外源NO与蔗糖对盐胁迫下番茄（Lycopersicon esculentum Mill）幼苗氧化损伤的保护效应
Protective effects of NO and sucrose on oxidative damage in tomato （ Lycopersicon esculentum Mill） seedling leaves under NaCI stress

苏桐 ¹ 魏小红 ² 丁学智 ³ 李源 ²

文摘	选取长至6～8片真叶的健康番茄（Lycopersicon esculentum Mill）幼苗,分别进行蔗糖、硝普钠（sodium nitropresside,SNP,作为外源NO供体）及其体积比例组合（1∶1）处理;36h后施以NaCl胁迫,并分别于0h（胁迫前）、24h、48h和72h取样,进行相关生理生化指标测定。具体5个实验处理如下：A.蒸馏水（CK）;B.100 mmol/L NaCl;C.0.1 mmol/L SNP＋100 mmol/L NaCl;D.0.1 mmol/L SNP＋1.0mmol/L蔗糖＋100 mmol/L NaCl;E.1.0 mmol/L蔗糖＋100 mmol/L NaCl。结果表明：与SNP和蔗糖单独处理相比,二者组合处理对缓解盐胁迫下番茄幼苗的氧化损伤存在正协同效应,主要表现在进一步增强了番茄幼苗超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）的活性;提高了脯氨酸（Pro）的含量,同时膜脂过氧化产物丙二醛（MDA）含量显著降低（P〈0.05）。采用聚丙烯酰胺浓度梯度凝胶电泳对盐胁迫24 h和48 h材料的POD同功酶检测表明,当NaCl单独处理时,番茄幼苗叶片POD同功酶第V条带缺失,其它谱带酶量减少,抑制了POD同功酶的表达;SNP和蔗糖单独处理能够保护盐胁迫（24、48h）所导致的POD同功酶条带的完整;而组合处理既保证了POD同功酶条带的完整,又加强了酶量的表达。随着盐胁迫时间的延长,其氧化损伤程度愈烈,SNP和蔗糖组合处理能够更有效地缓解盐胁迫对番茄幼苗植株造成的氧化损伤。
其他语种文摘	Tomato seedling with 6 -- 8 leaves was used to investigate impact of nitric oxide （NO） and sucrose on oxidative damage to young tomato leaves under NaCl stress. Sodium nitropresside （SNP） was used as NO donor. The tomato seedlings were grouped and then sprayed on surface of leaves with solutions of distilled water, NaCl, SNP, sucrose and SNP ＋ sucrose （ 1 ： 1 ）. After spraying 36h, the leaves were subjected to NaCl stress at 100 mmol/L for 24h, 48h and 72h respectively. The detail treatments as follows： A. CK（distilled water） ;B. 100 mmol/L NaCl;C. 0.1 mmol/L SNP ＋ 100mmol/L NaCl;D. 0. 1 mmol/L SNP ＋ 1.0mmol/Lsucrose ＋ 100 mmol/L NaCl; E. 1.0 mmol/Lsucrose ＋ 100 mmol/L NaCl. The results indicated that there were protective function to tomato seedling leaves from NaCl oxidative damage when applications of SNP, sucrose and their mix solutions. However, the treatment-D showed a much better protective impact than the treatments of C and E as it could significantly increase activities of antioxidant enzymes （including SOD, CAT, POD, APX and GR） and content of proline, while largely decrease MDA content in tomato seedling leaves under salt stress. Moreover, the effect of salt stress on the peroxidase enzyme expression in tomato leaves was further analyzed by using polyacrylamide concentration gradient gel electrophoresis technique. The results showed that salt stress could inhibit the expression of small molecule POD isoenzymes. The mix solution of SNP and sucrose could largely promote POD isoenzyme activity and protective them from hydrolyzation than single application of either SNP or sucrose solutions. Irrespective of solution, the POD isoenzymes expression was higher at 24h than 48h salt stress. The mix solution of SNP and sucrose could have a big potential for reduction of oxidative damage to tomato seedling leaves under salt stress
来源	生态学报 ,2008,28(4):1558-1564 【核心库】
关键词	一氧化氮 ; 蔗糖 ; 番茄（Lycopersicon ; esculentum ; Mill）幼苗 ; NaCl胁迫 ; 氧化损伤
地址	1. 甘肃农业大学农学院, 甘肃, 兰州, 730070 2. 甘肃农业大学生命科学技术学院, 甘肃, 兰州, 730070 3. 中国科学院西北高原生物研究所, 青海, 西宁, 810008
语种	中文
文献类型	研究性论文
ISSN	1000-0933
学科	植物学
基金	甘肃省教育厅基金资助项目（0702-14;032B-01） ; 国家教育部高等学校优秀青年教师教学科研奖励计划
文献收藏号	CSCD:3272327