Transplantation of olfactory ensheathing cells promotes axonal regeneration in a rat model of spinal cord injury
查看参考文献26篇
|
文摘
|
BACKGROUND: Transplantation of olfactory ensheathing cells (OECs) into the injured spinal cord has been shown to promote axonal regeneration and functional recovery. However, the mechanisms underlying the effects of OEC transplantation remain controversial. OBJECTIVE: To observe fibrotic scar formation and axonal regeneration in the damaged spinal cord following OEC transplantation, and to determine whether OEC transplantation promotes neural regeneration by attenuating fibrotic scar formation. DESIGN, TIME AND SETTING: A randomized, controlled animal experiment was performed at the Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Japan and at the Department of Human Anatomy, College of Basic Medical Sciences, China Medical University, China between April 2007 and May 2009. MATERIALS: OECs were obtained from olfactory nerves and olfactory bulbs of male, 4-week-old, Sprague Dawley rats. Rabbit anti-serotonin polyclonal antibody, rabbit anti-calcitonin gene-related peptide polyclonal antibody, rabbit anti-glial fibrillary acidic protein polyclonal antibody, rabbit anti-type IV collagen polyclonal antibody, and mouse anti-rat endothelial cell antigen-1 monoclonal antibody were used. METHODS: Male, Sprague Dawley rats aged 8 weeks were randomly divided into three groups: sham-surgery (n = 3), surgery (n = 9), and OEC transplantation (n = 11). Spinal cord transection at the T_(9-10) level was performed and the rats were transplanted with a 2-μL (1 × 10~5 cells) cell suspension. MAIN OUTCOME MEASURES: Formation of glial and fibrotic scars was examined using immunohistochemistry for glial fibrillary acidic protein and type IV collagen. Serotonin-positive and calcitonin gene-related peptide-positive axons were visualized by immunohistochemistry, respectively. Double immunofluorescence for type IV collagen and rat endothelial cell antigen-1 was also performed to determine co-localization of type IV collagen deposition and blood vessels. RESULTS: At 1 week after spinal cord injury, numerous glial cells were observed around the lesion site. Formation of fibrotic scar was determined by a large amount of type IV collagen deposition in the lesion center, and descending serotonin- or ascending calcitonin gene-related peptide- containing axons stopped at the fibrotic scar that was formed in the lesion site. At 1 week after transplantation, the formation of fibrotic scar was significantly inhibited. In addition, the fibrotic structure was partly formed and centralized in the blood vessel, and serotonergic and calcitonin gene-related peptide-containing axons were regenerated across the lesion site. CONCLUSION: OEC transplantation into the injured spinal cord attenuated fibrotic scar formation and promoted axon regeneration. |
|
来源
|
Neural Regeneration Research
,2010,5(9):651-656 【核心库】
|
|
DOI
|
10.3969/j.issn.1673-5374.2010.09.002
|
|
关键词
|
fibrotic scar
;
olfactory ensheathing cell transplantation
;
spinal cord injury
;
neural regeneration
;
rats
|
|
地址
|
Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, Japan, Fuchu, 183-8526
|
|
语种
|
英文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1673-5374 |
|
学科
|
基础医学 |
|
基金
|
国家自然科学基金
;
Scientific Research Foundation of Technology Department of Liaoning Province in China
|
|
文献收藏号
|
CSCD:3893807
|
参考文献 共
26
共2页
|
|
1.
Willerth SM. Cell therapy for spinal cord regeneration.
Adv Drug Deliv Rev,2008,60(2):263-276
|
CSCD被引
16
次
|
|
|
|
|
2.
Bunge MB. Bridging the transected or contused adult rat spinal cord with Schwann cell and olfactory ensheathing glia transplants.
Prog Brain Res,2002,137:275-282
|
CSCD被引
3
次
|
|
|
|
|
3.
Barnett SC. Olfactory ensheathing cells (OECs) and the treatment of CNS injury: advantages and possible caveats.
J Anat,2004,204(1):57-67
|
CSCD被引
17
次
|
|
|
|
|
4.
Raisman G. Repair of neural pathways by olfactory ensheathing cells.
Nat Rev Neurosci,2007,8(4):312-319
|
CSCD被引
18
次
|
|
|
|
|
5.
Ramer LM. Peripheral olfactory ensheathing cells reduce scar and cavity formation and promote regeneration after spinal cord injury.
J Comp Neurol,2004,473(1):1-15
|
CSCD被引
33
次
|
|
|
|
|
6.
Stichel CC. Inhibition of collagen IV deposition promotes regeneration of injured CNS axons.
Eur J Neurosci,1999,11(2):632-646
|
CSCD被引
3
次
|
|
|
|
|
7.
Klapka N. Suppression of fibrous scarring in spinal cord injury of rat promotes long-distance regeneration of corticospinal tract axons, rescue of primary motoneurons in somatosensory cortex and significant functional recovery.
Eur J Neurosci,2005,22(12):3047-3058
|
CSCD被引
10
次
|
|
|
|
|
8.
Kawano H. Inhibition of collagen synthesis overrides the age-related failure of regeneration of nigrostriatal dopaminergic axons.
J Neurosci Res,2005,80(2):191-202
|
CSCD被引
6
次
|
|
|
|
|
9.
Homma A. Differential response of arcuate proopiomelanocortin- and neuropeptide Y-containing neurons to the lesion produced by gold thioglucose administration.
J Comp Neurol,2006,499(1):120-131
|
CSCD被引
1
次
|
|
|
|
|
10.
Li HP. Regeneration of nigrostriatal dopaminergic axons by degradation of chondroitin sulfate is accompanied by elimination of the fibrotic scar and glia limitans in the lesion site.
J Neurosci Res,2007,85(3):536-547
|
CSCD被引
1
次
|
|
|
|
|
11.
Teng X. Regeneration of nigrostriatal dopaminergic axons after transplantation of olfactory ensheathing cells and fibroblasts prevents fibrotic scar formation at the lesion site.
J Neurosci Res,2008,86(14):3140-3150
|
CSCD被引
3
次
|
|
|
|
|
12.
Ramón-Cueto A. Long-distance axonal regeneration in the transected adult rat spinal cord is promoted by olfactory ensheathing glia transplants.
J Neurosci,1998,18(10):3803-3815
|
CSCD被引
11
次
|
|
|
|
|
13.
Ramón-Cueto A. Functional recovery of paraplegic rats and motor axon regeneration in their spinal cords by olfactory ensheathing glia.
Neuron,2000,25(2):425-435
|
CSCD被引
11
次
|
|
|
|
|
14.
Lu J. Transplantation of nasal olfactory tissue promotes partial recovery in paraplegic adult rats.
Brain Res,2001,889(1/2):344-357
|
CSCD被引
45
次
|
|
|
|
|
15.
Lu J. Olfactory ensheathing cells promote locomotor recovery after delayed transplantation into transected spinal cord.
Brain,2002,125(Pt 1):14-21
|
CSCD被引
36
次
|
|
|
|
|
16.
Ramón-Cueto A. Olfactory ensheathing glia: properties and function.
Brain Res Bull,1998,46(3):175-187
|
CSCD被引
4
次
|
|
|
|
|
17.
Lipson AC. Neurotrophic properties of olfactory ensheathing glia.
Exp Neurol,2003,180(2):167-171
|
CSCD被引
35
次
|
|
|
|
|
18.
Liu JB. The mitosis and immunocytochemistry of olfactory ensheathing cells from nasal olfactory mucosa.
Chin J Traumatol,2005,8(5):306-310
|
CSCD被引
6
次
|
|
|
|
|
19.
García-Alías G. Acute transplantation of olfactory ensheathing cells or Schwann cells promotes recovery after spinal cord injury in the rat.
J Neurosci Res,2004,75(5):632-641
|
CSCD被引
2
次
|
|
|
|
|
20.
Camand E. Long-term changes in the molecular composition of the glial scar and progressive increase of serotoninergic fibre sprouting after hemisection of the mouse spinal cord.
Eur J Neurosci,2004,20(5):1161-1176
|
CSCD被引
2
次
|
|
|
|
|
了解气象卫星更多信息，请访问