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文摘
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目的 比较正常大鼠和多柔比星心肌病大鼠心肌组织部分基因表达谱的差异,探讨甘肃黄芪对上述基因表达谱的影响.方法 30只Wistar大鼠随机分3组:对照组即生理盐水组(n=10),多柔比星组(n=10)和黄芪+多柔比星组(n=10).分别从3组心肌组织中抽提总RNA,用Cy3,Cy5荧光标记,经逆转录合成动物来源的eDNA探针;eDNA探针与4000点基因表达谱芯片杂交,结果由软件分析表达信号.结果 共有923条基因出现差异表达,其中586条基因表达下调,337条基因表达上调.凋亡相关基因表达在多柔比星心肌病时上调;氧化和能量代谢相关基因的表达在多柔比星心肌病时下调.6个在多柔比星组上调的基因在黄芪+多柔比星组下调,8个在多柔比星组下调的基因在黄芪+多柔比星组上调,3个在多柔比星组上调的基因在黄芪+多柔比星组表达进一步增强.结论 凋亡和能量代谢障碍及免疫因素在多柔比星心肌病的发病机制中参与作用,甘肃黄芪对多柔比星诱导的心肌病大鼠的心脏保护作用是通过多基因多途径调节相关基因表达而实现的. |
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其他语种文摘
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OBJECTIVE To investigate the difference of genetic expression spectra between doxorubicin-indueed cardiomyopathy in rats and normal controls by DNA microarray. To explore the effects of Radix Astragali Gansuensis on gene expression profiles in rals with doxorubicin-indueed cardiomyopalhy. METHODS Thirty Wislar rats were divided randomly into 3 groups, i.e control group (n=10) , duxorubicin group(n=10) and Radix Astragali + doxorubicin group(n=10). cDNA probes were prepared by labeling the mRNA extracted from myocardium and marked with Cy3-dTP and Cy5-dUTP respectively through reverse transaction. DNA microarray were construcled by spoiling PCR products of 4 000 and oDNAs onto specially treated glass slides and were then hybridized against, the cDNA probes followed by fluorescent signals scanning. RESULTS Nine hundred and twenty three genes were detected with differential expressions. The expressions of 586 genes were found to be down-regulated and 337 ones up-regulated. Energy metabolism-related genetic expressions were down-regulated in tissues of rats with doxorubicin-indueed cardiomyopathy compared with normal tissue. Expressions of apoptosis-related genes were up-regulaled in tissues of doxorubicin-indueed cardiomyopalhy. Six up-regulated and 8 down-regulated genes in doxorubicin group were down-regulated and up-regulated in Radix Astragali + doxorubicin group respectively. Expressions of 3 up-regulaled genes in doxorubicin group were further enhanced in Radix Astragali + doxorubicin group. CONCLUSION Apoptosis, energy metabolism disorder and immunity may participate the pathogenesis changes in rals with doxorubicin-induced cardiomyopathy. Radix Astragali Gansuensis can protect the heart in rats with doxonibicin-induced cardiomyopathy. Multiple gene expressions are involved to implement this pharmacological effect. |
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来源
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中国药学杂志
,2007,42(6):437-441 【核心库】
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关键词
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多柔比星
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黄芪
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心肌病
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基因表达谱
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地址
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1.
兰州大学第二医院心内科, 兰州, 730030
2.
中国科学院西北高原生物研究所测试中心, 西宁, 810001
3.
中国科学院兰州化物所, 甘肃省天然药物重点实验室, 兰州, 730000
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1001-2494 |
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学科
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药学 |
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基金
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甘肃省自然科学基金
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甘肃省科学事业费项目
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文献收藏号
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CSCD:2712561
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