藏药蕨麻多糖的光谱性质及单糖组成分析
Spectral Properties and Monosaccharides Compositon Analysis of Polysaccharide from Potentilla anserina L.

夏莲孙志伟李国梁索有瑞尤进茂 ^*

文摘	本研究对藏药蕨麻多糖进行了分离提纯,并测定其水溶性多糖含量为99.4%;通过紫外光谱与红外光谱分析表明,蕨麻多糖为分子量较小的α-吡喃糖,并含有氨基糖;蕨麻多糖的水解单糖经过NMP衍生后进行毛细管电泳分析,测得其单糖组成为木糖、阿拉伯糖、葡萄糖、鼠李糖、甘露糖、岩藻糖、半乳糖、葡萄糖醛酸和半乳糖醛酸,含量分别为3.945、77.445、17.568、17.646、3.942、2.165、65.268、13.037μg/mg和33.484μg/mg,与GC-MS的定性分析结果一致。
其他语种文摘	The polysaccharide of Potentilla anserine L.was extracted and purified,and its contents was determined as 98.4% by phenyl-sulfuric acid method.The polysaccharide was identified by IR spectrum and UV scanning spectrum.The IR spectrum indicates that the characteristic absorption peaks at 3600-3200,3200-2800,1400-1200,1200-1000 cm-1,and 845 cm-1 belonged the characteristic peak of α-pyranose.In addition,the peak at 1630 cm-1 assigned to the C=O of acetamide moiety stretching vibration meant that amino sugars exited in the polysaccharide.A method was developed for the separation of derivatized carbohydrates of Potentilla anserine L.using 1-naphthyl-3-methyl-5-pyrazolone(NMP)as derivatization reagent by capillary zone electrophoresis,and the results shows that the monosaccharides compositions of the polysaccharide from Potentilla anserine L.are xylose,arabinose,glucose,rhamnose,mannose,fucose,galactose,glucuronic acid and galacturonic acid with contents of 3.945,77.445,17.568,17.646,3.942,2.165,65.268,13.037 μg/mg,and 33.484 μg/mg,respectively,which are consistent with the results carried out by GC-MS.
来源	天然产物研究与开发 ,2011,23(3):453-457 【核心库】
关键词	蕨麻 ; 多糖 ; 光谱性质 ; 单糖组成
地址	中国科学院西北高原生物研究所, 西宁, 810001
语种	中文
文献类型	研究性论文
ISSN	1001-6880
学科	中国医学
基金	国家自然科学基金 ; 中国科学院“百人计划”项目
文献收藏号	CSCD:4250190