藏药秦艽、麻花艽中落干酸和龙胆苦甙的HPLC法含量测定
Content Determination of Loganic Acid and Gentiopicroside in Tebitan Herbal Medicines Gentiana macrophylla and G. straminea by HPLC

林鹏程 ¹ 冶兆辉 ¹ 卢永昌 ¹ 赵素琴 ¹ 胡凤祖 ² 纪兰菊 ²

文摘	应用反相高效液相色谱法同时测定藏药秦艽、麻花艽中落干酸、龙胆苦甙含量.并比较了加热回流提取及超声提取两种方法对分析结果的影响.还测定了两种藏药全草及根、茎、叶、花等不同部位两种成分的含量.
其他语种文摘	A quantitative method to determine loganic acid and gentiopicroside in Gentiana macrophylla Pall, and Gentiana straminea Maxim, by RP-HPLC was established in this paper. The two compounds were base-isolated on the coloumn of Zorbax SB-C_(18) (250 mm * 4. 6 mm i. d., 5 μm) which eluted with MeOH and H_2O(0. 04% H_3PO_4). The methol ratio increased from 18% to 28% in 15 min with detective wave at 254 nm and eoloumn temperature at 3 ℃, flow rate at 1 ml/min. A wide range of linearity and the good linear relationships were achieved on above condition. The contents of the two compounds in different parts of the two plants were also determined and compared in this paper.
来源	中药材 ,2004,27(11):819-821 【扩展库】
关键词	反相高效液相色谱 ; 落干酸 ; 龙胆苦甙 ; 秦艽 ; 麻花艽
地址	1. 青海民族学院化学系, 青海, 西宁, 810007 2. 中国科学院西北高原生物研究所, 青海, 西宁, 810001
语种	中文
文献类型	研究性论文
ISSN	1001-4454
学科	中国医学
文献收藏号	CSCD:1677266