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文摘
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枯草芽孢杆菌JA因产生多种脂肽类化合物而具有广阔的开发前景.JA发酵液经过离心、酸沉淀、甲醇抽提等步骤得到脂肽类化合物的粗提物.将粗提物溶于流动相,采用反相高效液相色谱分离,对收集的洗脱峰组分进行电喷雾质谱(ESI-MS)分析.根据质荷比推断JA菌株产生的脂肽类化合物属于3个家族,分别为surfactin, iturin和fengycin,是枯草芽孢杆菌合成的重要生物表面活性素.对一级质谱中的主成分进行串联质谱分析,进一步确定了3种脂肽类化合物的分子结构.实验证明ESI-MS是一种鉴定脂肽类化合物及其同系物的可靠方法. |
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其他语种文摘
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Bacillus subtilis JA produces a broad spectrum of bioactive lipopeptides with great potential applications in agriculture and industry. Crude lipopeptides were extracted with 100% methanol from the precipitate which was obtained by adding 6 mol/L HCl to the cell-free culture broth. The crude extract was separated on a Diamonsil C18 column (5 μm, 250 mm×4.6 mm) in reversed-phase high performance liquid chromatography (HPLC) system to separate the lipopeptide homologues. Five peaks were eluted from HPLC. Electrospray ionization mass spectrometry (ESI-MS) was used to analyze each HPLC fraction. The results showed three series of ion peaks. According to the values of m/z, the three series of ions were classified into surfactin, iturin and fengycin homologues, which were well-known biosurfactants produced by B. subtilis strains. The major ions were structurally characterized using tandem mass spectrometry. This study suggested a reasonable method for the isolation and identification of lipopeptides produced by B. subtilis strains. |
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来源
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色谱
,2008,26(3):343-347 【核心库】
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关键词
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高效液相色谱-电喷雾质谱法
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枯草芽孢杆菌
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脂肽类化合物
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地址
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中国科学院离子束生物工程学重点实验室, 中国科学院离子束生物工程学重点实验室, 安徽, 合肥, 230031
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-8713 |
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学科
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化学 |
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基金
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国家自然科学基金
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文献收藏号
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CSCD:3267886
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