Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchymal stem cells
查看参考文献40篇
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文摘
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microRNAs (miRNAs) play an important regulatory role in the self-renewal and differentiation of stem cells. In this study, we examined the effects of miRNA-124 (miR-124) overexpression in bone marrow-derived mesenchymal stem cells. In particular, we focused on the effect of overexpression on the differentiation of bone marrow-derived mesenchymal stem cells into neurons. First, we used GeneChip technology to analyze the expression of miRNAs inbone marrow-derived mesenchymal stem cells, neural stem cells and neurons. miR-124 expression was substantially reduced inbone marrow-derived mesenchymal stem cells compared with the other cell types. We constructed a lentiviral vector overexpressing miR-124 and transfected it intobone marrow-derived mesenchymal stem cells. Intracellular expression levels of the neuronal early markersβ-III tubulin and microtubule-associated protein-2 were signiifcantly increased, and apoptosis induced by oxygen and glucose deprivation was reduced in transfected cells. After miR-124-transfected bone marrow-derived mesenchymal stem cells were transplanted into the injured rat spinal cord, a large number of cells positive for the neuronal marker neurofilament-200 were observed in the transplanted region. The Basso-Beattie-Bresnahan locomotion scores showed that the motor function of the hind limb of rats with spinal cord injury was substantially improved. These results suggest that miR-124 plays an important role in the differentiation ofbone marrow-derived mesenchymal stem cells into neurons. Our ifndings should facilitate the development of novel strategies for enhancing the therapeutic efifcacy ofbone marrow-derived mesenchymal stem cell transplantation for spinal cord injury. |
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来源
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Neural Regeneration Research
,2014,9(12):1241-1248 【核心库】
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DOI
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10.4103/1673-5374.135333
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关键词
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nerve regeneration
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microRNA-124
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lentivirus
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overexpression
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bone marrow-derived mesenchymal stem cells
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neural stem cells
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spinal cord injury
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neurogenesis
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GeneChip
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motor function
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NSFC grant
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neural regeneration
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地址
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1.
Department of Orthopedics, First Affiliated Hospital of China Medical University, Liaoning, Shenyang
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Department of Orthopedics, Jinhua Central Hospital of Zhejiang University, Zhejiang, Jinhua
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1673-5374 |
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学科
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基础医学;药学 |
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基金
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国家自然科学基金
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文献收藏号
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CSCD:5185878
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