
|
|
|
|
了解气象卫星更多信息，请访问