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Immunological rejection of acellular heterogeneous nerve transplant for bridging the sciatic nerve in rats

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文摘 BACKGROUND: Artificial materials composed of acellular heterogeneous nerves can resolve donor shortage problems for the repair of peripheral nerve defects. However, it remains unclear whether artificial materials can overcome immunological rejection of heterogeneous nerve grafts and obtain similar effects as allogeneic nerve grafts. OBJECTIVE: To analyze regeneration and immunological rejection of defective sciatic nerves in rats through the use of acellular heterogeneous nerve grafts. DESIGN, TIME AND SETTING: A randomized, controlled study was performed at the Department of Anatomy, China Medical University and the Experimental Center, First Affiliated Hospital, China Medical University between January and December 2008. MATERIALS: TritonX-100 (Sigma, USA) and deoxycholate (Pierce, USA) were used. METHODS: Bilateral sciatic nerves were collected from adult rabbits and treated with TritonX-100 and sodium deoxycholate to prepare acellular sciatic nerves, which were used to bridge 1-cm defective sciatic nerves in adult rats. MAIN OUTCOME MEASURES: The lymphocyte percentage in leukocytes was quantified following hemocyte staining. Neural regeneration and the recovery of motor end plates in the gastrocnemius muscle were observed under optical and electronic microscopy following toluidine blue staining, as well as acetylcholinesterase and succinate dehydrogenase histochemical staining. RESULTS: There was no significant difference in the lymphocyte percentage in leucocytes between transplanted and normal rats (P < 0.05). At 3 months after surgery, the rat toes on the operated side were separated and the rats could walk. In addition, the footplates exhibited an escape response when acupunctured. A large number of regenerated nerve fibers were observed in the transplant group, and acetylcholinesterase-positive motor end plates were visible in fibers of the gastrocnemius muscle. CONCLUSION: Acellular heterogeneous nerve transplants for the repair of defective sciatic nerves in rats promote neural regeneration without significant immunological rejection.
来源 Neural Regeneration Research ,2010,5(9):673-677 【核心库】
DOI 10.3969/j.issn.1673-5374.2010.09.006
关键词 heterogeneous nerve transplant ; peripheral nerve defect ; immunological rejection ; sciatic nerve ; neural regeneration
地址

1. Department of Neurosurgery, First Affiliated Hospital, China Medical University, Liaoning, Shenyang, 110001  

2. Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Liaoning, Shenyang, 110032  

3. Department of Human Anatomy, Basic College of Medical Sciences, China Medical University, Liaoning, Shenyang, 110001

语种 英文
文献类型 研究性论文
ISSN 1673-5374
学科 基础医学
基金 the Science and Technology Research Foundation of Liaoning Provincial Department of Education
文献收藏号 CSCD:3893811

参考文献 共 26 共2页

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引证文献 3 篇

1 Ma Xinlong Biomechanical properties of acellular sciatic nerves treated with a modified chemical method Neural Regeneration Research,2011,6(4):252-256
CSCD被引 5 次

2 Ma Xinlong Biomechanical properties of peripheral nerve after acellular treatment Chinese Medical Journal,2011,124(23):3925-3929
CSCD被引 0 次

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