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文摘
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采用HPLC法同时测定了藏药达乌里秦艽中龙胆苦苷(gentiopicroside)、獐牙菜苦苷(swertiamarin)、獐牙菜苷(sweroside)和落干酸(loganic acid)4种主要环烯醚萜苷类成分,利用在线质谱(MS)进行了定性分析,并与同属藏药麻花艽进行了比较。色谱柱为Eclipse XDB-C_8(4.6 mm×150 mmi.d.,5μm),流动相A:体积分数5%乙腈(含10 mmol甲酸)水溶液,流动相B:体积分数95%乙腈水溶液,以流动相A在0～15 min内由100%线性减至90%,15～20 min内保持90%不变线性梯度洗脱。流速1.0 mL/min,检测波长240 nm,柱温30℃。结果表明,4种成分均达到了基线分离,具有较好的线性关系和回收率。本法可作为达乌里秦艽药材有效成分的测定方法,为其有效成分的进一步开发利用提供依据。 |
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其他语种文摘
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A simple and reliable method for the simultaneous quantitative determination of four iridoid glycosids gentiopicroside,swertiamarin,sweroside and loganic acid in a famous Tibetan medicine Gentiana dahurica Fischer by HPLC was developed.Identification of four analytes was performed by on line post-column mass spectrum(APCI).Comparison with another Tibetan medicine G.straminea in the same sampling time was also discussed.All the analysis were performed on Eclipse XDB-C_8(4.6 mm×150mm i.d.,5 μm) at column temperature of 30 ℃ with a good baseline resolution and eluted with the solution of mobile phase A: 5% acetonitrile(including 0.01 mol methanoic acid) and mobile phase B: 95% acetonitrile.The proportion of A and B changed from 100∶0 to 90∶10 in 0～15 min and 90∶10 in 15～20 min.The flow rate was 1.0 mL/min.Good linear regression equations(r≥0.9990) within test ranges and recoveries were obtained.The analytical method was simple,accurate,and could be used for the quantitative determination and quality control of this plant medicine G.dahurica. |
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来源
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分析试验室
,2008,27(12):51-54 【核心库】
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关键词
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藏药
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达乌里秦艽
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环烯醚萜苷
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HPLC
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地址
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中国科学院西北高原生物研究所, 青海省青藏高原特色生物资源研究重点实验室, 青海, 西宁, 810001
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语种
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中文 |
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文献类型
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简报 |
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ISSN
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1000-0720 |
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学科
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化学 |
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基金
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国家中西部专项(2001BA901A47)项目资助
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文献收藏号
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CSCD:3434553
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