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中国对虾养殖群体与野生群体线粒体控制区序列的比较
Comparative analysis of the mitochondrial control region between the cultured and wild populations of Chinese shrimp (Fenneropenaeus chinensis)

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张辉 1   高天翔 1 *   庄志猛 2   金显仕 2  
文摘 比较分析了中国对虾养殖群体与野生群体mtDNA控制区序列.研究结果显示,在长度为563bp的mtDNA控制区片段中,中国对虾养殖群体与野生群体序列间存在一定程度的遗传差异.野生群体的基因多样度为0.9672,略高于养殖群体(0.9380),两群体间未检测到共享单倍型;野生群体mtDNA控制区核苷酸多样度为0.0106,养殖群体核苷酸多样度为0.0094,略低于野生群体的核苷酸多样度.基于K-2P模型计算得到中国对虾两群体间的平均遗传距离为0.0108,野生群体个体间平均遗传距离为0.0107,养殖群体个体间平均遗传距离为0.0095;单倍型最小跨度树和NJ系统树均未检测到显著的谱系结构.确切P检验显示两群体间没有随机交配现象(P=0.0009).两群体间的FST值为0.0698(P=0.00),表明两群体间存在显著的遗传分化
其他语种文摘 In present study, genetic differentiation of the Chinese shrimp Fenneropenaeus chinensis between cultured( 21 individuals) and wild populations( 14 individuals, sequences were downloaded from the GenBank) was investigated using the sequence of mitochondrial control region ( CR ). Four individuals of the F.merguiensis were chosen as the outgroup during the phylogenetic analysis in this study. Sequences were edited and aligned using DNA Star software and refined manually. Genetic diversity indices such as number of haplotypes, polymorphic sites, transitions, transversions, and indels were obtained using the program ARLEQUIN( Ver.3.0). Haplotype diversity( h) and nucleotide diversity( л) were calculated with DNASP 4.0.The nucleotide compositions and the average distances between populations and within population were calculated with MEGA 4.0.The Network software was used to draw the minimum spanning tree based on the haplotype data. The results were as follows:the A + T content was higher than the G + C content in both the populations, but the G + C content was a little lower in the cultured population. The results indicated genetic divergence existed between the 2 populations based on the 563 bp sequences. The gene diversity of the wild population( 0.967 2) was a little higher than the cultured population( 0.938 0). There was no shared haplotype between the 2 populations. The ratio of transitions/transversions of the wild populations and nucleotide diversity were 3.5 and 0.010 6 respectively while they were 4.0 and 0.009 4 for the cultured population. The nucleotide diversity of the wild population was a little higher than cultured population. The K-2P distance was 0.010 8 between the 2 populations,0.010 7 in the wild population and 0.009 5 in the cultured population. Both the NJ phylogenetic tree and the haplotype minimum spanning tree showed no significant genealogical structure. The exact test of population differentiation showed that the 2 populations were significantly different ( P=0.000 9) .The FST value between the populations was 0.069 8( P=0.00), which indicated that the difference between 2 populations was significant. This may indicate that some alleles were lost during the aquaculture
来源 水产学报 ,2010,34(8):1149-1155 【核心库】
DOI 10.3724/sp.j.1231.2010.06804
关键词 中国对虾 ; 线粒体控制区 ; 遗传多样性 ; 养殖群体 ; 野生群体
地址

1. 中国海洋大学, 教育部海水养殖重点实验室, 山东, 青岛, 266003  

2. 中国水产科学研究院黄海水产研究所, 山东, 青岛, 266071

语种 中文
文献类型 研究性论文
ISSN 1000-0615
学科 普通生物学;水产、渔业
基金 国家863计划 ;  国家海洋局公益性科研专项
文献收藏号 CSCD:4021793

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引证文献 11 篇

1 马朋 脊尾白虾3个野生群体线粒体COI基因的遗传多样性及其系统发育分析 渔业科学进展,2011,32(6):50-56
CSCD被引 9 次

2 牛东红 缢蛏种群遗传多样性的周年变异分析 生物技术通报,2012(1):168-171
CSCD被引 1 次

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