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Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite(APSAC) and Its Thrombolytic Properties
查看参考文献12篇
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文摘
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The dialysis method has been traditionally used for the conversion of native human plasminogen (GluHpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for the preparation of an acyl-plasminogen-streptokinase activator complex(APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquidphase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8. 8 h in rabbits. |
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来源
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Chemical Research in Chinese Universities
,2004,20(5):584-587 【核心库】
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关键词
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Acyl-plasminogen-streptokinase activator complex (APSAC)
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Solid-phase synthesis
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Thrombolytic
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地址
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1.
College of Life Science, Jilin University, Changchun, 130023
2.
Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, 110016
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1005-9040 |
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学科
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化学 |
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基金
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吉林省自然科学基金
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吉林大学创新基金
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文献收藏号
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CSCD:1844816
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