A蛋白定向固定抗体用于椭偏光学生物传感器免疫检测
Oriented Immobilization of Human IgG by Protein A on Imaging Ellipsometry Biosensor
查看参考文献9篇
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文摘
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椭偏光学生物传感器是在椭偏光学显微成像技术的基础上发展的一项生物传感技术.它能够直接观测固体表面上的生物分子面密度,毋需任何标记辅助,适合发展成为一种无标记免疫检测技术.研究了在硅片表面上通过A蛋白定向固定抗体分子用于椭偏光学生物传感器免疫检测的可能性.实验结果表明,通过A蛋白固定抗体得到的抗体膜层的均一性和固定量的重复性能够保证椭偏光学生物传感器免疫检测结果的质量.通过A蛋白定向固定的抗体的抗原结合位点趋向一致,显著提高了抗体与抗原结合的能力.此外,通过蛋白A固定的免疫球蛋白G分子能够结合更多的多克隆抗体分子说明通过A蛋白固定的蛋白质分子能够较好地保持其空间构象. |
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其他语种文摘
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The biosensor based on optical imaging ellipsometry, can be used lo detect directly, without labeling, the surfaceconcentration of biomolecules on solid surface. The feasibility of using protein A to immobilize antibody on the silicon surface ofthe imaging ellipsometry biosensor was investigated in this study. The results showed that the anli-IgG immobilized by the proteinA on silicon surface could bind effectively human IgG, and the human IgG immobilized on silicon surface by protein A boundmore polyclonal antibody molecules than that immobilized on silicon surface directly, suggesting that prolein A might block thesurface to prevent the absorption of human IgG on surface directly, which might compromise its native configuration. The siliconsurface modified with protein A is expected to be used to immobilize a variety of antibodies, as prolein A can bind selectively theFe regions of many mammalian IgG. The combination of imaging ellipsometry and the protein A surface modification has the po-tential to be developed into immunoassays of high sensitivity. |
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来源
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生物工程学报
,2004,20(1):111-114 【核心库】
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关键词
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A蛋白
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椭偏光学生物传感器
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无标记免疫检测技术
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地址
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中国科学院力学研究所, 国家微重力实验室, 北京, 100080
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-3061 |
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学科
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生物物理学 |
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基金
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国家自然科学基金
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中国科学院项目
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文献收藏号
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CSCD:1508317
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