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RP-HPLC Determination and Pharmacokinetic Comparison of Cinnamic Acid in Rat Plasma After Administration of Di-Gu-Pi Decoction and Pure Cinnamic Acid
查看参考文献6篇
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文摘
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A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase （HPLC） system with a Diamonsil C18 column and methanol-acetonitfile-water （8： 32： 60, volume ratio） （adjusted to pH = 3.0 with glacial acetic acid） as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-（p-fluoro-phenyl）-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0. 10 to 25.0μg/mL （R2 = 0. 9988, n = 9）. The precision was 3.42%-10. 10%; the between-day precision was 2. 84%-8.91% ; the accuracy was - 1.51%-1.26% ; the mean recovery was 99. 9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid. |
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来源
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Chemical Research in Chinese Universities
,2006,22(1):56-60 【核心库】
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DOI
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10.1016/s1005-9040(06)60045-7
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关键词
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Di-Gu-Pi decoction
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Cinnamic Acid
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Pharmacokinetics
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RP-HPLC
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地址
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1.
Department of Pharmacy, Shenzhen Hospital，Beijing University, Shenzhen, 518036
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College of Pharmacy，Shenyang Pharmaceutical University, Shenyang, 110016
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1005-9040 |
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学科
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药学 |
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文献收藏号
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CSCD:2659490
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