高压氧治疗后血管性痴呆大鼠梨状皮层神经发生和血液供应变化
Neurogenesis and blood supply in piriform cortex in vascular dementia rats after hyperbaric oxygen therapy
查看参考文献8篇
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文摘
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目的 探讨血管性痴呆(vascular dementia,VD)大鼠高压氧治疗后梨状皮层(piriform cortex,Pir)神经发生及血液供应情况.方法 SD雄性成年大鼠分为正常组、血管性痴呆组(VD组)、血管性痴呆高压氧治疗组(HBO组;高压氧治疗10 d),每组10只,CT灌注成像技术检测各组大鼠Pir区局部脑血流量(regional cerebral blood flow,rCBF)、局部脑血容量(regional cerebral blood volume,rCBV)和平均通过时间(mean transit time,MTT),穿梭箱系统检测大鼠学习记忆功能,免疫组化技术研究Pjr区Nestin及doubleeortin(DCX)表达情况.结果 VD组较正常组大鼠Pir区CBF下降,CBV增加,MTT延长;HBO组较VD组Pir区CBF上升,局部CBV下降,MTT缩短;HBO组Pir区CBF、CBV、MTT各值与正常组接近;Pir区Nestin阳性细胞主要位于layer Ⅱ和layer Ⅲ,阳性表达强度:HBO组＞VD组＞正常组;Pir区DCX阳性细胞成条带状,主要位于嗅裂(rf)和杏仁裂(af)之间layer Ⅱ,阳性表达强度:正常组＞HBO组＞VD组;HBO组穿梭箱AAR比率(49.5±9.3)%较VD组(27.5±7.9)%显著增高(P＜0.01);VD组大鼠AAR比率(27.5±7.9)%显著低于正常组(87.5±7.9)%(P＜0.01).结论 高压氧治疗能够改善VD大鼠认知功能障碍并促进Pir区神经发生,此町能和高压氧治疗提高VD大鼠Pir区血液供应有关. |
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其他语种文摘
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Objective To explore the neurogenesis and blood supply in the piriform cortex of rat with vascular dementia after hyperbaric oxygen therapy. Methods SD male rats (at the age of 2 months) were inflicted by permanent ligation of bilateral carotid arteries (2VO). In 30 d after the surgery, the animals having a percentage less than 60% in active avoidance response (AAR) in shuttle box testing were identified as suffering from vascular dementia (VD). Twenty VD rats were divided randomly into 2 groups; VD group and hyperbaric oxygen group [ HBO group, in a tank of 95% oxygen under a pressure of 2. 02 × 10~5 Pa (2ATA), 120 min/d, for 10 d]. Another 10 normal rats served as control. In 10 d after the treatment, all rats undertook shuttle box testing for their ability of learning and memory. Their regional cerebral blood flow (rCBF), regional cerebral blood volume (rCBV) and mean transit time (MTT) in the piriform cortex were measured by CT perfusion imaging. The expressions of Nestin and doublecortin (DCX) in the piriform cortex were detected by immunohistochemical staining. Results There was significant difference in AAR rate of shuttle box testing between HBO group and VD group [ (49.5 ±9. 3)% vs (27. 5 ±7. 9)%, P＜0.01 ]; Significant difference was also seen in AAR rate between VD group and NC group [ (87.5 ±7.9)%, P＜0.01 ]. In VD group, the rCBF was declined, rCBV elevated, and MTT was delayed in comparison with the normal control. While hyperbaric oxygen therapy improved rCBF, decreased rCBV, and shortened MTT compared with VD group. The values of rCBF, rCBV and MTT in HBO group were close to those in normal control. In the piriform cortex,Nestin~+ cells mostly located in the layer II and layer Ⅲ, and the intensity of positive cells was HBO grouP＞ VD grouP＞ normal control; DCX~+ cells were arrayed as a belt mostly in the layer U between amygdaline fissure (af) to rhinal fissure (rf). Conclusion HBO therapy enhances the ability of learning and memory and promotes neurogenesis in the piriform cortex of VD adult rats. |
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来源
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第三军医大学学报
,2009,31(9):809-812 【核心库】
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关键词
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血管性痴呆
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梨状皮层
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神经发生
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高压氧
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CT灌注
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地址
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第三军医大学大坪医院野战外科研究所神经内科, 重庆, 400042
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第三军医大学大坪医院野战外科研究所放射科, 重庆, 400042
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第三军医大学大坪医院野战外科研究所高压氧科, 重庆, 400042
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中国科学院心理健康重点实验室, 中国科学院心理健康重点实验室, 北京, 100101
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-5404 |
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学科
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医药、卫生;临床医学;神经病学与精神病学 |
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基金
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重庆市自然科学基金
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文献收藏号
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CSCD:3580562
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