增强UV-B辐射对麻花艽叶片的抗氧化酶的影响
Effects of Enhanced UV-B Radiation on Antioxydative Enzymes in Gentiana straminea Leaves
查看参考文献20篇
|
文摘
|
以青藏高原的特有植物麻花艽为材料,研究麻花艽叶片抗氧化酶系统对增强UV-B辐射的响应.结果表明:在UV-B处理初期,麻花艽叶片SOD、POD的酶活性都能增加,但随着处理时间的延长,SOD、POD的活性呈现下降趋势.麻花艽叶片CAT的酶活性在UV-B处理后下降明显,但作为清除叶绿体中H2O2的关键酶AP的酶活性表现为明显地增加趋势,说明在对麻花艽叶片增强UV-B辐射反应中AP起有着重要作用.MDA的含量随UV-B处理时间延长而增加,表明UV-B降低了细胞内活性氧自由基的清除能力,膜脂过氧化作用加剧,导致了对麻花艽叶片的伤害效应. |
|
其他语种文摘
|
Effects of enhanced UV-B radiation on the activities of SOD, POD, CAT and AP in Gentiana straminea Maxim, leaves,were studied. The results showed that in Gentiana straminea Maxim, leave the activities of superoxide dismutase (SOD) and peroxidase (POD) increased under the enhanced UV-B radiation,and decreased in long terms UV-B exposure ;at the same time, in Gentiana struminea Maxim, leave the activities of catalase (CAT) droped and the activity of avscorbic acid-peroxidase (AP),as a key enzyme of HZO2 scavenging system in choloroplasts ,enhanced under the enhanced UV-B radiation. It might be showed that AP was mostly useful in the effects of antioxydative enzyme by enhanced UV-B radiation in Gentiana straminea Maxim, leaves. The content of malonaldehyde (MDA) was markedly enhanced by long terms UV-B exposure in Gentiana straminea Maxim, leaves. It might be concluded that UV-B injury to membrane was correlated to activated oxygen accumulation which promoted lipid peroxidation, while excessive activated oxygen production might be caused by the inhibition of antioxydative enzyme. |
|
来源
|
西北植物学报
,2005,25(3):519-524 【核心库】
|
|
关键词
|
麻花艽
;
UV-B
;
抗氧化酶
|
|
地址
|
中国科学院西北高原生物研究所, 青海, 西宁, 810001
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1000-4025 |
|
学科
|
植物学 |
|
基金
|
国家自然科学基金
;
国家科技部国际合作重点项目
;
中国科学院知识创新工程项目
|
|
文献收藏号
|
CSCD:1961500
|
参考文献 共
20
共1页
|
|
1.
郭松. 青海高原大气O↓3及紫外辅射 UV-B 观测结果的初步分析.
科学通报,1994,39:50-53
|
CSCD被引
15
次
|
|
|
|
|
2.
MADRONICH S. Changes in ultraviolet radiation reaching the earths surface[J].
Ambio,1995,24(1):143-152
|
CSCD被引
25
次
|
|
|
|
|
3.
GIANNOPOLITIS C N. Superoxide dismutase Ⅱ.
Plant Physiol,1997,59:315-318
|
CSCD被引
40
次
|
|
|
|
|
4.
ORMAN R G. Peroxide.
Plant Physiol,1980,65:407-408
|
CSCD被引
19
次
|
|
|
|
|
5.
CHANCE B. Assay of catalase and perxidase[J].
Methods Enzymol,1955,2:764-775
|
CSCD被引
308
次
|
|
|
|
|
6.
NAKANO Y. Peroxide is scavenged by ascorbate-specific in spinach chloroplasts[J].
Plant Cell Physiol,1981,22:867
|
CSCD被引
888
次
|
|
|
|
|
7.
BRADFORD M M. A rapid and sensitive method for the quantization of microgramquantities of protein utilizing the principle of protein-dye binding[J].
Anal Biochem,1976,72:248
|
CSCD被引
7667
次
|
|
|
|
|
8.
RAERI A. Gultathioe-ascorbic acid cyclein pumplein plans grown under polluted air in open-up chambers[J].
Plant Physiol,1993,142(3):286-290
|
CSCD被引
4
次
|
|
|
|
|
9.
FRIDOVCH I. a history[A].
Superoxide dismutase(Vol.Ⅰ),1982:89-124
|
CSCD被引
1
次
|
|
|
|
|
10.
王以柔. 低温诱导水稻幼苗的光氧化伤害.
植物生理学报,1990,16(2):102-108
|
CSCD被引
20
次
|
|
|
|
|
11.
王宝山. Biological free radicals and membrane damage of plants[J].
植物生理学通讯,1988,2:12-16
|
CSCD被引
239
次
|
|
|
|
|
12.
刘宛. Effects of NaCl stress and exogenous free radical on oxygen free radical and membrane lipid peroxidation of isolated wheat leaves[J].
植物生理学通讯,1995,31(1):26-29
|
CSCD被引
28
次
|
|
|
|
|
13.
李韶山. UV-B对水稻幼苗膜脂过氧化作用的影响.
激光生物学报,2000,9(1):23-26
|
CSCD被引
12
次
|
|
|
|
|
14.
晏斌. 紫外线B对水稻叶组织中活性氧代谢及膜系统的影响.
植物生理学报,1996,22:373-378
|
CSCD被引
64
次
|
|
|
|
|
15.
郭培国. 夜间高温胁迫对水稻叶片光合机构的影响.
植物学报,2000,42(7):673-678
|
CSCD被引
73
次
|
|
|
|
|
16.
GRODEN D. H2O2 destruction by ascorbate-dependent systems from chloroplasts[J].
Biochim. Biophys. Acta,1979,546:426
|
CSCD被引
5
次
|
|
|
|
|
17.
曹宛红. Ascorbate peroxidase as a key enzyme of H2O2 scavenging system in choloroplasts[J].
植物生理学通讯,1994,30(6):452-458
|
CSCD被引
2
次
|
|
|
|
|
18.
刘志礼. NaCl胁迫对螺旋藻生长及抗氧化酶活性的影响.
植物学通报,1998,15(3):43-47
|
CSCD被引
20
次
|
|
|
|
|
19.
MURPHY T F. Membrame as targets of ultraviolet radiation[J].
Plant Physiol,1983,58:381-388
|
CSCD被引
7
次
|
|
|
|
|
20.
曾韶西. 低温胁迫对黄瓜子叶抗环血酸过氧化物酶活性和谷胱甘肽含量的影响.
植物生理学报,1990,16(1):37-42
|
CSCD被引
45
次
|
|
|
|
|
了解气象卫星更多信息，请访问