Allergen micro-array detection of specific IgE-reactivity in Chinese allergy patients
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文摘
Background Allergen micro-arrays are powerful tools for screening of serum IgE-reactivity. In this study allergen micro-arrays were used to identify dominating IgE-binding allergens and cross-reactivity patterns among selected Chinese allergy patients. Methods The study was conducted using patient sera from the cities of Guangzhou, Nanjing, Chengdu and Shenyang. In total 100 sera with Dermatophagoides pteronyssinus (Der p) specific IgE-levels higher than 50 kU/L were selected for testing against 103 individual allergens. Results Among 100 selected patients, 95% showed IgE-reactivity towards house-dust mite allergens Dermatophagoides farinae (Der f) 1, Der f 2 and Der p 2 and 94% were IgE positive against Der p 1, and 60% of sera contained IgE reacting against allergen Euroglyphus maynei (Eur m) 2. IgE against cat allergen, Felisdomesticus (Fel d) 1, was seen in 20%. Only 2% showed specific IgE-reactivity to Der p 10, a panallergen belonging to the tropomyosin family. Serum IgE-reactivity towards other allergens was in general low. IgE-reactivity against pollen allergens showed geographic differences. Conclusions This study clearly confirms that group 1 and group 2 are major allergens of house dust mites. These selected house-dust mite allergy patients are close to being mono-sensitized. Der p 10 is not an important allergen for cross-reactivity. Specific IgE-sensitization towards pollen allergens is low in southern China compared to other regions. The prevalence of food and stinging insect allergens known to give rise to IgE-mediated cross-reactivity is 2% or less.
来源
Chinese Medical Journal
,2011,124(24):4350-4354 【核心库】
DOI
10.3760/cma.j.issn.0366-6999.2011.24.039
关键词
specific immunoglobulin E
;
micro-array
;
Dermatophagoides pteronyssinus
;
Dermatophagoides farinae
;
cross-reactivity
地址
1.
Research Asia Pacific, ALK A/S, Guangdong, Guangzhou, 510230
2.
the First Affiliated Hospital, Guangzhou Medical College, State Key Laboratory of Respiratory Disease, Guangdong, Guangzhou, 510230
3.
Department of Respiratory, the Affiliated Children's Hospital of Nanjing Medical University, Jiangsu, Nanjing, 210008
4.
Department of Pediatrics, the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Sichuan, Chengdu, 610072
5.
Department of Allergy, the General Hospital of Shenyang Military Command, Liaoning, Shenyang, 110017
6.
Center for Clinical and Experimental Allergology, IDI-IRCCS, Italy, Italy, Rome
语种
英文
文献类型
简报
ISSN
0366-6999
学科
基础医学
文献收藏号
CSCD:4401040
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