|
|
RP-HPLC测定藏木香中土木香内酯和异土木香内酯含量
Determination of Alantolactone and Isoalantolactone in Herb Inula racemosa Hook.f.by RP-HPLC
查看参考文献8篇
|
文摘
|
目的 建立了反相高效液相色谱法同时测定藏木香中土木香内酯和异土木香内酯的方法.方法 采用Phenomenex Kromasil C18色谱柱(4.6mm×250mm,5.0μm);流动相为乙腈-0.04%磷酸溶液(50:50);检测波长:194 nm;流速:1.0 mL·min~(-1).结果 异构体土木香内酯和异土木香内酯达到基线分离,含量测定的线性良好,线性范围和相关系数分别为0.07～4.80μg·L~(-1) (r=0.999 8),0.07～4.85μg·L~(-1) (r=0.999 8);回收率分别为97.5%和102.1%;方法精密度良好,RSD分别为1.56%和1.87%(n=5);方法重现性良好,RSD分别为1.67%和0.92%(n=5).结论 所建立的方法简便、快捷、准确,重现性好,可用于藏木香药材及其制剂的质量控制. |
|
其他语种文摘
|
To establish a quantitative RP-HPLC method for the simultaneous determination of alantolactone and isoalantolaclone in herb Inula racemosa Hook. f. METHODS A column of Phenomenex Kromasil C_(18) (4.6 mm × 250 mm, 5.0 μm)was used. The mobile phase consisted of acelonitrile and 0.04% phosphate (50:50). The flow rate was 1.0 mL·min~(-1) with UV detection wavelength at 194 nra. RESULTS Isomer alantolactone and isoalantolaclone showed good separable degree. A good linearity was obtained with the correlation coefficients ranged of 0.07～4.80μg·L~(-1)(r=0.999 8) and 0. 07～4.85 μg·L~(-1) (r =0. 999 8). The average recoveries were 97.5% and 102. 1%. The precision and accuracy of the assay were 1.56%and 1.87% (n =5). CONCLUSION The method is rapid, accurate, reliable and can be used to control the quality of herb Inula racemosa Hook. f. and it products. |
|
来源
|
中国药学杂志
,2007,42(7):491-493 【核心库】
|
|
关键词
|
反相高效液相色谱法
;
藏木香
;
土木香内酯
;
异土木香内酯
;
含量测定
|
|
地址
|
中国科学院西北高原生物研究所, 西宁, 810001
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1001-2494 |
|
学科
|
中国医学 |
|
文献收藏号
|
CSCD:2725458
|
参考文献 共
8
共1页
|
|
1.
.
中国药典2005年版.一部,2005:13
|
CSCD被引
1
次
|
|
|
|
|
2.
LUO D S.
中华藏本草,1997:254-255
|
CSCD被引
1
次
|
|
|
|
|
3.
WANG K T. Separation and determination of alantolactone and isoalantolactone in traditional Chinese herbs by capillary electrophoresis.
Talanta,2000(52):1001-1005
|
CSCD被引
1
次
|
|
|
|
|
4.
WANG X L. GC determination alantolactone in herb lnula helenium L.
山东中医药大学学报,2005,29(4):322-323
|
CSCD被引
1
次
|
|
|
|
|
5.
XIA J. Determination of isomers in Radix Inulae by GC.
中国药学杂志,2005,40(24):1895-1896
|
CSCD被引
1
次
|
|
|
|
|
6.
LI G L. Assay alantolactone in herb Inula helenium L.by thin layer scanning.
中国实验方剂学杂志,2002,8(6):62-63
|
CSCD被引
1
次
|
|
|
|
|
7.
YAN Z J. Assay isoalantolactone in herb Inula helenium L.by thin layer scanning.
中国民族医药杂志,1999,12(5):132
|
CSCD被引
1
次
|
|
|
|
|
8.
YUAN Z B. On the elect rochemical behavior of alantolactone.
分析测试学报,1998,17(2):47-49
|
CSCD被引
1
次
|
|
|
|
|
|
了解气象卫星更多信息，请访问