慢性冷水游泳应激对大鼠海马、前脑皮层神经颗粒素含量的影响
The changes of neurogranin level in rat hippocampus and forebrain after chronic cold water swimming stress
查看参考文献14篇
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文摘
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目的探讨慢性应激对大鼠海马和前脑皮层神经颗粒素（NG）含量的影响。方法将雄性SD大鼠随机分为应激组（予10℃冷水游泳应激，共2周）、装置对照组和正常对照组，每组10只。于应激前后测量三组大鼠的体质量变化情况，并记录每天应激过程中应激组和装置对照组的排便量以考察应激的强度，应激后以Western blotting方法测定海马和前脑皮层中的NG含量（以NG含量与β-Actin含量的比值表示）。结果（1）应激前后，三组大鼠的体质量比较，差异均无统计学意义（P〉0．05），但应激组的体质量增长相对缓慢；（2）应激第4-14天应激组的排便量多于装置对照组（P〈0．01）；（3）应激组和装置对照组海马的NG含量[分别为（0．66±0．13）和（0．94±0．26）]低于正常对照组[（1．93±0．53）]，差异有统计学意义（P〈0．01）；（4）应激组前脑皮层的NG含量[（0．45±0．00）]低于装置对照组和正常对照组[分别为（2．76±1．74）和（2．87±1．63）]，差异有统计学意义（P〈0．01）；（5）应激组海马NG含量高于前脑皮层（P〈0．01）；装置对照组海马NG含量低于前脑皮层（P〈0．01）；正常对照组海马与前脑皮层NG含量的差异无统计学意义。结论慢性应激导致海马和前脑皮层NG含量显著下降；对于时程长、程度严重、适应不良的冷水游泳应激，前脑皮层受损比海马更为严重；而对于时程长、程度微弱的装置应激，海马则比前脑皮层更为敏感。 |
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其他语种文摘
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Objective To investigate the effects of chronic cold water swimming stress on protein levels of neurogranin in rat hippocampus and forebrain, and to understand the central mechanisms of stress from the molecular level. Methods Thirty SD rats were randomly divided into handled-controls, apparatus-controls and stressed groups, with ten in each group. Forced lOt cold water swimming was used as a stressor. The defecation numbers of both stressed rats and apparatus controls were recorded during 5-minute trial on each day. Furthermore, the weight of rats was monitored during the periods of stress as an index reflecting degree of stress. The neurogranin level in the hippocampus and forebrain after stress was measured with the Western blotting. Results (1) Although there was no significant difference in final body weight among the three groups, less weight gain was in stressed rats than in controls. (2) In comparison with the apparatus controls, the stressed group had significantly more numbers of defecation during a 5-minute trial on the 4th to 14th experiment day (P <0. 01) , and no significant differences were found between two groups on the experiment day 1 to 3 (P >0. 05). (3) the hippocampal protein levels of neurogranin in stressed group (0. 66 ±0. 13) and apparatus-controls (0. 94 ±0. 26) were significantly lower than that in handled-controls (1. 93 ±0. 53) (P < 0. 01). (4) The forebrain protein levels of neurogranin in stressed group (0.45 ± 0. 00) were significantly lower than that in apparatus-controls (2. 76 ± 1. 74) and handled-controls (2. 87 ± 1.63) (P<0. 01). (5) The protein levels of neurogranin in hippocampus were significantly higher than that in forebrain (P <0. 01) in stressed group. However, the protein levels of neurogranin in apparatus- controls was significantly higher in forebrain than in hippocampus (P < 0. 01). There was no significant difference between forebrain and hippocampal neurogranin levels in handled-controls. Conclusion Chronic swimming stress can significantly decrease the protein levels of neurogranin in hippocampus and forebrain, with more severe changes in the forebrain than in the hippocampus. However, hippocampus seems to be more sensitive than forebrain in response to chronic mild apparatus stress. |
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来源
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中华精神科杂志
,2005,38(3):174-177 【核心库】
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关键词
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应激
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海马
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前脑
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大脑皮质
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神经粒素
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地址
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中国科学院心理研究所脑-行为研究中心, 北京, 100101
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1006-7884 |
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学科
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神经病学与精神病学 |
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基金
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国家自然科学基金资助项目
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中国科学院知识创新工程项目
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文献收藏号
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CSCD:1995224
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参考文献 共
14
共1页
|
|
1.
Lupien S J.
Behav Brain Res,2001,127:137-158
|
CSCD被引
6
次
|
|
|
|
|
2.
张艳美. 慢性应激对大鼠海马CA3区锥体细胞形态结构的效应.
中华精神科杂志,2002,35:22-24
|
CSCD被引
26
次
|
|
|
|
|
3.
马强.
中国应用生理学杂志,2001,16:318-320
|
CSCD被引
1
次
|
|
|
|
|
4.
McEwen B S.
Clin Neurosci Res,2001:19-34
|
CSCD被引
1
次
|
|
|
|
|
5.
Watson J B.
Proc Natl Acad Sci U S A,1992,89:8581-8585
|
CSCD被引
6
次
|
|
|
|
|
6.
Wu J.
J Biol Chem,2002,277:19498-19505
|
CSCD被引
17
次
|
|
|
|
|
7.
Houben M P.
J Neurosci Res,2000,59:750-759
|
CSCD被引
7
次
|
|
|
|
|
8.
Neuner-Jehle M.
Brain Res,1995,685:143-153
|
CSCD被引
8
次
|
|
|
|
|
9.
McEwen B S.
Brain Res,2000,886:172-189
|
CSCD被引
22
次
|
|
|
|
|
10.
Palanza P.
Neurosci Biobehav Rev,2001,25:219-233
|
CSCD被引
10
次
|
|
|
|
|
11.
Chen C C.
Brain Res,1994,648:65-72
|
CSCD被引
5
次
|
|
|
|
|
12.
韩太真.
学习与记忆的神经生物学,1998:208-209
|
CSCD被引
4
次
|
|
|
|
|
13.
McEwen B S.
Biol Psychiatry,2000,48:721-731
|
CSCD被引
20
次
|
|
|
|
|
14.
Conrad C D.
Behav Neurosci,1999,113:902-913
|
CSCD被引
15
次
|
|
|
|
|
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