2-(11H-苯[a]咔唑)乙基氯甲酸酯柱前衍生化用于氨基酸的高效液相色谱分析
Application of 2- ( 11H-benzo [ a ] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column rivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection
查看参考文献14篇
文摘
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利用新型荧光试剂2-(11H-苯[a]咔唑)乙基氯甲酸酯(BCEC-Cl)作为柱前衍生化试剂,在HypersilBDS C_(18)(200mm×4.6mm,5μm)反相色谱柱上,采用梯度洗脱对20种氨基酸衍生物进行了分离检测。在乙腈与Na_2B_4O_7缓冲液中,室温下BCEC-Cl与氨基酸反应5min可实现完全衍生。检测的激发和发射波长分别为λ_(ex)=279nm,λ_(em)=380nm。采用柱后质谱电喷雾离子源(ESI source)正离子模式,实现了水解牛血清白蛋白中氨基酸和油菜蜂花粉中氨基酸的定性定量检测。荧光定性检测的线性回归系数均大于0.9990,检出限为1.49~19.74fmol(S/N=3)。 |
其他语种文摘
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On a reversed phase Hypersil BDS C_(18) (200 mm × 4.6 mm, 5 μm) column, 20 amino acids, which were derivatized using 2-( 11H-benzo [ a ] carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) as precolumn derivatization reagent, were separated in conjunction with a gradient elution. Optimum derivatization was obtained by reacting of amino acids with BCEC-Cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. The fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. The identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. Linear correlation coefficients of the amino acid derivatives were 〉 0. 9990, and detection limits ( at signal to noise of 3:1 ) were 1.49 - 19.74 fmol for the labeled amino acids. |
来源
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分析化学
,2008,36(8):1071-1076 【核心库】
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关键词
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高效液相色谱-质谱
;
荧光检测
;
柱前衍生
;
氨基酸
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蜂花粉
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地址
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1.
中国科学院西北高原生物研究所, 青海, 西宁, 810001
2.
曲阜师范大学化学科学学院, 曲阜, 273165
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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0253-3820 |
学科
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化学;生物化学 |
基金
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国家自然科学基金
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文献收藏号
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CSCD:3376440
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