青藏高原藏木香总黄酮提取工艺研究及含量测定
Study on the extraction technique and quantification of total flavonoids in Inula racemosa grown in Qinghai-Tibet plateau

杨月琴 ¹ 彭敏 ¹ 胡凤祖 ¹ 常小平 ² 马海乐 ³ 马世震 ¹ ^*

文摘	目的:研究藏木香中总黄酮的提取工艺及含量测定。方法:采用正交实验进行优选，考察乙醇浓度、溶剂倍数、提取时间等因素对黄酮得率的影响，用紫外分光光度法测定其含量。结果:乙醇浓度是主要的影响因素，最佳提取工艺为选用75%乙醇作为提取溶剂，料液比1:20，水浴热回流提取2h。以芦丁为对照品，采用分光光度法测定出总黄酮平均含量为0.28 mg/g。结论:提取工艺可行；紫外分光光度法简单、快速、准确；为藏木香深度开发提供了科学依据。
其他语种文摘	Objective:Aim to study the best conditions of extraction techniques for total flavonoids in Inula racemosa.Methods:The best conditions of extraction technique for total flavonoids were optimized with orthogonal experiment.Developed by ethanol solution with different concentrations,solvent multiples and extraction duration,determination of total flavonoids with UV- spectrophotometry was made.Results:Ethanol concentration was the major factor for determining total flavonoids contents.The best conditions for the extraction techniques of total flavonoids were:75% ethanol which was 20 times of the medicine amount,reflux extracting 2 hours.The yield of total flavonoids in Inula racemosa was estimated at 0.28mg/g.Conclusion:The extraction techniques are feasible and the UV-spectrophotometric method is simple,quick and accurate.
来源	食品工业科技 ,2011,32(2):251-253 【核心库】
关键词	藏木香 ; 黄酮 ; 提取工艺 ; 紫外分光光度法
地址	1. 中国科学院西北高原生物研究所, 青海, 西宁, 810008 2. 青海省大通宝库林场, 青海, 大通, 810100 3. 河南科技大学, 河南, 洛阳, 471003
语种	中文
文献类型	研究性论文
ISSN	1002-0306
学科	轻工业、手工业、生活服务业
基金	河南科技大学特聘教授科研启动费 ; 青海省重点攻关项目
文献收藏号	CSCD:4262438