小鸡脑片突触反应长时程增强的细胞内记录和多参数分析
Intracellular recordings and multi-parameter analysis of long-term potentiation of synaptic responses in chick brain slices
查看参考文献18篇
|
文摘
|
本文旨在建立日龄小鸡脑片制备及其神经元细胞内记录技术,观察中间腹内侧原皮质(intermediate medial mesopallium,IMM)神经元的电学特性、突触反应及其长时程增强(long-term potentiation,LTP)现象.取2～10日龄小鸡制备左侧前脑切片(500 μm厚),对69个IMM神经元进行细胞内记录,测得静息电位(-59.4±5.3)mV、膜斜率电阻(70.8±27.2)MΩ、时间常数(10.2±4.3)ms,动作电位幅度(85.2±9.4)mV、阈电位(-38.7±7.6)mV、超射(25.6±8.9)mV、半幅时程(2.1±0.5)ms、最大上升斜率(150.5±41.2)mV/ms、最大下降斜率(-64.3±14.0)mV/ms.其中32个神经元的放电频率随刺激电流强度增大而升高[直线同归斜率为(21.5±10.9)Hz/nA,均P<0.05].在所记录IMM神经元的背侧(n=25)、腹侧(n=62)局部电刺激(0.1 Hz)均可诱发具有强度、膜电位依赖性的去极化突触反应(即EPSP).对12个测试细胞给予腹侧局部强直刺激(5 Hz,300脉冲/串,2串,串间隔10 min),在6个细胞诱导出EPSP的LTP,呈现为EPSP的幅度、曲线下面积、时程和最大上升斜率均增大(强直刺激后45 min与强直刺激前相比,P<0.05,n=5),但相应的膜电学特性无明显变化(P＞0.05).以上结果表明,在日龄小鸡脑片上建立的IMM神经元细胞内记录技术稳定可靠,可用于突触反应LTP的多参数分析. |
|
其他语种文摘
|
Day-old chick is unique animal model in brain development and behavior study. The intermediate medial mesopallium (IMM), a region of the chick forebrain, is intimately involved in the early learning processes, which offers the ideal opportunity to study the neural changes that underlie behavioral plasticity. In this paper, the intracellular recordings were conducted from IMM neurons in chick forebrain slices, in which electrophysiological properties, synaptic responses and long-term potentiation (LTP) were observed. Coronal sections of left forebrains (500 μm thick), containing IMM, were prepared from domestic chicks, aged 2-10 days. In 69 IMM neurons, the resting membrane potential was measured to be (-59.4±5.3)mV, slope membrane resistance (70.8±27.2) MΩ, and time constant (10.2±4.3) ms. The amplitude, threshold, overshoot, half-width, max rise slope and max decay slope of action potential evoked by intracellular current injection were (85.2±9.4) mV, (-38.7±7.6) mV, (25.6±8.9) mV, (2.1±0.5) ms, (150.5±41.2) mV/ms and (-64.3±14.0) mV/ms, respectively. Spike-firing frequency was increased with depolarizing current intensity in 32 of 69 tested cells [linear regression slope was (21.5±10.9) Hz/nA, P<0.05 in all cells]. The depolarizing synaptic responses (i.e. EPSPs), with stimulus intensity- and membrane potential-dependent properties, were elicited by dorsal (n=25) or ventral (n=62) focal electrical stimuli at 0.1 Hz in all tested IMM neurons and could be nullified reversibly by perfusion with 100μmol/L AP5(NMDA receptor antagonist)and 3 μmol/L DNQX(non-NMDA receptor antagonist),but enlarged by 6 μmol/L bicuculline(GABA_A receptor antagonist).The EPSPs evoked by ventral stimulation were persistently increased after tetanic stimulation(5 Hz,300 pulses/train,2 trains,train interval 10 min)in 6 of 12 tested IMM neurons.The amplitude of EPSPs was potentiated to more than 120%of control level(when analyzed at 45 min of enhancement,P<0.05,n=5),which lasted at least 30 min and then could be refefred to as LTP Moreover area under curve,duration and max rise slope of EPSPs were also enhancexl(P<0.05),while no significant changes werf observcd in the electrophysiological parameters of IMM neurons following induction of LTP(P>0.05).These results suggest that the intracellular recording techniques in the chick brain slices can be used to perform multi-parameter analysis of synaptic responses and their LTP. |
|
来源
|
生理学报
,2009,61(6):577-584 【核心库】
|
|
关键词
|
小鸡
;
离体
;
端脑
;
突触传递
;
长时程增强
;
电生理学
;
膜电位
|
|
地址
|
1.
中国科学院心理研究所, 中国科学院心理健康重点实验室, 北京, 100101
2.
皖南医学院细胞电生理研究室, 安徽, 芜湖, 241002
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0371-0874 |
|
学科
|
基础医学 |
|
基金
|
国家自然科学基金
;
安徽省自然科学基金
|
|
文献收藏号
|
CSCD:3789226
|
参考文献 共
18
共1页
|
|
1.
管林初. 利用小鸡建立学习和模型的方法介绍.
心理学报(Chinese,English abstract),1991,23(3):319-324
|
CSCD被引
9
次
|
|
|
|
|
2.
McCabe BJ. Effects of restricted lesions of the chick forebrain on the acquisition of filial preference during imprinting.
Brain Research,1981,205(1):29-37
|
CSCD被引
1
次
|
|
|
|
|
3.
Davies DC. The effects of hyperstriatal lesions on 1-trial passive avoidance learning in the chick.
Journal of Neuroscience,1988,8(12):4662-4666
|
CSCD被引
1
次
|
|
|
|
|
4.
Payne JK. Long-term consequences of exposure to an imprinting stimulus on 'spontaneous' impulse activity in the chick brain.
Behavioural Brain Research,1984,13(2):155-162
|
CSCD被引
1
次
|
|
|
|
|
5.
Bradford CM. Neuronal activity related to memory in the intermediate and medial part of the hyperstriatum ventrale (IMHV) of the chick.
Brain Research,1994,640(1/2):11-16
|
CSCD被引
1
次
|
|
|
|
|
6.
Stewart MG. Morphological changes associated with stages of memory formation in the chick following passive avoidance training.
Behavioural Brain Research,1995,66(1/2):21-28
|
CSCD被引
4
次
|
|
|
|
|
7.
Bradley PM. The effects of age and visual experience on potentiation of responses in slices from the chick forebrain.
PROCEEDINGS.BIOLOGICAL SCIENCES/THE ROYAL SOCIETY,1991,243(1306):25-30
|
CSCD被引
1
次
|
|
|
|
|
8.
Bradley PM. Electrophysi-ological correlates of past history:in vitro studies of the IMHV of the domestic chick.
Behavioural Brain Research,1999,98(2):261-265
|
CSCD被引
3
次
|
|
|
|
|
9.
Bradley PM. Local circuitry in the IMHV of the domestic chick (Gallus domesticus).
Proceedings of the Royal Society of London Series B:Biological Sciences,1990,240(1299):479-492
|
CSCD被引
1
次
|
|
|
|
|
10.
汪萌芽. 离体运动神经元对腹外侧索刺激的突触反应特征.
生理学报(Chinese,English abstract),1997,49(6):625-631
|
CSCD被引
4
次
|
|
|
|
|
11.
蒋娟. Concentration-dependent actions of ketamine on CA1 pyramidal neurons of rat hippocampus in vitro.
皖南医学院学报(Chinese,English abstract),2002,21(4):244-246
|
CSCD被引
1
次
|
|
|
|
|
12.
Bradley PM. Morphological and electrophysiological properties of neurons in an area of the chick brain involved learning.
Brain Research,1996,727(1/2):125-132
|
CSCD被引
1
次
|
|
|
|
|
13.
Bradley PM. Potentiation of synaptic responses in slices from the chick forebrain.
PROCEEDINGS.BIOLOGICAL SCIENCES/THE ROYAL SOCIETY,1991,243(1306):19-24
|
CSCD被引
1
次
|
|
|
|
|
14.
Liu XJ. Analysis of high-frequency stimulation-evoked synaptic plasticity in mouse hippocampal CA1 region.
生理学报,2008,60(2):284-291
|
CSCD被引
1
次
|
|
|
|
|
15.
Whitlock JR. Learning induces long-term potentiation in the hippocampus.
SCIENCE,2006,313(5790):1093-1097
|
CSCD被引
42
次
|
|
|
|
|
16.
Matsushima T. D,-receptor dependent synaptic potentiation in the basal ganglia of quail chicks.
NEUROREPORT,2001,12(13):2831-2837
|
CSCD被引
2
次
|
|
|
|
|
17.
Wang X. Synaptic potentiation and depression in slices of mediorostral neostriatum-hyperstriatum complex,an auditory imprinting-relevant area in chick forebrain.
NEUROSCIENCE,1994,60(3):689-699
|
CSCD被引
2
次
|
|
|
|
|
18.
Margrie TW. Long-term potentiation of synaptic transmission in the avian hippocampus.
Journal of Neuroscience,1998,18(4):1207-1216
|
CSCD被引
4
次
|
|
|
|
|
了解气象卫星更多信息，请访问