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An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants

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文摘 Abscisic acid (ABA) is the most important hormone for plants to resist drought and other abiotic stresses. ABA binds directly to the PYR/PYL family of ABA receptors, resulting in inhibition of type 2C phosphatases (PP2C) and activation of downstream ABA signaling. It is envisioned that intervention of ABA signaling by small molecules could help plants to overcome abiotic stresses such as drought, cold and soil salinity. However, chemical instability and rapid catabolism by plant enzymes limit the practical application of ABA itself. Here we report the identification of a small molecule ABA mimic (AM1) that acts as a potent activator of multiple members of the family of ABA receptors. In Arabidopsis, AM1 activates a gene network that is highly similar to that induced by ABA. Treatments with AM1 inhibit seed germination, prevent leaf water loss, and promote drought resistance. We solved the crystal structure of AM1 in complex with the PYL2 ABA receptor and the HAB1 PP2C, which revealed that AM1 mediates a gate-latch-lock interacting network, a structural feature that is conserved in the ABA-bound receptor/PP2C complex. Together, these results demonstrate that a single small molecule ABA mimic can activate multiple ABA receptors and protect plants from water loss and drought stress. Moreover, the AM1 complex crystal structure provides a structural basis for designing the next generation of ABA-mimicking small molecules.
来源 Cell Research ,2013,23(8):1043-1054 【核心库】
DOI 10.1038/cr.2013.95
关键词 abscisic acid ; plant hormone ; drought resistance ; crystal structure ; ABA-mimicking ligand
地址

1. Shanghai Center for Plant Stress Biology and Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032  

2. Shanghai Center for Plant Stress Biology and Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Key Laboratory of Receptor Research, Chinese Academy of Sciences, Shanghai, 200032  

3. Institute of Chemical Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangdong, Guangzhou, 510530  

4. Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute, USA, Grand Rapids, 49503  

5. Department of Horticulture and Landscape Architecture, Purdue University, USA, West Lafayette, 47906  

6. Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032  

7. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203  

8. VARI-SIMM Center, Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Key Laboratory of Receptor Research, Chinese Academy of Sciences, Shanghai, 201203

语种 英文
文献类型 研究性论文
ISSN 1001-0602
学科 细胞生物学
基金 supported by the Jay and Betty Van Andel Foundation ;  Amway (China) ;  国家自然科学基金 ;  US National Institute of Health ;  Funding for the Shanghai Center for Plant Stress Biology supported by Chinese Academy of Sciences ;  the 100-talent program of Chinese Academy of Sciences
文献收藏号 CSCD:4907353

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引证文献 21 篇

1 刘声传 茶树耐旱机理及抗旱节水研究进展 茶叶科学,2014,34(2):111-121
CSCD被引 7 次

2 Ng Ley Moy Abscisic acid perception and signaling: structural mechanisms and applications Acta Pharmacologica Sinica,2014,35(5):567-584
CSCD被引 0 次

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