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低频超声对卵巢癌COC1细胞增殖和凋亡的影响
Influence of Low Frequency Ultrasound on Proliferation and Apoptosis of Human Ovarian COC1
查看参考文献10篇
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文摘
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目的观察低频超声对人卵巢癌COC1体外生长的影响.方法以低频超声(频率0.24 MHz,剂量5.12W/cm~2*5s)辐照COC1细胞,采用MTT观察超声对COC1细胞增殖的抑制作用;流式细胞仪(FCM)、TUNEL法和光、电镜检测细胞凋亡.结果超声波能够显著抑制卵巢癌COC1细胞增殖,辐照后12h、24h、36h和48h,其MTT光吸收值与对照组相比显著降低;FCM检测超声辐照后6h的COC1细胞凋亡率达(25.57±4.68)%;同时TUNEL法观察到棕褐色着染的凋亡细胞;相差显微镜及电镜下可见凋亡小体形成等典型形态学改变.结论低频超声辐照对人卵巢癌COC1细胞体外生长具有明显的抑制作用,并可诱导其凋亡. |
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其他语种文摘
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Objective To investigate the effect of Low-Frequency Ultrasound on proliferation and apoptosis of human ovarian cell line COCI. Methods The growth inhibitory effect of Ultrasound Exposure(0.24 MHz, 5.12 W/cm~2 * 5 s) on COCI cell was observed by MTT colormetric assay; Cell apoptosis was analyzed using flow cytometry (FCM); Terminal deoxynucleotidyl transferase-mediated dUTP-digoxin nick end labeling (TUNEL); Phase contrast light microscope and Electron microscope were applied to detect the morphological changes of COCI cell. Results Sonication could obviously inhibit the proliferation of COCI cell; The absorbance value(As7o)of MTT significantly decreased at 12h,24h,36h and 48h after sonication as compared to control group; The apoptosis rate was found to reach (25. 57 ± 4.68) % by FCM, and brown-colored positive apoptotic cells were observed with TUNEL method at 6h after sonication; Typical morphological changes of apoptosis such as apoptotic body were found under Phase contrast light microscope and Transmission electron microscope. Conclusion The results suggest Low-Frequency Ultrasound can suppress proliferation and induce apoptosis in vitro in human ovarian cell line COCI. |
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来源
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肿瘤防治研究
,2004,31(5):249-251 【扩展库】
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关键词
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超声
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细胞凋亡
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COC1细胞
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地址
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重庆医科大学医学超声工程研究所, 重庆市超声医学工程重点实验室, 400016
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-8578 |
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学科
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肿瘤学 |
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基金
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国家自然科学基金资助项目
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文献收藏号
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CSCD:1584535
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