利用反硝化细菌法测试水体硝酸盐氮氧同位素
Measurement of waters nitrate dual isotopes based on denitrifier method
查看参考文献22篇
|
文摘
|
反硝化细菌方法作为测试硝酸盐氮氧同位素组成的最新方法,具有可测试低浓度水样、对样品无需特殊处理、不会交叉污染和需样量少等诸多优点而得到迅速发展。本研究率先在国内实验室利用反硝化细菌法成功测试了硝酸盐氮氧同位素组成,将经过5~10d培养的反硝化细菌Pseudomonas aureofaciens离心,然后将菌液浓缩5倍,再向顶空进样瓶注入3mL菌液,密封后利用高纯氮气吹扫3h以上,注入50nmolNO_3-水样经过夜培养灭活后,使用TraceGasPre-concentrator-Isoprime测试N_2O同位素组成,结果表明,重现性和测试精度与国际上类似研究接近。该方法的建立对于国内开展河流及湖泊(水库)、降水等氮的生物地球化学循环将起到促进作用。 |
|
其他语种文摘
|
Denitrifier method is a novel method for nitrogen and oxygen isotopic analysis of nitrate, which has many advantages, e.g., low nitrate water sample, simple pretreatment, no cross-contamination, and small volume water, and obtained rapid popularization. In this study, the method was first developed in China for nitrate isotopic measurement in our laboratory. The denitrifier (Pseudomonas aureofaciens) was grown in an amended medium of Tryptic Soy Broth. After 5-10 days culture, the cells were harvested and concentrated 5 folds, and split into 3 mL aliquots in 20 mL headspace vials. The vials were sealed and then purged for at least 3 h with high-purity N_2 to ensure anaerobic condition. A sample amount corresponding to 50 nmol of nitrate was added to the sample vials, and cultured overnight to allow the complete conversion of NO_3~- to N_2O. On the following day, 0.1-0.2 mL of 10 mol·LC~(-1) NaOH was injected into the vials to lyse the bacterium and to scrub any CO_2 gas. Simultaneous δ~(15)N and δ~(18)O analysis of the produced N_2O was carried out by using a Trace Gas Pre-concentrator unit coupled to an IRMS instrument. The measured data showed good reproducibility and precision, in close with the similar international studies. The establishment of this method would promote our studies in nitrogen biogeochemical cycle in rivers and lakes (reservoirs) and in precipitation. |
|
来源
|
生态学杂志
,2012,31(8):2152-2157 【核心库】
|
|
关键词
|
反硝化细菌法
;
硝酸盐
;
氮同位素
;
氧同位素
|
|
地址
|
中国科学院地球化学研究所, 环境地球化学国家重点实验室, 贵阳, 550002
|
|
语种
|
中文 |
|
ISSN
|
1000-4890 |
|
学科
|
环境科学基础理论 |
|
基金
|
中国科学院知识创新工程重要方向项目
;
国家自然科学基金项目
|
|
文献收藏号
|
CSCD:4600522
|
参考文献 共
22
共2页
|
|
1.
刘丛强.
生物地球化学过程与地表物质循环———西南喀斯特流域侵蚀与生源要素循环,2007
|
CSCD被引
3
次
|
|
|
|
|
2.
毛绪美. 细菌反硝化法同时分析天然水中硝酸盐氮、氧同位素组成研究.
地质科技情报,2006,25(5):97-100
|
CSCD被引
11
次
|
|
|
|
|
3.
张翠云. 硝酸盐氮同位素反硝化细菌法测试技术的建立.
核技术,2011,34(3):237-240
|
CSCD被引
7
次
|
|
|
|
|
4.
岳甫均. 利用氮同位素技术探讨天津地表水氮污染.
生态学杂志,2010,29(7):1403-1408
|
CSCD被引
20
次
|
|
|
|
|
5.
Beremner J M. Determination and isotoperatio analysis of different forms of nitrogen in soils. Ⅰ. Apparatus and procedure for distillation and determination of ammonium.
Soil Science Society of America Proceedings,1965:504-507
|
CSCD被引
1
次
|
|
|
|
|
6.
Bohlke J K. Oxygen isotopes in nitrate: New reference materials for (18)O: (17)O: (16)O measurements and observations on nitrate-water equilibration.
Rapid Communications in Mass Spectrometry,2003,17:1835-1846
|
CSCD被引
12
次
|
|
|
|
|
7.
Casciotti K L. Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method.
Analytical Chemistry,2002,74:4905-4912
|
CSCD被引
55
次
|
|
|
|
|
8.
Coplen T B. Using dual-bacterial denitrification to improve δ15N determinations of nitrates containing mass-independent 17O.
Rapid Communications in Mass Spectrometry,2004,18:245-250
|
CSCD被引
3
次
|
|
|
|
|
9.
Fang Y T. Anthropogenic imprints on nitrogen and oxygen isotopic composition of precipitation nitrate in a nitrogen-polluted city in southern China.
Atmospheric Chemistry and Physics,2011,11:1313-1325
|
CSCD被引
12
次
|
|
|
|
|
10.
Hastings M G. Isotopic evidence for source changes of nitrate in rain at Bermuda.
Journal of Geophysical Research,2003:108
|
CSCD被引
12
次
|
|
|
|
|
11.
Hastings M G. Seasonal variations in N and O isotopes of nitrate in snow at Summit, Greenland: Implications for the study of nitrate in snow and ice cores.
Journal of Geophysical Research,2004,109:D20306
|
CSCD被引
4
次
|
|
|
|
|
12.
Kaiser J. Triple oxygen isotope analysis of nitrate using the denitrifier method and thermal decomposition of N2O.
Analytical Chemistry,2007,79:599-607
|
CSCD被引
9
次
|
|
|
|
|
13.
Kendall C. Tracing anthropogenic inputs of nitrogen to ecosystems.
Stable Isotopes in Ecology and Environmental Science(2nd ed.),2007
|
CSCD被引
1
次
|
|
|
|
|
14.
Li S L. Tracing the sources of nitrate in karstic groundwater in Zunyi, Southwest China: A combined nitrogen isotope and water chemistry approach.
Environmental Earth Sciences,2010,60:1415-1423
|
CSCD被引
7
次
|
|
|
|
|
15.
Li S L. Assessment of the sources of nitrate in the Changjiang River, China: Using a nitrogen and oxygen isotopic approach.
Environmental Science and Technology,2010,44:1573-1578
|
CSCD被引
65
次
|
|
|
|
|
16.
Liu C Q. Using ~(15)N and ~(18)O values to identify nitrate sources in karst ground water, Guiyang, Southwest China.
Environmental Science and Technology,2006,40:6928-6933
|
CSCD被引
85
次
|
|
|
|
|
17.
Mcllvin M R. Technical updates to the bacterial method for nitrate isotopic analyses.
Analytical Chemistry,2011,83:1850-1856
|
CSCD被引
12
次
|
|
|
|
|
18.
Sigman D M. Natural abundance-level measurement of the nitrogen isotopic composition of oceanic nitrate: An adaptation of the ammonia diffusion method.
Marine Chemistry,1997,57:227-242
|
CSCD被引
20
次
|
|
|
|
|
19.
Sigman D M. A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater.
Analytical Chemistry,2001,73:4145-4153
|
CSCD被引
72
次
|
|
|
|
|
20.
Silva S R. A new method for collection of nitrate from fresh water and the analysis of nitrogen and oxygen isotope ratios.
Journal of Hydrology,2000,228:22-36
|
CSCD被引
82
次
|
|
|
|
|
了解气象卫星更多信息，请访问